Protein (rGFP) from the E.coli strain using Ni2+ agarose affinity chromatography technology Abstract The purpose of this experiment was to express and purify the his6-tagged recombinant form of GFP (rGFP) from the organism E.coli using Ni2+ agarose affinity chromatography. The expression of rGFP was confirmed qualitatively using the UV light and was expressed in the E.coli strain BL21 (DE3) (-- removed HTML --) (-- removed HTML --) . Ni2+ agarose affinity chromatography was used to purify the crude
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I.Purpose The purpose of this lab is to show how potential energy and kinetic energy is shown and transferred using a model rollercoaster. This lab also demonstrates the Law of Conservation of Energy. II.Introduction Potential and Kinetic energy have a very big relationship. The Law of Conservation of Energy states that “Energy cannot be destroyed or created‚ but can be transformed or transferred.” This lab will help demonstrate this law and show the conversion between Kinetic and Potential
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McLean 1 Creating Acetylene Gas Introduction The purpose of the lab was to determine the ratio of air to acetylene results in complete combustion of acetylene gas. The balanced chemical equation for this experiment was C2H2(g) + O2(g) --> CO2(g)+ H2O(l). Complete combustion is the reaction of an element or compound with oxygen to produce the most common oxides and energy. Complete combustion occurs when the fuel and oxygen combine in exact proportions to completely burn the fuel
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Forensic Science lecture 4 – 17/01/2013 Quiz: quiz after every 2 weeks‚ done through bb‚ posted midnight Sunday 12‚ close midnight Monday at 12. Video watched through class‚ Guest speaker: Heather Shacker – forensic biologist * biology section: identify of body fluids: blood‚ semen saliva‚ and DNA analysis * casework approach: find material‚ identify‚ analyse (DNA)‚ evaluate/compare (after able to create DNA profile)‚then lastly interpret * how does that work? 3 main groups of DNA
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One of the main learning experiences of this lab is to learn the importance of calibrating a sensor. In most cases‚ a sensor will not have an output that is exactly what you need. An example of this is a strain gage. A stain gage gives back a voltage‚ but with that given voltage a distance can be found. Introduction: In this experiment‚ the QNET-MECHKIT was used. This board has multiple compact sensors that can be used for experiments. For this lab we will be using the strain gage to find the
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It was hypothesized that if the animal was larger‚ then it would have larger hemoglobin. It was thought that larger mammals would need more oxygen-rich hemoglobin to support healthy function. This lab did not support this hypothesis. The results in table 1 displayed that the hemoglobin of all mammalian samples traveled about the same distance. This trend can be seen in graph 1 as the mammalian hemoglobin samples migrated the same distance. Because the proteins traveled around the same distance it
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Gene Cloning Methodology of DNA What is DNA? DNA was discovered by the Swiss biochemist‚ Johann Friedrich Miescher‚ in 1869‚ while he was working in Tubingen‚ Germany. He found that the DNA molecule is large; acidic in nature and rich in phosphorus‚ but only in the 1930s was the real and complex structure of DNA fully studied. DNA (deoxyribonucleic acid) is the genetic material in all prokaryotes and eukaryotes‚ i.e. it is the material responsible for the transfer of hereditary traits from
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Report concerning the decisions and results of Period 2. 09.01.2013 Business simulation Executive Summary This report provides the Chairman of the board an analysis of the decisions and the results of the Company 1 in Period 2. The focus is given on how our company organized the process of planning in the Period 2 and I tried to critically analyze the results compared to our plans. In period 2 our company was seemed to do better than the previous period (period 1)‚ but in my opinion period
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Nervous System lab was designed to measure reaction times by using a variety of tests that affect the heart rate. These tests were the startle‚ stroop‚ Valsalva maneuver‚ diving reflex‚ and a test of our choice. The test our group chose to measure the heart rate in response to doing a trust fall to anticipate the stress right before the fall. To begin this lab we chose a participant‚ and then hooked their finger up to the pulse transducer sensor. Using the chart 5 program on the lab computer the participant’s
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PRE LAB REPORT Reynaldo Riboul TLC and Column Chromatography October 6‚ 2013 Table of Chemicals: Chemical Hazards Mol. Wt. Density Grams Moles Acetone Flammable‚ Irritant 58.08 g mol−1 0.791 g cm−3 2.0 g 0.0344 Hexane Flammable‚ Irritant‚ Dangerous to Environment 86.18 g mol−1 .6548 g mL−1 9.0 g 0.1044 Fluorene Very toxic to aquatic life with long lasting effects 166.223 g/mol 1.202 g/mL 0.3 g 0.00180 Fluorenone Irritant 180.20 g mol−1 1.13 g/cm3 0.3 g 0.00166
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