Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained‚ with elution 3 containing the most amount
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Institute Physics Course 2014 - Rocket Lab Report Title: RockSim and Electronic Altimeter Measurement Techniques Name: Scarlet Henriquez Email: scarletpatricia94@aol.com LAB SESSION ___8____Assignment _____7_____ Due Date: ___7/8/14______ Objective: Launch a smaller rocket to learn how to compare RockSim predictions (computer based) to an actual instrument measurement carried by the rocket (Electronic Altimeter). This Lab shows how better the electronic measurement
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Forensic DNA Evidence DNA has become one of the most accurate tools used in law enforcement in determining guilt or innocence. DNA is different in all people it is our “genetic blueprint.” DNA is so significant to law enforcement because DNA left at a crime scene can be collected and tested to see if there is a match. It is unique because it ensures accuracy and fairness. The initial use of DNA began in Britain in 1986; the FBI used it for the first time in the United States two years
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Forensic biology is the application of biology to law enforcement.It includes the subdisciplines of Forensic anthropology‚ Forensic botany‚ Forensic entomology‚ Forensic odontology and various DNA or protein based techniques. Applications Forensic biology has been used to prove a suspect was at a crime scene‚ identify illegal products from endangered species solve crimes by matching crime scene evidence to suspects‚ investigate airplane bird strikes‚ and investigate bird collisions with wind turbines
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Biology January 21‚ 2013 Forensic Science In today’s age of technology‚ there are many advanced techniques detectives use to get to the root of a crime. Forensic anthropology and other sciences are key to finding the true cause of a person’s death. Without forensics‚ solving crimes would be very difficult because of the lack of evidence from various sources. Some biologists are specially trained and educated in molecular biology and can study a person’s DNA (Ferlini 17). Identifying the
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DNA Profiling and Ethics Reflection Journal Vanshika Khemka 14th October 2012 "33 autorad off" On 10th September 1984‚ geneticist Alec Jeffrey’s wrote these three words in his red desk diary. This marked the completion of an experiment‚ which studied how inherited illnesses pass through families. The experiment failed entirely. (McKie‚ 2009) However‚ this led to the most profound discovery: the world’s first DNA fingerprint. Now‚ the smallest swab of blood or sweat can determine
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CHARACTERIZATION BY ELECTROPHORESIS Abstract The molecular weights of protein extracts were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two sets of four protein samples‚ standard bovine serum albumin (BSA)‚ invertase‚ egg albumin‚ and casein‚ were prepared; one set containing β-mercaptoethanol (BME) while the other did not. These were then analyzed through SDS-PAGE with 12.5% resolving gel‚ prepared using 2 M Tris-HCl at pH 8.8 and stacking gel‚ prepared using
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determine the unknown DNA plasmid using restriction enzymes and conducting electrophoresis finally comparing the resulting fragments with the known restriction map. In this lab‚ it succeeds in showing the fragments. In this report we will discuss the‚ results‚ limitations and possible errors. Introduction In biology restriction enzymes are used in several ways to modify and manipulate DNA molecules. One common use is to compare pieces of DNA from one that is unknown‚ with fragments of DNA from another source
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Lipid and protein composition varies in tissue types. Results: When comparing liver‚ muscle‚ and adipose tissue‚ adipose tissue had the highest percent lipid and liver tissue had the highest percent protein. Conclusion: Based on analysis of the gels‚ the liver appeared to have the most variety in proteins when comparing liver‚ muscle‚ and adipose tissue. Significance: In order to determine the composition of a tissue‚ specific macromolecules can be extracted‚ quantified‚ and analyzed. ABSTRACT
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FORENSICS Forensics‚ by and large‚ is the application of science to the legal process. It is an emerging research domain in India. There are many different types of forensic sciences baring their vital presence possibly in every field of human endeavor. Of these‚ let us now discuss about the computational‚ cyber and the DNA forensics. COMPUTATIONAL FORENSICS: The development of computational methods or mathematical and software techniques to solve forensic issues is called computational forensics
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