Question One Description on what junket contains and how it works. “The dessert named junket is made by adding the enzyme Rennin to lukewarm milk. Rennin. also known as Chymosin or in its commercial form as Rennet‚ is found in the fourth stomach of cud-chewing animals‚ and is to be found in particularly high quantities in the fourth stomach of suckling calves. The enzyme curdles the milk by transforming caseinogen into
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Name: Fabiola Gonzales ID#: 810004692 Lab Partner: Onecia Alexander Date: Friday 23rd October 2015 Theory: Arginase is an enzyme with the E.C. number 3.5.3.1 (Worthington 2015). This details it as a hydrolase enzyme that catalyses the cleavage of bonds through the addition of water. For this experiment‚ we pay close attention to the reaction where arginase catalyses the hydrolysis of arginine to ornithine and urea. This reaction is a part of the urea cycle and occurs in mammalian livers and sometimes
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A Quantitative Enzyme Study: CATALASE FlowChart Purpose: Measure the rate of enzyme activity in different conditions Procedure: A. Extraction of Catalase 1. Peel potato 2. Cut into cubes 3. Mass 50g 4. Measure out 50ml of cold distilled water in blender 5. Add crushed ice into blender (small amount) 6. Add the potato cubes into the blender 7. Turn on blender on high for 30 seconds 8. Prepare an ice bath - FROM THIS POINT ON PREPERATION MUST BE CARRIED OUT IN AN ICE BATH – 9
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Enzymes are a type of protein. They are catalysts meaning they speed up the rate of the reaction. Enzymes activity depends on the concentration of the substrate‚ temperature and the pH. The more concentrated the substrate is the more reactive the enzyme is. The optimal pH for an enzyme is 7.5 and the optimal temperature for an enzyme is 53 Celsius. Extremes in the temperature and the pH of an enzyme can denature therefore destroy it. The enzyme that is in this experiment is Amylase. Amylase is found
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Genetic transformation of Escherichia coli with pGLO (Adapted from: Biotechnology Explorer: Bacterial Transformation: The pGLO System. Instructors Guide. BIO-RAD). Objectives a. To understand one of the most commonly used techniques for introducing DNA into E. coli cells and its use in molecular cloning. b. To become familiar with the concept of using green fluorescent protein (GFP) as a molecular tag for studying gene expression in bacteria and other organisms.
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Experiment #1: Carbohydrate Digestion • Tube 1 Digestion Lab – 3 ml water • Tube 2 – 3 ml 0.2% amylase • Tube 3 – 3 ml 0.2% amylase + 10 drops of 1.0M HCl • Tube 4 1 2 4 3 – 3 ml 0.2% amylase – place in hot water bath for 5 min Experiment #1: Carbohydrate Digestion • Add 5.0 ml starch solution to each tube • Incubate in 37°C bath for 1.5 hr • Divide contents of each tube evenly into 2 tubes – Lugol’s Test – Benedict’s Test Experiment #1: Carbohydrate
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Anaerobic Respiration Lab Aim: To identify the products of anaerobic respiration in yeast Apparatus and Materials: boiling tubes‚ delivery tube‚ bungs‚ sugar‚ yeast‚ lime water‚ liquid paraffin‚ Bunsen burner Procedure: Water was first boiled in the boiling tube. A small amount of sugar was then dissolved into the boiled water‚ which was allowed to cool. A little bit of yeast was added then stirred. Apparatus was set up as shown in Figure 4. A layer of liquid paraffin was added to the surface
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The purpose of this lab is to determine the rate of reaction under different circumstances. Different variables will be manipulated to discover their effects on a particular reaction. Changes in temperature‚ pH‚ and enzyme concentration are examples of factors that have the potential to affect the initial rate of an enzyme catalyzed reaction in a controlled experiment‚ whether it be speeding the reaction up or slowing it down. Part I of the experiment establishes a baseline that can be used to compare
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and Measure of Enzymes Activity Abstract This experiment investigates the effect that temperature has on the rate of activity of enzyme β-galactosidase and also the rate of β-galactosidase activity in different concentration of substrate over time. Ο-nitrophenylgalactoside (ONPG) is used as a substrate for β-galactosidase. A spectrophotometer is used to detect the change in colour of the substrate. Results show that increase in temperature up to 50oC speeds up the rate of enzyme activity and any
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The implication of t(1;11) chromosome translocation in Schizophrenia pathogenesis Schizophrenia is a severe mental illness that affects approximately 1% of the adult population.1 Due to its complexity‚ there are many contributions to the genesis of the disease including epigenetic‚ stochastic and environmental factors‚ yet studies on families and twins have proved that the genetic component plays a crucial role.2 Multiple Schizophrenia-associated loci have been identified‚ many of them‚ revealing
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