WESTERN BLOTTING (Written Report) I. PRINCIPLE Western blotting is a method for identifying a specific protein in a complex mixture and simultaneously determining its molecular weight. In Western blotting‚ proteins are electrophoresed into a gel‚ as the proteins migrate through the gel they are separated based upon size and charge. Characteristically‚ smaller proteins migrate through the gel faster than larger proteins. Sufficiently separated proteins in an SDS-PAGE can be transferred to a
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The Western Ghats or the Sahyādri constitute a mountain range along the western side of India. It is a UNESCO World Heritage Site and is one of the eight hottest hotspots of biological diversity in the world. It is sometimes called the Great Escarpment of India. The range runs north to south along the western edge of the Deccan Plateau‚ and separates the plateau from a narrow coastal plain along the Arabian Sea. The range starts near the border of Gujarat and Maharashtra‚ south of the Tapti river
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there a heaven and hell? Eastern and Western philosophy are similar but different in many ways. Eastern philosophy includes the various philosophies of ancient China and India‚ but can also include Islamic‚ Jewish‚ and Persian philosophies. Western philosophy refers to the philosophies developed by the ancient Greeks and Indians. There are many differences between Eastern and Western philosophy. One is the east uses concepts by intuition while the west uses
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Name: Tutor/ professor: Institution: The western tradition Introduction Renaissance is a French term meaning rebirth is a perfect description of the economic as well as intellectual changes that happened in Europe continent from fourteenth century through the sixteenth century. In the course of the renaissance era‚ the whole of Europe sprung from stagnation in terms of economic status which was dominant in the middle ages to a period of economic or financial prominence. Of much greater importance
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Western Blotting is used to Identify a Subunit of Myosin Light Chain in the Proteins of All the Different Fish. Chae Yee Kent State University: BSCI 30140 Lab Abstract Western Blotting can be used to detect the Myosin actin light chain in different species of fish and is used to distinguish from different species based on variation‚ commonality‚ or evolutionary divergence. First‚ proteins are extracted from the tissue and loaded into a gel matrix. The matrix will separate the proteins according
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In Handmaid’s tale (novel) there are couple symbols such as Handmaid’s red habit‚ flowers‚ scribble‚ and Harvard’s Wall. As we can notice in Novel‚ Offred often uses the symbols such as different colors. For example‚ the red color of costumes which were worn by Handmaid’s symbolizes fruitfulness‚ which they bear a child and it’s also a uniform color for the Handmaid’s. According to Offred” red: the color of blood‚ which defines us” (p. 8‚9). Red also symbolized the menstrual cycle and childbirth
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Letter The Scarlet Letter is a novel filled with important symbols‚ each with many meanings. Nathaniel Hawthorne‚ author of The Scarlet Letter‚ knew how to express these symbolswith a creative tough. He used the “A”‚ the scaffold‚ and the prison to describe the Puritan society. The symbols or The Scarlet Letter help create a theme‚ the conflict‚ and the characters. The scarlet “A” makes its way to be the most important symbol in the novel. The “A” represents adultery‚ which is a violation
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Pick Two Symbols from The Great Gatsby and Explain Their Relevance/Significance In The Great Gatsby‚ by F. Scott Fitzgerald‚ the use of symbols throughout the book is very evident‚ and plays a large role in the book to help convey different underlying meanings. Two of the most apparent symbols in The Great Gatsby are colors‚ including the green light‚ and Dr. T.J. Eckleburg’s eyes on the billboard in the Valley of the Ashes. Colors symbolize a great deal in The Great Gatsby‚ and different colors
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antiquity that exists in the Eastern cultures. We think 300 years is a long time when in Europe traditions can be traced back a couple thousand years and in India they can trace Hinduism and their way of life back 4‚000 years. The very nature of western religion is nothing but change. It has its roots in Greek and Roman polytheism and to go from polytheistic to monotheistic is a huge‚ 180 degree turn and about the biggest change
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A symbol is a specific word‚ idea or object that may stand for ideas‚ values‚ persons or ways of life. The personal narratives “The Scholarship Jacket" by Marta Salinas‚ "The Jacket" by Gary Soto and "Vinnie ’s Jacket" by Anna Nussbaum all have a jacket as the central symbol. In each of these works‚ however‚ the jacket represents very different things. For Marta Salinas in "The Scholarship Jacket"‚ the jacket symbolizes eight years of hard work and expectation. Throughout the narrator ’s life
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