Experiment 2 Titles Extraction of Bacterial Plasmid DNA and Analysis of Extracted DNA Samples Objectives * To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results
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DNA- is the material found in the nucleus that contains the genetic info. 4 billion codes in length Gene- a segment of DNA that controls a certain protein production. A gene is made up of hundreds to thousands of codes. Chromosomes- genetic material that is coiled up into structures during cell division. Importance of DNA- genes control the production of proteins..(look like‚ body function‚ body communications‚ and enzyme control) DNA screening- the process of testing individuals to determine
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strands of DNA double helix are separated‚ each can serve as a template for the replication of a new complementary strand‚ producing two daughter molecules each of which contains two DNA strands with an antiparallel orientation. The enzymes involved in DNA replication process are template-directed polymerases that can synthesize the complementary sequence of each strand with extraordinary fidelity. This complex leads to the local denaturation and unwinding of an adjacent A + T rich region of DNA. The interaction
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DNA Extraction from Fruit 1. What was the purpose of adding liquid soap and salt in step #1 and how does NaCl contribute to maximum DNA extraction. The purpose of using soap was to destroy the membranes inside a kiwi cell. Soap helped with that because it dissolves the membranes easily. Salt or NaCl was used to remove proteins and carbohydrates. NaCl caused the proteins and carbohydrates to precipitate. 2. Why was it necessary to “mush” the kiwi by hand? If the step was omitted‚ what
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DNA and Its Wonders (Each Title corresponds with a picture on the Glog) DNA in Society Genetic Testing – Genetic Testing allows the diagnosis of how vulnerable an individual is to inherit the disease. The results of a genetic test can confirm a suspected genetic condition or help determine a person’s chance to develop or pass on a genetic disorder. Being able to have this information is a great preliminary defense. Crime Scene Investigation – As the world progresses with new innovations we
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The Discovery of the Structure of DNA Determining the structure for DNA was one of the outstanding science achievements of the 20th century. It started in the late 1940’s‚ when scientists were aware that DNA was most likely the molecule of life‚ even though many were sceptical since it was so "simple." They also knew that DNA included different amounts of the four bases: adenine‚ thymine‚ guanine and cytosine (usually abbreviated A‚ T‚ G and C)‚ but nobody knew what the molecule might look like
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Comparison chart DNA RNA Stands for: DeoxyriboNucleicAcid RiboNucleicAcid Definition: A nucleic acid that contains the genetic instructions used in the development and functioning of all modern living organisms (scientists believe that RNA may have been the main genetic material in primitive life forms). A single-stranded chain of alternating phosphate and ribose units with the bases Adenine‚ Guanine‚ Cytosine‚ and Uracil bonded to the ribose. RNA molecules are involved in protein synthesis
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Extraction of DNA from Cheek Cells Gene Smith February 29th 2013 INTRODUCTION DNA‚ deoxyribonucleic acid‚ is the genetic material of every living organism and is found in the nucleus of eukaryotic cells. DNA is often called the ‘blueprint for life’ because it contains the necessary information to carry out all the living processes of the cell (1).The purpose of this lab was to extract DNA from human cheek cells. The isolated DNA could be used inmapping or sequencing‚ PCR‚ crime scene investigation
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Scientist extracts DNA to show how it’s useful to solve a crime scene. DNA is the most things to use for solving any crime. Finding DNA is pretty easy to find it can be you’re hair‚ blood‚ or even saliva. Using all that can help investigators to find who the criminal is. Scientist compares the DNA to make sure that their looking for the right person and not to make any mistakes. Scientists extract the DNA when they use blood‚ saliva‚ and other body fluids and tissues. For example the crime investigators
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Conclusions of DH5α DNA transformation with red colonies resistance to ampicillin and the lacZ gene Introduction: In this experiment‚ a plasmid with a gene that has resistance to the antibiotic ampicillin and has lacZ is used to transfer the resistance into E. coli bacteria in red colonies. This same technique is used to give diabetics their insulin‚ and to give dwarfs growth hormones. The point of this lab is to give the groups an idea how DNA can be transformed
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