NA transformation of E. coli: The two plasmids were added to individual tubes containing E. coli and one with no plasmids. The three samples of E. coli were heated in a 42°C water bath for 90 seconds to heat shock the bacteria so that the plasmids would be taken up by the E. coli. These samples were then incubated at 30°C for half an hour and then plated on LB agar. Each tube was plated on an LB plate and a LB + ampicillin plate. Ampicillin is an antibiotic that is effective against E. coli‚ both
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sdsadsdsThe Price To Pay • When Filipinos shop‚ the amount of money they have to shell out is still a very important factor. Ninety-two percent plan their shopping in advance‚ and 79 percent prepare a shopping list before hitting the supermarkets or any place they want to do their shopping. They look for cheaper options and prioritize buying the basics: 73 percent allot time to look for the best promotions‚ and even if 89 percent decide what brand to buy before shopping‚ 81 percent end up choosing
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Migration When the Light of Home is Abroad: Unskilled Female Migration and the Filipino Family * The article focuses on the Filipino female population migrating to Singapore and the difficulties they face while in their host country. Through interviews and extensive research the authors explore the relationships between Filipino migrant women and their families. The authors state‚ “Specifically‚ we explore how migrant women and their family members define and negotiate family ideals
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Jose Rizal published Sobra la Indolencia de los Filipinos on July 15 to September 15‚ 1890 in five installments through the La Solidaridad‚ the official newspaper of the Philippine ilustrados(“enlightened ones”) – those who hoped to have a representation of the Filipinos in the Spanish Courts. This essay is also the longest among his essays. Through Rizal’s deep insights of social anthropology‚ he was able to analyze the factors causing the Filipinos’ indolence. According to him‚ that was not due
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waves of The Filipino Americans immigration to the US as well as their contribution. In what ways are the experiences of the Filipino different and similar to that of other Asian Americans? Show that you are familiar with the information from the text in your answer. Coming from a country of seven thousand plus islands and a culture where " women were considered equal to men‚"(1) according to Linda A. Revilla in her article entitled‚ "Filipino Americans: Historical Review‚" Filipino Americans have
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How can I best represent myself as a Filipino seafarer? WORLD CLASS indeed‚ that’s what a Filipino seafarer is. But what is a seafarer by the way. According to www.pinoymaritime.com‚ a seafarer is a traveler who goes by the sea. Or even a person who works near the ocean. Seafarers come from all over the world spending their lives close to the seas as they find life at sea to be a unique existence. They come from different backgrounds and cultures‚ and they work in varied areas of the shipping
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SUMMARY OF Indolence of the Filipinos Indolence of the Filipinos (“La Indolencia de los Filipinos”) The essay itself originally appeared in the Filipino forthrightly review‚ La Solidaridad‚ of Madrid‚ in five installments‚ running from July 15 to September 15‚ 1890. It was a continuation of Rizal’s campaign of education in which he sought by blunt truths to awaken his countrymen to their own faults at the same time that he was arousing the Spaniards to the defects in Spain’s colonial system
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alcohol. Alarmingly‚ in Southeast Asia‚ Filipinos were found to be the second highest consumers of alcohol‚ second only to the Indonesians. In the study‚ beer appears to be the favorite drink of Filipinos due‚ principally to its affordability compared to hard drinks. Based on the latest available data‚ however‚ Filipinos are seen as the No. 1 wine drinkers in the whole of the Asian region. The prevalent rate of alcohol consumption is estimated at 5 million Filipino drinkers in a population of 90 million
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I am Filipino “ Can they smell that aroma in me?” Every race around the world has different culture and beliefs and in that difference sometimes they even fight because of that contrast. One of the races known around the world is the Filipino. The Filipino people (Filipino: Mamamayang Pilipino) or Filipinos are an ethnic group native to the islands of the Philippines. According to the 2010 Census‚ there were 92‚337‚852 in the Philippines and about 10-12 million living outside the Philippines. There
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Assignment: Chapter 5 1. Besides the commercial and religious goals of Spain in colonizing the Philippines‚ give two other reasons (political and legal) why Spain claimed the country as its possession or the property of the King of Spain. * They also claim the country because of their Spirit of Discovery to our country‚ improvements of their technologies (for travel and trading purposes) and acquisition of our territories. 2. What is meant by “crown colony?” Can you give an example of a present
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