Introduction: In liver cells‚ hydrogen peroxide is produced as a bi-product of some metabolic reactions. Hydrogen peroxide is a highly active chemical used in household products for bleaches and cleansing wounds. In a cell its build up would be highly toxic. However‚ liver cells contain an enzyme‚ catalase‚ which immediately breaks down hydrogen peroxide. It is a peroxidase and breaks up the toxic hydrogen peroxide to water and oxygen which are both nontoxic. The reaction is exothermic‚ meaning that
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THE EFFECT OF PH ON THE ACTIVITY OF CATALASE Aim : To study the effect of pH on the activity of catalase. Introduction : Catalase‚ an enzyme found in many different tissues‚ catalyses the breakdown of hydrogen peroxide into water and oxygen. 2 H202 → 2H20 + O2 Hydrogen peroxide is a toxic substance that can be formed during aerobic respiration and catalase removes this product. The activity of catalase can be measured by finding the rate of oxygen release from hydrogen peroxide. Potato
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20th‚ 2012 Enzyme Lab What is an enzyme? Enzymes are specialized protein molecules simplifying most of the body’s metabolic processes such as‚ supplying energy‚ digesting foods‚ purifying your blood‚ executing the body of waste products etc. Enzymes act as catalyst by speeding up the reactions that happen in our bodies and decreasing the amount of activation energy needed to break a complex down. A reactant is any given enzymatic reaction is called a substrate for that specific enzyme. The place
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Enzyme Activators and Inhibitors Lucia House AP Biology‚ Block 4 Mr. Trice October 18‚ 2012 Introduction: Metabolism is the totality of all of an organism’s chemical reactions. Chemical reactions occur due to enzymes‚ a substance which acts as a catalyst in driving chemical reactions in order to produce a desired product (Campbell and Reece‚ 2002). A catalyst is usually a protein; however‚ some catalytic molecules counter this generalization. A discovery made in the early nineteen- nineties
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Enzymes are catalysts that are used to speed up chemical reactions. Adding inorganic catalysts to reactants increase the rate of most chemical reactions. However‚ all enzymes are different and there are numerous amounts. Many enzymes are essential for life and reactions would not happen rapidly to maintain life with the help of enzymes. Specific enzymes lower the activation energy for specific reactions and shapes. Activation energy is required to start a chemical reaction. This occurs when energy
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Temperature effects on the growth of microorganisms Introduction: The purpose of this experiment was to determine the effects that temperature has on three different organisms. Temperature is one of the most important environmental factors affecting growth and survival of microorganisms². The three organisms used where Escherichia coli‚ Pseudomonas fluorescens‚ and Bacillus stearothermophilis. Most bacteria grow within a particular temperature range. The minimum growth temperature is
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Investigating the effect of temperature on plant cell membranes The purpose of this activity is: • to practise experimental and investigative skills • to investigate the effect of temperature on cell membrane structures Procedure SAFETY: Always carry scalpels clasped to a tile and with the tip pointing away from you. Beetroot cells contain pigments called betalains that give the tissue its dark purple-red colour. The pigment is contained in the cell vacuole. Investigation
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concentrations of hydrogen peroxide would affect the activity of catalase? 1. Variables Independent Concentration of hydrogen peroxide Dependent Volume of oxygen gas evolved Control Amount of liver used Location of liver sample obtained from the liver Type of liver used Possible enzymes transferred from hands during making liver homogenate Deterioration of liver Endpoint identification Volume of hydrogen peroxide added Reading on the syringe Room temperature 2. Hypothesis
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tube - Distilled water - Hydrogen peroxide - Knife Safety precautions: Dealing with corrosive chemicals “hydrogen peroxide” Method 1. Cut potatoes into 9 equal pieces. 2. Peel off skin. 3. Cut each potato chip into ascending order. 4. Add buffer of 4.8 ml. 5. Add 1.4 ml of hydrogen peroxide 6. Add potato chip & close bung. 7. Take measurement of gas after 2 minutes. 8. Repeat. Investigating the effect of enzyme concentration on the rate of an enzyme-catalysed reaction. James Moore
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Enzymes are a major role in the process of accelerating chemical reactions. Environmental factors affect the enzymes‚ These environmental factors include ph levels and body temperature. While both of these environmental factors affect the enzyme‚ body temperature affects the enzymes the most and can result in death. Enzymes are affected by high body temperature. Enzymes are highly specific and they work at certain temperature levels depending if it’s hot or cold. However‚ when the
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