Abstract This article is investing the effects of speed of the action potential across many neurons through investigating two diseases and performing related lab simulations. Multiple sclerosis and epilepsy are the two disease which are investigated and through the use of Neurons in Action lab simulations‚ we saw the effects that demyelination and channelopathy can have. As my hypothesis guessed‚ demyelination is the main cause of multiple sclerosis and channelopathy is the main cause of epilepsy
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isolated. Isolating DNA from the Pure Colonies Grown In addition‚ DNA were isolated from the colonies grown and its concentration was 33.6 ng/uL and the A260/280 value was 1.84. Next‚ an agarose gel electrophoresis was conducted to see if any DNA fragments were present. A band was visible and that validates that genomic DNA has been isolated. PCR Amplification to Determine Strain of P. larvae with ERIC-Specific Primers ERIC-specific primers were used to genotype the genomic DNA of P. larvae isolated
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procedure was taken from “From Drosophila cDNA in E. coli plasmid to homologous human proteins” lab manual (4). - Colony Picking: Two E. coli colonies were grown on agar plates and treated with ampicillin. They contained the plasmid with genes for ampicillin resistance and Drosophila cDNA sequence. - Plasmid Isolation: We used the QuickLyse Miniprep Plasmid DNA purification systems to isolate the plasmid DNA. Indeed‚ the bacterial cells were removed from the liquid broth and were resuspended in the lysis
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Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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Title of Report in Initial Capital Letters: Times New Roman (24 point‚ Boldface) and No more Than Three Lines Your Name Name of Your Department Southern Polytechnic State University Date Title of Report in Initial Capital Letters: Times New Roman (18 points‚ Boldface) and No More Than Three Lines Your Name Name of Your Department Southern Polytechnic State University Date Summary All reports should include a one- or two-paragraph summary. This summary
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INTRODUCTION This report is based on the business game we played on footwear industry. Whilst the task set for this group business game is strategic in nature and obviously requires the team‚ a variety of strategical knowledge. It is my experience that there are several other areas of expertise equally important. A large amount of time and effort throughout this game has been spent on team-working - organising the team‚ processing and collating the vast amount of information that it created‚
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ILLINOIS STATE POLICE Division of Forensic Services Education Forensic Science Laboratory ISP CENTRAL HEADQUARTERS * 400 N Broadway Ave URBANA‚ Illinois 61820-0 (217) 384-3888 (Voice) * 1-(800) 255-3323 (TDD) Pat Quinn Hiram Grau Governor Director April 18‚ 2014 LABORATORY REPORT DET. Stabler CHAMPAIGN POLICE DEPARTMENT 82 E UNIVERSITY AVENUE CHAMPAIGN IL 61820
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Contents REAL TIME PCR 2 REAL-TIME QUANTITATIVE PCR (qPCR) 2 BASIC PRINCIPLE 3 TYPES OF PCR 4 qPCR STEPS 4 ONE-STEP OR TWO-STEP REACTION 6 Overview of qPCR and qRT-PCR components 6 REAL TIME PCR SYSTEM: 7 SOFTWARES FOR DATA ANALYSIS AND PRIMER DESIGNING 8 STANDARD REAL-TIME PCR PROTOCOL 9 ASSAY DESIGN 9 2. Nucleic acid purification 9 3. Reverse transcription 9 4. Controls and normalization 9 5. Standard curve evaluation of efficiency‚ sensitivity‚ and reproducibility
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Pseudomonas taiwanensis – Enrichment‚ Isolation and Identification Microbiology 521 2/10/12 Purpose: The purpose of this experiment was to enrich Pseudomonas bacteria‚ isolating a species of Pseudomonas and identifying it using phenotypic properties and DNA sequencing as an existent or completely new and undiscovered species of Pseudomonas. Overview: Genus Pseudomonas is a chemoheterotrophic bacteria found in soil and water. They are Gram negative‚ motile‚ paired rods that are also oxidase-positive
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Brandon Schmetterer 3-13-15 Biology labs DNA Extraction Lab DNA is extracted from humans for genetic testing‚ for body identification‚ and for analysis of forensic evidence. The first step of DNA extraction is to take cheek cells from the test subject. Next‚ the cells must be burst open in order to release DNA. Third‚ DNA is separated from protein and debris. Lastly‚ the DNA must be isolated. A buccal swab is necessary in order to collect the cheek cells .The micropipettes are used to add lysis
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