How Environment Can Effect Enzymes Introduction: In a chemical reaction there sometimes can be a catalyst present known as an enzyme. An enzyme is a protein that speeds up a chemical reaction by lowering the amount of activation energy required to start the reaction. By lowering the activation energy‚ more of the substrate is able to participate in the reaction‚ speeding it up. Enzymes are substrate specific. The substrate is what the enzyme bonds to. That is to say that enzyme A will only react
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Chirag Patel Biology 157 Determining the Effect of Varying Substrate Concentration Question: How does concentration of potatoes affect the amount of oxygen produced with hydrogen peroxide and potatoes (enzymes)? Introduction The enzyme used for this experiment is Catalase. Catalase is inside mostly any living organism which uses oxygen. Its job is to break down hydrogen peroxide‚ into oxygen and water. (Formula) 2H2O2 ---> 2H2O + O2 (lab manual). There are limiting factors which if altered
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Jeffrey Shenfeld An Experimental and Statistical Analysis of SDH activity as compared in Liver‚ Kidney‚ and Heart Homogenates of the Bos taurus Methods The three tissues being analyzed in this experiment‚ those of the kidney‚ heart and liver‚ were taken from the animal Bos taurus. The tissue homogenates used were made by adding 1 gram of tissue to 20 ml of sucrose phosphate buffer. The buffer was composed of 250 mM sucrose‚ 50 mM NaPO4‚ with a pH of 7.4. This mixture was homogenized with
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Temperature and Thermometers The Temperature of an object is a measure of the hotness or coldness of that object. An alternative way to think of temperature is to say that “the temperature of an object is a number – on some manmade scale – that indicates the hotness of the object”. ‘Hotness’ in turn is a measure of the kinetic energy of the molecules of the material. Note: You must use the term ‘hotness’.* The SI unit of temperature is the Kelvin (K)* Relationship between degrees Celsius
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Investigation Into Temperature Sensors Introduction In this project I will be investigating how it is possible to use a temperature sensor to keep a greenhouse from changing temperature too much. This is intended to help plants live and grow in their optimum temperature. This will create a perfect temperature for the enzymes in the plants to work in‚ and therefore resulting in a maximum growth/production rate. This could be useful for gardeners who wish to grow plants as quickly as possible
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Experiment 4: Enzyme Kinetics. Results/Discussion Week 1 Part A: Table 1. Enzyme activity for each assay of 4-nitroaniline formation. Rate of 4-nitroaniline formation Name of trial Abs/sec Abs/min M/min mol/min µmol/min #1 0.00003 0.0018 2.05x10-7 2.15 x10-10 2.15 x10-4 # 2 0.00010 0.0060 6.81x10-7 7.15x10-10 7.15x10-4 # 3 0.00020 0.0120 1.36x10-6 1.43x10-9 1.43x10-3 # 4 0.00030 0.0180 2.00x10-6 2.10x10-9 2.10x10-3
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wire‚ the temperature of the wire will be increasing. Measure the temperature using the infrared thermometer. Record the ammeter reading as the temperature increases 10°C before disconnecting the wire from the circuit and measuring the wire resistance immediately using the ohm meter. 4. Repeat procedure 4 when the temperature of the wire increases every 10°C until the highest temperature is reached and the measurements are recorded. Conclusion The hypothesis that when the temperature of a wire
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Jolly Nguyen 11C Conditions needed for effective enzyme action Aim: To investigate the activity of enzymes and how might the activity be effected in different conditions. Hypothesis: Temperature rises and change of substrate concentration may cause denaturation of the protein of enzymes. So as the temperature rises the amount of active enzyme progressively decreases‚ and the rate is slowed. Exposure to heat causes atoms to vibrate violently and this disrupts bonds within globular proteins
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Abstract Enzymes are proteins that lower the activation energy needed for chemical reactions. The two main environmental factors that can affect the enzyme’s activity are temperature and pH‚ and each enzyme works best at a particular temperature and pH. The purpose of this enzyme kinetic experiment was to observe the effect of temperature and pH on the reaction of barley alpha-amylase enzyme with starch substrate and establish the optimum temperature and pH for this reaction. The optimum temperature
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MIC Practical Report Kerry Haarhoff 18 April 2012 3150540 Introduction Bacteria and fungi are both micro-organisms‚ however‚ fungi are spore-producing organisms whereas bacteria are not and fungi can be multicellular and bacteria is only a unicellular organism. These 2 micro-organisms‚ along with many other things circulate in the air within our environment. These micro-organisms then settle and become more prevalent in different areas. An experiment was conducted to see where
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