LAB REPORT NUMBER TWO DATE: 3/25/2010 inal attachment Lab Experiment number 11 PURPOSE: To learn the Gram stain technique‚ the reason for the stain‚ and how to identify the results of the organisms stained. MATERIALS: Bunsen burner‚ inoculating loop‚ staining tray‚ glass slides‚ bibulous paper‚ lens paper‚ oil‚ and microscope METHODS: Apply Crystal Violet (Primary stain) for 1 minute. Rinse with D-water Apply Iodine (Mordant) for 1 minute. Rinse with D-water. Apply Alcohol (Decolorize) for
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seen at the top of the solution. Lead Acetate Cloudy solution with small specks of white precipitate. Ice cold ethanol Cloudy at the bottom and about 0.5 cm of the top of the solution was clear. Table 4: The results of Gel Filtration Chromatography Eluate Component Volume collected in mL First Blue dextrin 3.00 Second Brown cytochrome 7.00 Third Clear phosphate buffer 5.00 Table 5: The result of Electrophoresis of Amino Acids Distance
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LAB 4 Read over the parts of the microscope and answer the following questions: 1. What do you call the lens you look through on the microscope? Ocular 2. What is the difference between the ocular lens and the objective lens? Ocular lens is the lens you look through and objective lens is the lens that is close to the stage. 3. Where do you place the slide on the microscope? the stage under the stage clips 4. Which adjustment‚ course or fine‚ do you use when you are observing the
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FOOD CHAINS and FOOD WEBS There are two types of feeding relationships: autotrophic and heterotrophic. Autotrophic organisms make their own food by the process of photosynthesis e.g. plants. Heterotrophic organisms cannot make their own food (i.e. cannot carry out photosynthesis)‚ therefore must eat other organisms to get their nutrients e.g. animals. A food chain is the feeding relationship among organisms or the series of living organisms that eat each other. A food chain shows the transfer
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Why There Should be a Limit on Food Additives For many centuries people have been adding things to preserve foods. An example of this is using salt to preserve meats or adding herbs to enhance the flavor of any other type of food. In recent years‚ however‚ the amount of additives in food has dramatically increased‚ and some of the items that are being added have been questioned by scientists as well as some consumers. Some additives have been known to cause sickness and contribute to the formation
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junk food contains very high fat and sugar. . Also junk food has preservatives‚ chemicals‚ artificial colours and flavours. But healthy food is not containing these substances. However‚ both junk food and healthy food are delicious but junk food has more delicious than healthy food. The price is compatible with the pocket money for most of the kids and the teenagers who frequent the joints and it also is a great buy to makeup for the shortage of time. It is often quicker to eat or prepare than healthy
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binds the nucleoside inhibitor site. When it binds this site‚ it stabilizes the inactive T state and blocks the catalytic site which needs to be open for enzyme activity to occur. The glycogen phosphorylase b was purified with hydrophobic column chromatography and the concentration was determined with a Bradford Assay. The kinetics of glycogen phosphorylase b were studied by finding a molar extinction coefficient‚ 0.1617 mM cm-1 A-1‚ for
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REACTION PAPER As years go by‚ population rises‚ not just here in the Philippines but also in other regions of the different continents. And when the number of people multiplies so does their needs when it comes to providing food in their tables. Of course‚ for a very long time the Global Food and Beverage Sector has become part of the everyday lives of each person. No one can say that they can live without what can be provided by the said sector because most of us find profit in the jobs that
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IB SL Biology Lab Molecular Biology: Transformation and Electrophoresis Christina Qi 2/16/07 Aim: How can a plasmid be engineered to include a foreign piece of DNA and how does gel electrophoresis separate DNA molecules present in a mixture? Hypothesis: If the pGLO plasmid is inserted into competent Escherichia coli cells‚ then the transformed bacteria will be resistant to ampicillin and will glow green under UV light. If samples of DNA are cut using certain restriction
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Fast Food and Healthy Food Life today is not like how it was before. Along time ago‚ people ate foods that were field with balanced amount of nutrients value. Not with chemicals‚ preservatives‚ artificial colors and flavors which are known as Fast Foods. Long days of working and training effects our choices for food‚ a meal from nearest fast food restaurant becomes most appealing idea. Most of people who eat junk food more frequent don’t know its bad effect on their body which can be
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