------------------------------------------------- Enzyme Pre-Lab Harry Kang 9/26/12 1) The purpose of this lab was to determine the rate of enzyme activity under variety of different conditions‚ such as‚ different amount of drops of enzymes and different temperature of water. The class measured the pressure in the test tube during the reaction of the substance with‚ 1.5 ml of H2O2‚ 1.5ml of H2O and different amounts of enzyme drops‚ to determine how much oxygen gas is produced during the reaction
Premium Gas Oxygen Pressure
Enzymes in Living Tissues Purpose: The purpose of the lab was to analyze the enzymes in living tissues‚ represented by the pieces of liver. Also‚ hydrogen peroxide was used to demonstrate these effects. Storyboard: Materials: 2 50 mL beakers 10 mL graduated cylinder 3% hydrogen peroxide solution Hot water bath Lemon juice or HCl Fresh liver Forceps Procedure: Measure 10 mL of hydrogen peroxide and record its temperature. Pick the liver up with the forceps and
Premium Water Hydrogen peroxide Chemical reaction
Title of Lab: Preparation and Assay of Phenolase and Peroxidase from Sweet and Irish Potato Hypothesis: Polyphenol Oxidase enzyme activity can be detected by change in colour of solution‚ Inhibitors prevent the reaction of the enzymes with substrates‚ the enzyme is relatively specific. Aim: To design and conduct an experiment to demonstrate enzyme activity of Polyphenol Oxidase and Peroxidase when mixed with catechol‚ caffeic acid‚ pyrogallad‚ tyrosine‚ guacol‚ and water‚ to test the effect of
Free Enzyme
Elizabeth Au (1) Serena Chan (3) Sharon Chan (4) Christy Chan (5) BIOLOGY LAB REPORT Investigation of the effect of metal ions on enzyme activity Objective To study the effect of two heavy metal ions‚ lead (II) ions and silver ions on the enzyme activity of invertase. Introduction Hypothesis: The rate of enzyme reaction of invertase decreases and the reaction eventually stops as the heavy metal ions inhibit the enzyme reactions. Biological principle: Heavy metal ions are non-competitive inhibitors
Premium Enzyme Silver
was designed to investigate the effects of temperature on reactions between the enzyme catalase found in animal tissue with the substrate H2O2. The hypothesis stated that an increase in the temperature of the substrate would create a subsequent increase in the rate of reaction between the enzyme and the substrate. This hypothesis was tested by immersing 1cm cubes of animal tissue (sheep liver) which contained the enzyme catalase into the substrate (H2O2 ) mixed with detergent which foamed showing a
Premium Measurement Catalase Chemical reaction
ENZYMES LABORATORY REPORT Introduction The utilization of any complex molecule for energy by an organism is dependent on a process called hydrolysis. Hydrolysis breaks complex molecules into simpler molecules using water. Similarly‚ the process that is the reverse of this is called dehydration synthesis‚ which removes water from simpler molecules. However‚ because hydrolysis occurs very slowly‚ living organisms use biochemical’s called enzymes to speed up the reaction. In this lab exercise
Premium Enzyme
Chavez Period 1‚ Bio Acc. Mrs. Petrov October 1‚ 2014 Tyrosinase Enzyme Lab Question What are enzymes and how can we cause changes in enzyme function? Objective The objective of this experiment is to observe if changes in temperature will cause a disruption in enzyme function. Hypothesis If we add and boil enzyme in L-Dopa‚ then the color will become darker because the temperature will denature some of the enzymes. Variables Independent: Temperature Dependent: Color change Control:
Premium Enzyme Chemistry Protein
Virtual Lab: Enzyme Controlled Reactions Worksheet 1. Which of the following does NOT apply to an enzyme: Inorganic 2. When an enzyme catalyzes a reaction: Substrate(s) bind in the active site 3. Which of the following would interfere most with the ability of an enzyme to catalyze a reaction? Reduced concentration of substrate available 4. Feedback mechanisms regulate the rate of enzyme activity‚ effectively “turning off” an enzyme in a
Premium Enzyme PH Chemical reaction
Enzyme Specificity Using Beta-galactosidase and Alpha-galactosidase Introduction: In order to survive‚ organisms need reactions to occur at a certain time and place. Enzymes make these reactions happen. Enzymes are highly specific causing only one enzyme to catalyze one reaction. “Induced fit” explains why enzymes act this certain way. An enzyme will surround a certain substrate and form to it for the reaction to occur in the active site. If it doesn’t form to it‚ the reaction will not occur
Premium Enzyme Glucose
Investigate Concentration of Substrate on Catalase enzyme 1. Aim/Prediction. Aim of this experiment is to investigate concentration of substrate on catalase enzyme‚ as well to see how they work in different concentrations of hydrogen peroxide (H2O2). Prediction would be‚ that the filter paper discs that were used‚ will rise up to the surface in a time in the tube of different concentration of hydrogen peroxide. And in different concentrations the time will be different. As higher the concentration
Premium Catalase Enzyme Hydrogen peroxide