Lab 11 Methodology By using aseptic‚ a little cultured bacteria was inoculated on the TSA agar. A quadric streak was making. Inoculation loop was heated and keep it cold for a while before the next quadratic streak. Six agar plates were observed for 24 hour at temperature of 30ºC. Choose one from the dense colony and make a sub-culture on the new agar plate. The step was repeated to get a single colony‚ which is pure colony. a) Sequestration of bacteria from fish organs Methodology
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Agar and Media Preparation— Agar plates containing King’s B Agar were often used throughout the experiment to support growth of Pseduomonas fluorescens. A recipe was used that included a mixture of 10g Proteose Peptone #3‚ 1.5g Potassium Phosphate Dibasic (K2HPO4)‚ 30ml 50% Glycerol‚ ~965ml water and 20g agar. The mixture‚ post- autoclave‚ was left to cool and 5ml 1M Magnesium Sulfate (MgSO4) was added and created about 40 plates. King’s B Medium was made using the same procedure as the King’s B
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LAB REPORT WOODLICE Daan Rijpkema May 2009 T4Y - General Science X Words TABLE OF CONTENTS Aim..........................................................................................................................................................2 Hypothesis...............................................................................................................................................2 Materials ............................................................................
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prediction made was the planarian that was the longest‚ would regenerate the quickest and begin to behave normally first. Listed materials Seven clear petri dishes Colored utensil to label Purified water Boiled eggs Tooth picks Flashlight Measuring tape Paintbrush Knife Procedure 1. Obtain six petri dishes 2. Put enough water into the petri dishes to cover the bottom of all six 3. Label the three dishes with the colored utensil with three names of choice (We choose Chubbs‚
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concentrations of three chemicals‚ ammonia will have the highest LC-50 (toxicity)‚ bleach will have the intermediate LC-50 (toxicity) and vinegar will have the lowest LC-50 (toxicity). MATERIALS AND METHODS: For this experiment we use the following materials: Petri dishes (4 per group) Stop watch or clock Safety goggles (1 for each person) Brine shrimp Brine solution (3.5% w/v NaCl and water) need 600 ml per class Vinegar- need about 50 ml in small dropper bottle (1 per group) Ammonia- need about 50 ml in small
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For this experiment petri-dish A is fully exposed to the light source while petri-dish B is partially exposed to the light. Independent Variable: We have chosen our independent variable to be the amount of light exposed to the cress seeds. Our first petri-dish was allowed to be fully exposed to the light surrounding it‚ while the second was fully wrapped in black paper excluding a 0.5cm slit directed towards the sun. Dependent Variable: Our dependent variable is the reaction/actions to
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noticed that there was a small circle around the tree that had nothing growing on it and we noticed that there were so many leaves that any living thing under there would be soaking in eucalyptus tea after it rained. We set up the experiment by having petri dishes and labeling them on the bottom so they won’t get mixed up. It was very easy to set up. We just had to make the tea from the leaves we got from the eucalyptus trees‚ since it was a known allelopathic plant. We had 2 of the same things grow in
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Singapore 288683 ABSTRACT In this project‚ the relationship between the concentration of salt solution and the length of the potato strip that was immersed in it was investigated. Five strips of potato of the same length were placed in different Petri dishes that contained water of different salt concentration. They were left for twenty minutes and the length of each potato strip was recorded after the experiment. The experimental results show that the length of the potato strip increased when placed
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around bacteria. I was suggested to do a project where I get a petri dish containing my oral bacteria and see the effects of liquid on my oral bacteria. I was very curious about this topic and wanted to know how the bacteria grows so I gave it a shot. For my project‚ I was told to make an “if and then” hypothesis. My hypothesis was that “If I put orange juice on a petri dish containing my oral bacteria then the bacteria on the petri dish will multiply.” For this experiment‚ I gathered all the materials
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Clare Worley 6S 4 - 16 - 18 What Surface in OLV has the most Germs? Introduction I have tested several surfaces in Our Lady of Victory to see which surface has the most germs. I tested ten surfaces and let the petri dishes grow for five days. Over the five days I counted the spores each day and took pictures every other day. It is amazing how things can look and feel clean but they are actually filthy. It is also crazy how many surfaces we touch every day and how one
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