Paper chromatography is a useful technique for separating and identifying pigments and other molecules from cell extracts that contain a complex mixture of molecules. The solvent moves up the paper by capillary action‚ which occurs as a result of the attraction of solvent molecules to the paper and the attraction of solvent molecules to one another. As the solvent moves up the paper‚ it carries along any substances dissolved in it. The pigments are carried along at different rates because they
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Chromatography of Commercial Analgesics Using the thin layer chromatography the major components of a commercial analgesic tablet will be indentified and purified. The active ingredients of the analgesic used were characterized by comparison with chromatograms of aspirin‚ acetaminophen‚ ibuprofen‚ mefanemic acid‚ and caffeine through the use of UV chromatography then the Rf value was calculated for each solvent. INTRODUCTION In this experiment‚ thin-layer chromatography (TLC) will be used
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Objectives: To use the process of chromatography to separate plant pigments. To compare the plant pigments found in spinach and red leaf lettuce leaves. Hypotheses: I believe the spinach leaf in the acetone will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. I believe the spinach leaf in distilled water will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. Discussion Questions:
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exchange Chromatography Ion exchange chromatography is a process for separating proteins and other molecules in a solution based on differences in net charge. Ion Exchange Chromatography relies on charge-charge interactions between the proteins in your sample and the charges immobilized on the resin of your choice. Ion exchange chromatography can be subdivided into cation exchange chromatography‚ in which positively charged ions bind to a negatively charged resin; and anion exchange chromatography‚ in
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Determining the concentration of red dye #40 in an unknown solution Introduction: White light is composed of many different wavelengths of light combined together. A spectrophotometer is an instrument that shines a single wavelength of light of a known intensity into a solution and then measures the intensity of the light exiting the solution. If a solution contains
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ARTICLE TRADITIONAL DYE PLANTS OF MANIPUR Sanjeev Rana Knitwear Department‚ National Institute of Fashion Technology Abstract Manipur is one of the richest states in plant biodiversity in the North Eastern states of India. Different ethnic groups‚ residing in Manipur before the introduction of the chemical dyes into the state‚ used the dyes extracted from the plants. Women in Manipur practice dyeing using varieties of plant leaves‚ flowers and tree barks. The natural dyes were obtained from
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It was concluded that the product created was paracetamol when a thin layer chromatography test was performed and observed under ultraviolet light (which is discussed further down the page). An unknown substance was treated with acetic anhydrate and resulted with paracetamol. A compound that behaves in this matter is 4-aminophenol and is widespread in the industrial production of this drug. Upon these observations was the build up of “Chemical A” decided. When 4-aminophenol is t treated with
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Biology 103 Lab Spring 2013 Does a larger head mean higher intelligence? Introduction: Some say a larger head gives room for a bigger‚ more intelligent brain. Men‚ thought to have larger heads simply because they’re… well men. In which case‚ men have a larger head size than women‚ therefore making men smarter than women. Methods: My partner and I went ahead and measured the heads of 15 people in our class‚ 5 of them male and 10 of them female. We used a measuring band to measure‚ to
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Purpose: The purpose of this lab is to find the amount of blue dye #1 in the blueberry pomegranate gatorade. Procedure: We created dilutions using stock solution(10mL stock/0 mL water‚ 8mL/2mL‚ 6mL/4mL‚4mL/6mL‚ 3mL/7mL‚ 2mL/8mL‚1mL/9mL‚ 0mL/0mL). Before we put each dilution in the SPEC 20‚ we put water in a cuvette and put it in the SPEC 20 so we could zero out the percent transmittance. We collected the percent transmittance of each dilution using the SPEC20 with a wavelength of 630nm.
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Chromatography Experiment of the Drosophila Eye Pigmentation Pathway Introduction: How can the use of chromatography help explain single gene mutations of the drosophila melanogaster bio-synthetic pigment pathway? This experiment was meant to help connect the mode of inheritance with different eye color mutations and pinpoint where they occur in the pigment pathway. The drosophila eye color is a result of two bio-synthetic eye color pathways‚ this is expressed as a trait or phenotype‚ a multigene
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