Proteomics is the study of proteomes‚ which are groups of proteins in living systems. It is used to examine when and where proteins are being expressed‚ rate of degradation‚ modifications‚ how they interact with other proteins and more. The goal in this lab was to determine the similarities and differences in muscle proteins among 5 species of fish using SDS-PAGE and Western blotting. First‚ proteins are separated based on size. Second‚ antibodies are used to detect the protein of interest. Lastly‚ a substrate
Premium Protein DNA Gene
Unit 1 Lab Questions: Jeannie Lozowski Hair Detective 1. Describe the crime that you are investigating in this activity. In this activity‚ we had to discover who stole the rock bands hair products. While the band was in rehearsal‚ someone snuck into their dressing room and stole it. The trail of hair gel lead to the room of another girl band. 2. What evidence do you have to identify the culprit of the crime? We have strands of hair of the culprit and other band members. We tested the hair
Premium Alcoholism The Band Hairstyle
Introduction A sample of DNA found in a crime scene was provided along with five suspects. Their DNA was then processed using restriction enzymes and Agarose Gel Electrophoresis. The objective of this lab was to match a criminals DNA to a crime scene using restriction enzymes EcoRI and Pstl with Agarose gel electrophoresis. Restriction enzymes cut DNA at a specific base pair site recognized by the enzyme‚ which then turns one single strand of DNA into many fragmented strands of DNA. EcoRI recognizes
Premium Molecular biology DNA Genetics
Isolation of Recombinant Taq Polymerase for PCR Isolation of Recombinant Escherichia coli IPTG induced Taq polymerase and characterization through polymerase chain reactions‚ Western Blotting and gel electrophoresis * Braeden Cowbrough1‚ Michael Atkins2‚ Christopher Bonner3 From the Faculty of Biochemistry Lab 3006 B Carleton University‚ Ottawa‚ ON K1S 5B6 *Running title: Isolation of Recombinant Taq Polymerase for PCR To whom correspondence should be addressed: Braeden Cowbrough‚ Faculty of Biochemistry
Premium Polymerase chain reaction Molecular biology
2520 2500 2000 *light blue is 1 band Experiment The group first designed the materials to create and hold the .75 agarose gel and experiment. Everything except the comb was made of ¼ inch Acrylic Plexiglass. The glass helped the group see what was going on in the experiment. The comb was 3D printed to be more accurate. One material was a casting tray which held the Gel in future steps. The casting tray had to hold at least 50 mL of liquid. They didn’t want the casting tray to hold much more
Premium Electric charge Molecular biology Electric current
BIO 219 Group 1 Section 66 October 19‚ 2012 The extraction of purified DNA from A. fischeri by restriction digestion using Sal I enzyme and pGEM for shotgun cloning Introduction: The ultimate goal of this experiment is to isolate the lux operon‚ a targeted piece of DNA that causes bioluminescence‚ from Aliivibrio fischeri and insert it into the DNA of Escherichia coli in order to make it glow. A. fischeri is a gram-negative bacteria which participates in a symbiotic relationship with
Premium DNA Escherichia coli Bacteria
The purpose of this lab is to implement the technique of gel electrophoresis in the purification and size determination of various proteins and DNA fragments. In order to do this‚ a polyacrylamide gel will be prepared and placed in a buffer-containing gel electrophoresis apparatus. Next‚ an aliquot of acid phosphatase and a molecular weight marker (Composed of Phosphorylase B‚ bovine serum albumin‚ ovalbumin‚ and carbonic anhydrase) will be placed into separate wells within the gel‚ and the apparatus
Premium Gel electrophoresis Molecular biology DNA
very useful because of its ability to detect a specific DNA sequence from a large amount of DNA ⎯ even from the whole genome. This specific sequence can be found through a combination of two different techniques: Agarose Gel Electrophoresis and Hybridization‚ which is known as Southern Blotting. This technique if performed in three phases: (I) prepare the gel and (II) make the blot‚ (III) hybridize and visualize blot. In phase one‚ the gel is prepared in three steps: (1) chemical digestion‚ (2)
Premium DNA Molecular biology Water
Background on Genomic DNA Isolation and Purification Generally‚ all methods involve the disruption and lysis of cells. This is followed sometimes by the removal of RNA (by RNAses‚ salt or other methods). Choosing which method to use will depend on many selection factors including: DNA is isolated from proteins by several methods including digestion of proteins by the enzyme proteinase K. Proteins are removed subsequently by salting-out‚ organic extraction‚ or binding of the DNA to a solid-phase support
Premium DNA Molecular biology
Your first lab link can be found at Case One: Rookie Training. 1. What are the four basic steps of DNA processing? The four basic steps of DNA processing are extract‚ amplify‚ separate‚ and analyze. 2. From the toxicology lab‚ what is vitreous humor? Why do forensic scientists use it? Vitreous humor is the clear liquid inside the eye that gives the eye its shape‚ forensic scientists use this to confirm results received from the blood or urine. 3. From the survey in the toxicology lab‚ would you
Free Firearm Gun Rifle