Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction sites generally
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Introduction: Through a series of labs‚ we were able to extract and isolate our mitochondrial DNA‚ nuclear DNA‚ and cellular DNA to then amplify a part of an intron in the tissue plasminogen activator gene (TPA) through Polymerase Chain Reaction (PCR) and run on an agarose gel to detect the presence or absence of a transposable element known as an Alu element. The main purpose of this experiment was to use our class as a population‚ to test whether or not we were in Hardy-Weinberg Equilibrium. The
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Introduction The Everest group simulation was an online simulation which encouraged five students to play a unique and vital role on a team of hikers. The main goal is to reach the summit of Mount Everest. The simulation stimulates practical application of concepts and theories managerial decisions‚ especially in the form of groups. The purpose of this report is to reflect on the experiences encountered in the team during the simulation and identify relation of our experience and management
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Biology 160 Lab Manual Tacoma Community College Last update 06/03/2013 This page left intentionally blank Table of Contents Table of Contents i Laboratory 1: Principles of the Scientific Method 4 In-Lab Report Sheet 12 This page has been left intentionally blank 27 Laboratory 2: Scientific Methodology & Enzyme Activity 28 In-Lab Report Sheet 28 Objectives 33 Introduction 33 Parts of the Swift M5 Microscope 33
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CCSI 410 Forensic Lab Report 1) Investigator’s Name: 2) Date of Investigation: August 2‚ 2014 3) Lab Number and Title: Lab 4 Keyword Searches 4) Summary of Findings: I did the steps required to fulfill my report. I found there is enough evidence to continue the investigation due to the search results. 5) Details of Investigation 1. 11.45 pm – Turned on suspect computer 2. 11: 47 pm – Entered lab environment 3. 11: 49 pm – Determined keyword list 4. 11:59 pm – Added
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Strawberry DNA Extraction Donovan Roberts Mrs. Caudill Honors Biology 3/7/11 Introduction The structure of DNA is made of nitrogenous bases‚ phosphate groups‚ and sugars. These three parts of DNA form hydrogen bonds and create a right-handed double helix. The location of DNA in a eukaryotic cell is found within the nucleus‚ bound to proteins. DNA can be extracted from within the cell. They are several steps you must take to retrieve DNA from within the cell. When extracting DNA cells can
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DNA typing was first used in Great Britain for law enforcement purposes in the mid- 1980s. It wasn’t employed in the United States until 1987. DNA profiling has changed forensic science. DNA technology has given police and the courts a means of identifying the suspects of rapes and murders. Today‚ the Federal Bureau of Investigation performs the bulk of the forensic DNA typing for local and state law enforcement agencies. In criminal investigations‚ DNA from samples of hair‚ bodily fluids or skin
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1. The overall goal of this lab was to carry out experiments that clearly demonstrate the different ways DNA genetic information‚ specifically transduction and conjugation. The first half of this experiment focused on exploring the mechanisms of transduction. This was done by creating a spot titer plate for phage carrying kanamycin resistance and E. coli. E. coli was then proven to have gained kanamycin resistance throughout transduction as demonstrated by its ability to grow on a medium containing
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science plays an extreme role. DNA is the abbreviation for Deoxyribonucleic acid. It is present in all organisms‚ whether it is a bacteria‚ bird or mammal. DNA is a long molecule that carries a particular genetic data; it is located in every cell in a human body except the red blood cells. DNA profiling is easy acquire and very accurate in identifying a person. There are many different ways a forensic expert or a police officer can get DNA samples. In criminal cases‚ DNA samples help solve many crimes
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precipitate the cellular components. The mixture was then centrifuged for 10 minutes and the supernatant was transferred to a new tube. Isopropanol was then added to precipitate the plasmid DNA and centrifuged into a pellet‚ dried‚ and then resuspended with distilled water. This plasmid DNA was then run on an agarose gel to view the stands of
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