1.7 Factors that affect the activity of an enzyme It is important when working with enzymes to understand basic enzymatic theory behind them when selecting conditions to measure the activity of the enzymes. The factors that are known to affect the concentration of enzymes are temperature‚ pH‚ concentration of enzyme‚ concentration of substrate‚ buffer type and concentration‚ the presence of any inhibitors and cofactors (Worthington-biochem.com). 1.7.1. Temperature With most catalysed reactions‚
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The Effect of Enzymes on Apple Juice Production Biology Period 6 Background Info The purpose of this experiment was to determine which enzyme or the combination of the two enzymes makes the most apple juice from applesauce. We did his by‚ mixing 10 drops of an enzyme (cellulose/pectinase/both) into a few tablespoons of applesauce. We then left the rest to nature‚ and watched the liquid funnel out for 10 minutes. Lastly‚ we recorded our data. The cell wall is a complicated
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October 29‚ 2012 The effect of temperature on beet cell membranes Introduction: Cell membrane is the plasma like substance that separates different organelles and molecules within an organism. The cell membranes vital function is to permit passage of protein and enzymes through their wall. Red beets‚ also known as Beta Vulgaris has a red pigment that is located in its large central vacuoles. It is my hypothesis that if the cell membrane is to experience extreme temperatures‚ then it’s most likely
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Computer Enzyme Action: Testing Catalase Activity 6A H2O2 is toxic to most living organisms. Many organisms are capable of enzymatically destroying the H2O2 before it can do much damage. H2O2 can be converted to oxygen and water‚ as follows: 2 H2O2 → 2 H2O + O2 Although this reaction occurs spontaneously‚ enzymes increase the rate considerably. At least two different enzymes are known to catalyze this reaction: catalase‚ found in animals and protists‚ and peroxidase‚ found in plants. A great
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The effect of temperature on the permeability of cell membranes Introduction For my coursework‚ I intend to assess how temperature affects the plasma membrane of a cell. For this I will use the cell of a beetroot. Background What is a cell membrane? The cell membrane‚ also called the plasma membrane is a semipermeable lipid bilayer which is the surface of all cells that surrounds the cytoplasm. The membrane is called a bilayer as it has a double layer of phospholipids. Within the cell membrane
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Effects of Different Concentration of Catalyse on Hydrogen Peroxide Aim: In this investigation I will try to find how long it takes for the filter paper disc to rise up whilst varying the amounts of concentration of catalyse. Prediction: I predict that the lower the concentration of catalyse the longer it will take for the filter paper disc to rise to the surface of the tube. Equipment: 1. Hydrogen peroxide in a container 2. Flat bottom tube 3. Tweezers 4. Filter paper
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Liver is one of the most important organs in human body‚ it helps in fighting infections‚ metabolizing drugs by producing enzymes‚ and synthesizing proteins like‚ albumin. Liver failure happens When the liver cannot adequately functions and it is life threatening condition if it’s not managed immediately‚ it might cause some complications‚ like cirrhosis‚ hepatitis and multi-organ damage.(1)Liver transplant might be a lifesaving solution with liver failure‚ unfortunately liver transplant have its
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Enzymes (pron.: /ˈɛnzaɪmz/) are large biological molecules responsible for the thousands of chemical interconversions that sustain life.[1][2] They are highly selective catalysts‚ greatly accelerating both the rate and specificity of metabolic reactions‚ from the digestion of food to the synthesis of DNA. Most enzymes are proteins‚ although some catalytic RNA molecules have been identified. Enzymes adopt a specific three-dimensional structure‚ and may employ organic (e.g. biotin) and inorganic (e
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Part one 1. a) When hydrogen peroxide was added to manganese‚ tiny bubbles formed in the solution- intensity of (1). b)The solution did not change in temperature. c) The bubbles stopped forming after about 18 minutes. 2. After we added 2 more mL of hydrogen peroxide‚ the reaction occurred again. Again‚ small bubbles formed; intensity of (1). The solution did not change in temperature. The reaction lasted about 15 minutes. 3. After adding more manganese dioxide‚ the reaction occurred again. This
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structures of cells but they act as enzymes in reactions of the body1. Enzymes are biological catalysts that lower the amount of activation energy needed in carrying out biochemical reactions1. Enzymes are responsible for almost every reaction that occurs in a cell and is named according to the substrate they specifically affect. An enzyme works best under optimal conditions pertaining to temperature‚ pH level and salt concentration1. In unfavorable conditions enzymes will become denatured and ineffective
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