University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Abstract Photosynthesis is a food making process for algae and plants. The photosynthesis process rate varies from different wavelengths and intensities of light. This lab will evaluate the optimal wavelengths and degrees of intensity during photosynthesis when chloroplast is exposed to light. The mixtures of DCPIP with water‚ PO4 buffer‚ and chloroplast will be prepared in a number of cuvettes. The cuvettes were tested individually at different wavelengths and intensities to find the optimal rate
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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Enzyme Catalysis Lab: Laboratory Write Up Problem/Question: What happens to the amounts of gas produced in a catalyzed reaction of Hydrogen Peroxide when the catalyst is mixed with an acid? Theory/Hypothesis: In this experiment the researcher and their team will be performing tests on a catalyst (proteins found in potato) to see what will happen when the catalyst in a Hydrogen Peroxide reaction is treated with an acid before the reaction occurs. The researcher hypothesizes that the after treating
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[Teacher] [Course] [Date] Catalase Lab Report An enzyme is something that helps to speed up a chemical reaction. The enzyme changes from reaction to reaction‚ but it always has the same impact. However‚ certain variables may cause the enzyme to have a more or less significant impact on the speed of each reaction. One of these variables that changes the effectiveness of an enzyme is temperature. There is an optimal functioning temperature for each enzyme in each reaction‚ depending on the desired
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BACK TITRATION- DETERMINATION OF THE CARBONATE CONTENT IN GARDEN LIME NAME: OSEI BONSU ERIC ID: 3906409 EXPERIMENT: I.2.2.1.
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is involved with the cannon being fired then the force of gravity would cause the cannon to start to slow down‚ wouldn’t go as far and the direction or trajectory of the cannon ball would be a curved line going down. Purpose: The purpose of this lab is to give the student a better understanding about projectile motion and to understand Newtons theories and laws of motion. We will be firing the plastic ball from the projectile launcher three times from eight different angles from the ground &
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Lab Report #1 Title: Synthesis of Divanillin Abstract: The purpose of this experiment was to synthesize divanillin. This was done via the oxidative dimerization of two equivalents of vanillin‚ using enzyme horseradish peroxidase as the catalyst. Procedure: Lab Handout: Nishimura‚ R.T.; Giammanco‚ C.H.; Vosburg‚ D.A. J. Chem Educ. 2010‚ 87‚ 526-527. Discussion: Mechanism: Reaction 2 Vanillin H2O2 Divanillin 2 H2O
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total of 15 points. To receive full credit for Exercise 8‚ you must submit the following: 1) The typed Exercise 8 report form‚ complete with all of your data recorded and questions answered. 2) A full scientific report for the Part I experiment that your group conducted. Use the supplemental guide to writing a scientific report to help you produce this report. Part 1: Does light matter? Table 8.1: Group and Class rates for Photosynthesis by Elodea sprigs. |Light Condition
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The Charge/Mass (e/m) Ratio of the Electron PHYS 0212: Introduction to Laboratory Physics Fall 2012 Abstract The experiment conducted demonstrated correlation between the charge and mass of an electron and the behavior of magnetic fields. The lab was divided into four parts. The first three parts were conducted with a compass that was used to locate the magnetic field lines around a bar magnet‚ a solenoid and then a pair of Helmholtz coils. As a result of these trials‚ our observations found
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