Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
Premium Ethanol Glucose Carbon dioxide
The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
Premium Laboratory glassware Laboratory equipment Chemistry
concentrations of solutes in and out of the cell. Filtration is what is used to remove solid particles and they can be removed by passing through a liquid and gas. The information above is very important because it is exactly what everything in this lab will be about. It explains in detail what every single little definition and important
Premium Osmosis Diffusion Membrane biology
BACK TITRATION- DETERMINATION OF THE CARBONATE CONTENT IN GARDEN LIME NAME: OSEI BONSU ERIC ID: 3906409 EXPERIMENT: I.2.2.1.
Premium Calcium carbonate Titration Calcium
Column Chromatography of Plant Pigments Paul Ibarbia‚ Gene Paolo Jasmin‚ Gianpaolo Jimenez and Lorenzo Labicane* Department of Biology‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Column chromatography of plant pigments is the separation of plant pigments extracted from Capsicum frutescens (siling labuyo). No slurry is prepared of the eluent; eluent with the stationary phase is set and then carefully poured into the column. The eluent are the solvents which are responsible for the
Premium Chromatography Gas chromatography
mixture 2.4971 g Salicylic Acid Crystallization Mass of weighing paper + Salicylic acid 1.7468 g Mass of weighing paper 0.9446 g Mass of Salicylic Acid 0.8022 g Calculation of percent salicylic Acid 32.13 % in original mixture: % salicylic acid = (m Salicylic Acid : m unknown mixture) x 100% = (0.8022 : 2.4971) x 100% = 32.13% CuSO4.5H2O Crystallization Mass of weighing paper + CuSO4.5H2O 2.6484 g Mass of weighing paper 1.0127 g Mass of CuSO4.5H2O 1.6357 g Calculation of percent
Premium Solubility Solvent Ethanol
total of 15 points. To receive full credit for Exercise 8‚ you must submit the following: 1) The typed Exercise 8 report form‚ complete with all of your data recorded and questions answered. 2) A full scientific report for the Part I experiment that your group conducted. Use the supplemental guide to writing a scientific report to help you produce this report. Part 1: Does light matter? Table 8.1: Group and Class rates for Photosynthesis by Elodea sprigs. |Light Condition
Premium Photosynthesis Plant Plant physiology
ABSTRACT Separation and identification of components of a mixture was determined by using Chromatography. The components of a mixture have different affinities for a stationary phase as well as different affinities for a mobile phase. INTRODUCTION An extremely common technique by using two chemicals and filter paper can give you sufficient information. A well-done Chromatography of dyes will provide you with enough data to determine if a liquid mixture is heterogeneous or homogenous . In a learning
Premium Dye Food coloring Analytical chemistry
Investigation Investigate the amount of heat evolved when magnesium reacts with dilute acids. Planning What I am going to do I am going to find out how much heat is given out when magnesium reacts with a variety of dilute acids. In order to make comparisons between the acids‚ I shall use my results to work out how much heat would be given out if 1 mole of magnesium reacted with an excess of each acid. The acids I shall use are hydrochloric acid‚ sulphuric acid‚ nitric acid and ethanoic acid
Premium Hydrogen Sulfuric acid Acid
The Charge/Mass (e/m) Ratio of the Electron PHYS 0212: Introduction to Laboratory Physics Fall 2012 Abstract The experiment conducted demonstrated correlation between the charge and mass of an electron and the behavior of magnetic fields. The lab was divided into four parts. The first three parts were conducted with a compass that was used to locate the magnetic field lines around a bar magnet‚ a solenoid and then a pair of Helmholtz coils. As a result of these trials‚ our observations found
Premium Magnetic field