between the amount of substrate in the assay solution and the rate of the reaction when the enzyme and buffer in the assay are held constant were experimented. We analyzed the change in absorbencies over time for varying substrate concentrations. There were four experimental assays which contained 1% enzyme solution‚ substrate solution of 0%‚ 1%‚ 2%‚ and 3% concentrations‚ guaiacol‚ and pH 7 buffer. At 2% concentration there was a greater enzymatic activity and at 3% concentration enzymatic activity
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find the pKa of an unknown‚ pH meters are used during titrations to measure the potential difference in a solution by measure the difference of hydrogen-ion activity in a solution and reporting the pH. The pH is then plotted against the volume of solution added which provides the titration curve of a substance. From the titration curve information can be gathered about how many protons were dissociated and the equivalence point. pH=pKa+log([H]/[OH] ) pKa=pH-log([H]/[OH] )
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:___________ Chemistry Laboratory 101__ Date Submitted[1] :___________ Members[2]: Instructor’s Initials[3] :___________ 1. _____________________ 2. _____________________ 3. _____________________ 4. _____________________ Laboratory Report Sheet The Bunsen Burner Activity 1 Objectives:4 1. ________________________________________________________ 2. ________________________________________________________ 3. ________________________________________________________
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Introduction! ! The Michelson Interferometer is commonly used to determine the wavelength of light or measure very small distances. It was invented by Albert Abraham Michelson and is commonly used in optical interferometry‚ a branch of physics involving a family of techniques one could use to extract information about waves by superimposing them. ! ! The original application of the Michelson Interferometer was to the famous Michelson-Morley experiment in 1887. Prior to Einstein’s
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Determining the habitat preference of Artemia franciscana by creating artificial gradients of three abiotic factors: light‚ pH‚ and temperature Introduction In the study of ecology‚ species are often described as generalists if they live in broad environmental conditions or specialists if they live in narrow or specific environmental conditions (Reece et al. 2011). Artemia franciscana‚ the organism under study‚ is a well known generalist that has adapted it’s reproductive strategy to allow
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hypnotized that the enzyme is acidic on the pH scale because it exists in our small intestine and our small intestine uses acid to break down food. The result from the lab prove my hypothesis correct because lactase worked well at body temperature and it also worked well at pH of 4.5 to 5.5 mg/dl proving that lactase works best at an acidic pH number. The lab shows that the highest amount of glucose is made by temperatures close to body temperature. In our our lab‚ we found that at 35 °C 500 mg/dl glucose
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TITLE OF EXPERIMENT 2 : DILUTION 2.1: OBJECTIVES Upon completion of this experiment‚ students should be able to: 1. determine the concentration of coloured solution such as FeCl3 by using dilution and colour differentiating (colorimetric) techniques. 2.2: INTRODUCTION Concentration can be expressed in many different ways such as percentage volume and percentage weight etc. In laboratory‚ normally concentrations were expressed as molarity and normality. Molarity is the
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December 8‚ 2013 Soil Analysis Lab Report Intro: Soil is produced over hundreds of years through the weathering of rocks and the decomposition of organic material. Soil contains nutrients that are taken from decomposed animals and plants which turn to detritus. Nutrients such as nitrogen are added to the soil in these ways by organisms such as nitrogen fixing bacteria. Soil is important because it is the fuel to the plants that we need in order to survive and for all species to survive. Soil
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OLD COINS TURN TO GOLD DESIGN: Problem or Research Question: How does zinc effects the color change in a copper penny? Hypothesis: If copper and zinc comes together‚ then it will form brass‚ which gives gold color to copper penny. Variables: There were no variables at this experiment PROCEDURES: Materials: Zinc (SN) filling‚ 3M NaOH solution‚ Copper penny‚ tongs‚ Hot plate‚ 100 ml beaker‚ 250 ml beaker‚ Bunsen burner‚ Water‚ Spoon. Procedure: First‚ we turned on the hot plate.
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Enzyme Lab Report Introduction The objective of this experiment was to determine if changes in pH or temperature affected the activity of enzymes‚ specifically the enzyme sucrase. Enzymes are protein molecules that act as biological catalysts to increase the speed of the reaction or to lower the activation energy of that reaction. However‚ the activity of an enzyme can be affected by physical factors such as pH and temperature because these factors alter the structure of the enzyme (Freeman‚ 2011)
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