Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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glucose. Even if oxygen is there or not‚ enzymes are mediated in the cytoplasm. The electron transport chain‚ chemiosmosis‚ and aerobic respiration use NADH molecule (which it main purpose is to transport electrons form one molecule to another) for later purposes. The mitochondrial matrix receives pyruvate from the cytoplasm after it crosses over the mitochondrial membrane. When the pyruvate enters the Krebs cycle it goes through many stages of biochemical enzyme-catalyzed reactions. In more detail
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withstand abnormal changes in pH without denaturing itself. Salivary Amylase is an enzyme that acts upon any polysaccharide that enters the mouth (mainly starch). However‚ it has a few other clinical uses. It is used for the diagnosis of acute pancreatitis‚ an inflammation of the pancreas‚ and other medical conditions regarding Amylase levels in the body. Amylase’s first discovery in 1831 led to new experiments about this enzyme. By separating pancreatic Amylase from Trypsin‚ they were able to realize that
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The Effect of Concentration‚ pH and Temperature on Enzyme Activity Biology For Majors October 4‚ 2012 Abstract We examined the reaction an enzyme has when its concentration‚ pH and temperature are altered. In order to do this‚ we added different levels of pH into different test tubes with the enzyme (sucrose)‚ and substrate (sucrose)‚ and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature
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Introduction Chemical kinetics is the study of reaction rates. A reaction rate is the speed of the change in either reactants or products over a period of time. General kinetic rate equation is: Where [A] and [B] are the concentration of the species in the reaction. The variable k is the rate constant‚ which is a function of time and catalyst presence. The variables m and n are the order of reaction for their respective species concentration. The higher the value of the reaction order the
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In this lab we are going to be observing the decomposition of piglets over a month’s time. There are theory questions that have been given to us before and after the lab. We look back at our original theory to see where we went wrong‚ and then correct it. The lab was disgusting‚ surprising‚ and very interesting. The first questioned to be answered is which piglet decomposes faster‚ a piglet that is in its natural state‚ that is burnt‚ that is buried‚ and that is buried in a wooden box? With
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Instructions: ➢ Everything that is written in red needs to be erased or replaced by your text. ➢ Use the hand outs and photocopies I have given you as a guide‚ but do not copy everything. The IB Lab Report Title – Be certain it reflects clearly what is being done in the lab. 1. Introduction In this section‚ include the background/theoretical information on which your investigation is based. Explain the theory behind the experiment or maybe how this experiment models what you
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Lab Report #1 Title: Synthesis of Divanillin Abstract: The purpose of this experiment was to synthesize divanillin. This was done via the oxidative dimerization of two equivalents of vanillin‚ using enzyme horseradish peroxidase as the catalyst. Procedure: Lab Handout: Nishimura‚ R.T.; Giammanco‚ C.H.; Vosburg‚ D.A. J. Chem Educ. 2010‚ 87‚ 526-527. Discussion: Mechanism: Reaction 2 Vanillin H2O2 Divanillin 2 H2O
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Photosynthesis in Green Algae Background Photosynthesis is an amazing process where plants are able to create their own food as well as oxygen using sunlight‚ carbon dioxide (co2)‚ and water. The part of the plant responsible for photosynthesis is the enzymes in the chlorophyll (structures that carry out photosynthesis located in leaves). Photosynthesis is the process of creating glucose. Water + Carbon Dioxide + Sunlight = Glucose + Oxygen. There are three main factors that can influence the rate of photosynthesis--
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Investigation on Effects of Different pH on Enzyme Activity How does the different pH buffers affect activity of potato enzyme/extract? Introduction: Proteins are polymers that are made up of smaller units/monomers called amino acids. There are 20 different types of amino acids‚ thus make up many different combinations in types‚ numbers of amino acids as well as their orders – an explanantion for why there are so many proteins. Every protein‚ due to various reactions of amino acids to each
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