affecting Enzyme Activity: The effect of pH on enzyme activity Background Knowledge: An enzyme is a biological catalyst – which speeds up the reaction rate‚ without itself getting altered. Enzymes are proteins with long polypeptide chains that are folded up into three – dimensional shapes. An enzyme acts on a substrate to convert the substrate into a product useful for the organism.The active site is a special region on the surface of the enzyme where the substrate binds to the enzyme.
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The Effect of Temperature on Solubility By Aviraj Singh Rogers 2 Background: The solubility of most solid substances is generally said to increase as the temperature of the solvent increases. However‚ some substances‚ such as ytterbium sulfate‚ do the opposite. This can be explained through the Second Law of Thermodynamics which states that “in all energy exchanges‚ if no energy enters or leaves the system‚ the potential energy of the state will always be less than that of the initial state”
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WHY DOES THE COLOUR LEAK OUT OF COOKED BEETROOT? PLAN Hypothesis Temperature has an effect on the leaking of the colour from beetroot. This means that as the temperature is altered there will be a change in the rate of colour leakage. Scientific Background Beetroot is a very familiar vegetable and is commonly known as beet. It is famous in most recipe books that advice that its outer skin is not to be removed to avoid getting red dye in the cooking water. If we look at the internal structure
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Practical 2.1- The Effect of Temperature on Membranes Objective To investigate the effect of temperature on membrane structure Introduction Beetroot Pigments Beetroots contain Betalains which are the red pigments present in the cell vacuole. Betalains are soluble in water and they contain nitrogen. Betalains extracted from beetroot is commonly used as food dye because it is not known to cause any allergic reactions. Beetroot Picture taken from http://tipdeck/how-to-cook-beet-root Structure
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THE EFFECT OF TEMPERATURE ON CATALASE ACTIVITY AIM The aim of this experiment is to find out the effect of temperature on catalase or hydrogen peroxide. This will enable us to tell at what temperature hydrogen peroxide is most efficient. This (degradation) reaction will help us determine some of the catalase’s different attributes. HYPOTHESIS In this experiment it would be safe to hypothesise that no activity would take place at 1 to 20 degrees. It would be probable that a little activity would
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The decomposition of hydrogen peroxide (H2O2) in a whole and diced wedges by with The enzyme catalase was observed. The catalase was able to break down the hydrogen peroxide In the diced banana wedge better than the whole banana because after the banana was diced that Increases the surface area allowing the breakdown to flow. The effects of temperature on enzyme In a liver sample were observed under iced‚ boiling‚ 37 degrees‚ and room temperature Conditions. The enzymes became completely denatured
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Year 11 IB Biology IA Report 2011 5/31/2011 Enzyme Activity Experiment Introduction 3 AIM 3 Materials 3 Method 4 Result 5 Table 1: 5 Table 2: 5 Graph 1 6 Discussion 6 Conclusion 8 Bibliography 9 Appendix 1 (Test tube 3- 45 degrees) 9 Appendix 2 (Test tube 1- 5 degrees) 9 Appendix 3 (Test tube 2- 20 degrees) 9 Introduction Phenolphthalein is an indicator that is pink in alkaline solutions of about pH10. When the pH drops below pH 8.3 phenolphthalein will
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PROCEDURE: Part A (Effect of temperature on growth) 1) 15 tubes of glucose broth are provided and one set of 3 tubes are inoculated with each of the following cultures; Escherichia coli‚ Pseudomonas fluorescens‚ Micrococcus luteus and Saccharomyces cerevisiae. The last served as control. 2) One of the three tube of each culture is incubated at each of the following temperature: * 4°C * 37°C * 55°C 3) All the tubes are incubated within 5 minutes after inoculating. The turbidity
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Practical: The effect of pH on enzyme activity Rationale Amylase is an enzyme involved in the digestion system which catalyses the breakdown of starch into sugars. It is not only present in human saliva but also in the pancreas‚ where it hydrolyses dietary starch into disaccharides and trisaccharides which are converted by other enzymes to glucose to supply the body with energy. pH has an effect on the activity of all enzymes‚ including amylase. As the pH level increases‚ the enzyme activity increases
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The effect temperature has on the activity of the enzyme α- amylase. Introduction Enzymes are a biological catalysts‚ which means that they speed up the chemical reactions in living organisms. Almost all of enzymes are energized protein molecules that catalyse and regulate nearly all biochemical reactions that occur within the human body. The reason in which enzymes are sensitive to heat‚ pH and heavy metal ions is because they are made up of proteins. The food we eat is turned into energy by enzymes
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