this mutation a single nucleotide polymorphism changes a cytosine into a thymine that then can be detected using the polymerase chain reaction technique (Biology 225 lab manual‚ S2017). In this experiment amplification Refractory Mutation System PCR was used to detect this nucleotide change. Two primers were used in two different PCR reactions‚ one to detect the wild type allele and the other to detect the mutant allele. These primers are allele specific so the mutant type primer has the reverse complement
Premium Glucose Carbohydrate Sugar
ERIC-PCR technique is a quick and cost effective method for determining individual strains via demonstration of strain specific fingerprint bands. Therefore‚ the ERIC-PCR technique was used to determine the isolated strains of E.coli from different animal stool specimens. Material and Methods: The animal stool samples from hens‚ sheep and cows were obtained during 1 year. After screening processes‚ the E.coli bacteria were isolated and cultured using standard microbiological methods. The DNA
Premium DNA Polymerase chain reaction Molecular biology
------------------------------------------------- SIMULATION REPORT The objective of the project was to trade on a Market Simulation “STOCK TRACK”‚ from 2/13 to 4/20. We were supposed to try to trade different types of instruments in order to gain more knowledge about they are trade in the real world; and how a strategy which seems good can be overturned by the market circumstances. During this period as a conservative‚ I tried to trade less risky instruments. The qualities that I used to
Premium Stock Option Financial ratio
Lab 6: Isolation of Chromosomal DNA Mic 428L/ Section 001 Introduction: In biological research to address and eventually answer a multitude of questions‚ usually involves isolating chromosomal DNA. The purpose in this particular lab was to isolate chromosomal DNA from mutants grown and observed in lab 5 and then digest the DNA using a restriction enzyme. The fragments left from digestion will be ligated and then transformed into a strain of E. Coli DH5αλpir containing the pir gene pi product
Premium DNA Bacteria Molecular biology
The purpose of the DNA extraction lab was not only to inform students on how DNA is present in humans and all organisms‚ but to also educate them on how DNA can be extracted using common household materials. Also‚ the lab was very efficient as it introduced the students to extracting their own DNA found on their cheek cells as well as letting them take an observation on how DNA appears or how it is formed. Additionally‚ students were instructed through a very clear and simple procedure‚ which guided
Premium Education Learning DNA
Conclusions of DH5α DNA transformation with red colonies resistance to ampicillin and the lacZ gene Introduction: In this experiment‚ a plasmid with a gene that has resistance to the antibiotic ampicillin and has lacZ is used to transfer the resistance into E. coli bacteria in red colonies. This same technique is used to give diabetics their insulin‚ and to give dwarfs growth hormones. The point of this lab is to give the groups an idea how DNA can be transformed
Premium Bacteria DNA Gene
PTC Testing Lab 11/12/13 Abstract: The main purpose of this lab is to determine that you have the dominant PTC gene or recessive PTC gene. PTC testing is a method used to test for a genetic trait. People who have dominant gene taste PTC (phenylthiocarbamide)‚ and people who have recessive do not taste PTC. This trait is passed genetically from parents to their children‚ so that if a person has the trait‚ then at least one of their parents had the trait as well (New York
Premium Chemistry Experiment Science
DNA Lab Report SungYong Jang What is DNA? What do the letters stand for? What is it composed of? Where is it found? What is it shaped like? Answer in full sentences. It is the genetic material that can be passed on from parent to offspring DNA --> Deoxyribonucleic acid It composed of Deoxyribose (5 carbon sugar)‚ a phosphate and a Nitrogonous base (the 4 N-bases are Guanine‚ Cytosine‚ Adenine and Thymine) It is a double helix (looks like
Premium DNA
precise sort of DNA testing known as PCR-enhanced DNA testing on the gloves from victim’s car. PCR-enhanced DNA testing was done on semen stains found on the victim’s clothes‚ and the outcomes demonstrated that the specimen did not originate from Yarris‚ but it came from two unknown guys. PCR-enhanced DNA testing was then done on scrapings found under the casualty’s fingernails‚ which uncovered that the scrapings were from the "regular user" of the gloves. He was released in 2004 after DNA prove at long
Premium Crime Criminal law Police
Forensic DNA testing In Today’s forensic DNA testing is used all around the world to catch criminals. Forensic DNA testing is able to catch suspects and analyze their data correctly to be able to identify their suspect and be sure to identify their suspect and be sure it is the right person. DNA testing is 100% accurate and is the most effective type of testing there is and can change many lives such as criminals‚ fathers and children. Before forensic DNA testing
Premium DNA Crime DNA profiling