unknown compound. In this experiment‚ chromatography and titration were performed to identify the unknown amino acid. Within experimental error‚ the results were consistent with the reference literature cited in this report. Experimental Thin Layer Chromatography The amino acid standards used in this experiment were Alanine‚ Glycine‚ Serine‚ and Histidine. These standards and the unknown were separated by performing a method of chromatography. Thin layer chromatography (TLC) was performed by using a mobile
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Fermented foods are made by the partial oxidation of sugars‚ or sometimes other organic molecules‚ in a food product. The difference between this and the normal cellular respiration is that the electron donor from the electron transport chain is an organic compound rather than oxygen. One challenge for producing fermented foods is the pH levels of the food. Bacteria have different optimal pH levels. Therefore‚ as pH changes due to fermentation different bacteria will begin to ferment the food. For example
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Size Exclusion Chromatography Size Exclusion Chromatography (SEC) is the separation technique based on the molecular size of the components. Size exclusion chromatography is a kind of method to separate different size of molecules that put in solution. It was first discovered by two scientists who named Grant Henry Lathe and Colin R Ruthven. Both of them received the John Scott Award for this fabulous invention. There are various applications for Size exclusion chromatography such as biochemical
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CHM 510 LABORATORY REPORT Experiment 1: Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature Name: AFIQ B. ANWAR Student No.: 2012621072 (AS2253A) Date of experiment: Date of report submission: Lecturer’s Name: PN. HALIZA Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature INTRODUCTION The main purpose of the experiment is to investigate the effects of column temperature and flow rate on the separation of methyl esters compounds
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Colouration of Silk with Natural Dyes Dr. Rajashree Phukon‚ Registration ID- AB/565 ‚ Assist. Prof.‚ Sibsagar Girls’ College‚ P.O.Sivasagar‚ Assam‚ Pin-785640‚ e-mail ID:rajashreephukon@yahoo.com and Mr Diganta Kr. Borah‚ Research Scholar‚ Department of Economics‚ Dibrugarh University‚ Assam‚ India‚ e-mail ID: digoborah@yahoo.in. ABSTRACT The study was undertaken with an aim to develop the dyeing conditions of four different natural dyes which are easily available
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Food Test 1: Test for Glucose – with Benedicts solution Benedicts solution is used to test for simple sugars‚ such as glucose. It is a clear blue solution of sodium and copper salts. In presence of simple sugars‚ the blue solution changes color to either green‚ yellow or brick-red‚ depending on the amount of sugar. Method 1) Mix smalls amount of each food sample (i.e.‚ Egg lumen‚ cylindrical piece of potato tuber‚ bread crump and crisps) in different test tubes with distilled water to make a
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SINGAPORE Chemical Engineering Process Laboratory II Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification
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Skin Dye made the dye bath turn into a light yellow color; However‚ this color did not look like the color of the onion skin. The Carrot Tops Dye had not changed the color of the dyed bath but stayed clear throughout the experiment. On the other hand‚ in Picture 2‚ the Zinger tea and Chamomile tea had deeper color in their dyed baths: orange and dark red‚ respectively. None of the yarn samples were the same color as the dyed baths‚ except for the yarn sample that was dyed with the onion dye. As shown
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Gas chromatography (GC) is a chromatography technique where the separation of individual components (analytes) from a sample relies on their differing distribution between a mobile and stationary phase. The mobile phase carries the analytes through the stationary phase. In GC‚ it’s an inert gas (usually helium or nitrogen). The gas must be inert‚ so it won’t react with the sample to give a false reading. The stationary phase is a substance fixed in place to which the sample adsorbs because
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test can be done for testing these sugar to show or prove are they reducing sugar or non reducing sugar.Example of the test that can be done is Benedict test. 1 In plant‚glucose is stored as the polysaccharide starch.Example of food that rich in starch is oat‚cereal‚rice and corn.Starch can be divided into two groups which is that is amylose and amylopectin. Natural starches are mixtures of amylose
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