cell 2. Treat both w/ the same restriction enzyme * Recognition sequence cuts through lacZ gene in plasmid 3. Mix all DNA fragments with plasmids. * H-bonds bt/n bases temporarily hold “sticky ends” together 4. Add DNA ligase: creates permanent covalent bonds 5. Reintroduce plasmid to bacteria via transformation 6. I.D. recombinant cells by * Ability to grow in * Inability to digest x-gal(the colonies will be white‚ not blue) X-gal -------------------------------- (β galactosidase)--------------------->
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* Independent: * Colour * Rate of diffusion Controls: In this experiment the control is solution B with the room temperature water. Procedure: Step 1:Get 3 clear plastic finger bowls Step 2:Label the bowls with tape and with permanent black marker label them A‚B‚ and C. Step 3:Boil 1 cup of water in a pan on the stove on medium heat (5-7) until it reaches a rolling boil. Step 4:Put a cup of room temperature water in a freezer that is 90 degrees Celsius for 30 minutes. Step 5:Put
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9 grams of steel wool 2.9 grams of copper 2.9 grams of iron Masking tape Permanent marker Procedures 1. Fill all six jars with water so that they are nearly full. It may be helpful to use a measuring cup to make sure that each jar has exactly the same amount of water. 2. Attach a vertical strip of masking tape to the side of each of your test tubes (for marking the water level)and using the permanent marker‚ make a mark on the tape about 1 cm down from the mouth of the test tube. This will be the outside water level
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Methods Body Paragraph # 1: In the first experiment‚ Effects of Groundwater Contamination I used the following materials: (8) 250 ml Beakers|Permanent marker|3 Wooden stir sticks|100 ml Graduated cylinder|10 ml Vegetable oil|10 ml Vinegar|10 ml|Liquid laundry detergent|100 ml Beaker|240 ml Soil|Funnel|Cheesecloth|Scissors|Water. With a marker the eight 250mL beakers were labeled number one through eight. Putting five through eight to the side‚ fill with beakers one through four with
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This means verify that they have the correct person for the procedure‚ the type of procedure itself‚ and verify this information once more with the patient. Another strategy is marking the operative site with a permanent marker that could be visible enough after the skin has been prepped‚ this helps identify the surgeon which site needs to be operated. Taking the time out with all the team members before procedure allows verification of which site will be operated‚
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Students Is English your first language: No (please delete as applicable) STUDENT NAME: XOLILE SHABANGU STUDENT NO: 13083J NAME OF MARKER: Lisette Niemand DATE OF SUBMISSION: 01 March 2013 ASSIGNMENT NO: O A NO. OF WORDS: BIBLIOGRAPHY ENCLOSED: Yes (please delete as applicable) TASK ONE The foundation for the child ’s development is concentration
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Rotundo March 2014 The advance of medical science within the past two hundred years is nothing short of astounding. During the 1960s‚ the topic of improved disease treatment was initially introduced (Walters‚ 2012). The advent of the permanent integration of genetics within the ideology of bioethics came in 1972. A primary determinant in understanding the genetic aspects of human disease is the Human Genome Project‚ which has been taking tremendous strides toward progressions for diagnostic
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the three little wolves ran away from the pig and built a new house. This is similar to social situations where those who are left out usually end up being the predators in shootings that take place in schools or in any heavily populated area. The permanent labeling is not only common in bullying‚ but also in other situations. For example‚ if someone gets thrown into jail‚ the next time they try to find a job they will most likely be rejected because a criminal record of any sort is deemed unworthy
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Micro test tube • 10mm measuring cylinder • Stirring rod • Stop watch • Bunsen burner • Piece of Magnesium • Sand paper • Permanent marker 2.2 Diagram / Photograph 2.3 Procedure Firstly an aim and hypothesis were written up‚ then 2mm of water was poured into the measuring cylinder and that was tipped into the micro tube. Then The 2mm line was labelled with the permanent market. That water was then discarded. After this the piece of magnesium was cleaned with sandpaper and bent into shape. The
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cylinder of beetroot‚ labelled TT4. 4. Test tube rack 5. A bottle containing 8 ml distilled water 6. A bottle containing 8 ml 50% acetone 7. A bottle containing 8 ml 100% acetone 8. Permanent marker pen 9. 1x sheet graph paper Experimental procedure 1. Using the permanent marker pen‚ label the empty
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