Title : Measuremnt of pH With Indicators Aim : 1.To investigate concentration of hydrogen ions in hydrochloric acic and colour changes of indicators 2.To determine standard solutions and the unknowns Variables Independent variable : Concentration of hydrogen ions in hydrochloric acid.The presence of hydrogen ions is varied by using 5 different concentration of hydrochloric acid of 0.1mol/L‚0.01mol/L‚0.001mol/L‚0.0001mol/L‚0.0000mol/L Dependent variable : Colour changes of indicators
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Effects of Temperature and pH on Enzyme Function Chelsie Mesa Section 0479 Robin Cotter Introduction The purpose of this experiment is to identify three unknown enzymes. This is done by using different temperature and pH to affect the function of the enzyme‚ which ultimately‚ will affect how much maltose is produced. Enzymes are proteins that help catalyze chemical reactions. In enzymatic reactions‚ the molecules at the beginning of the process are called substrates‚ and the enzyme
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Abstract Photosynthesis is a food making process for algae and plants. The photosynthesis process rate varies from different wavelengths and intensities of light. This lab will evaluate the optimal wavelengths and degrees of intensity during photosynthesis when chloroplast is exposed to light. The mixtures of DCPIP with water‚ PO4 buffer‚ and chloroplast will be prepared in a number of cuvettes. The cuvettes were tested individually at different wavelengths and intensities to find the optimal rate of
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Results: For the solutions acid and bases lab the results my group received are as followed. Procedure 5.1 were we had to measure the ph of the following substances Vinegar 4 Apple Juice 4 Black coffee 5 Baking Soda + Sprite 8 0.01mM HCl 4 0.1mM HCl 3 Distilled water 4.5 Tap Water 5 Procedure 5.2 -Test the ability of buffers Before Buffer After Buffer Water 4 Water 4 0.1M phosphate buffer 6.5 0.1M Phosphate buffer 6.9 0.1M NaCl 4 0.1M NaCl 4.8 Procedure 5
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Conditions of Temperature‚ pH‚ Boiling and Competitive Inhibitors By Robin Caserta BIO 101 September 30‚ 2013 ABSTRACT The enzyme‚ peroxidase‚ extracted from a turnip was tested for its efficiency in binding to its substrate and its stability under several conditions. To do this‚ we tested effects on peroxidase activity‚ first‚ with different amounts of the enzyme‚ next at temperatures of 4oC‚ Room Temperature‚ 32oC‚ 48oC and boiling; then‚ at pH 3‚ pH 5‚ pH 7 and pH 9; and‚ finally‚ with the
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EXPERIMENT NO. 1 INTRODUCTION TO LAB INSTRUMENTS. 1. BREADBOARD We should be familiar to the following things about a breadboard: * What is a breadboard and what is it used for? * How does it work? * Setting Up. * Limitations. What is a breadboard and what is it used for? A breadboard (or protoboard) is usually a construction base for prototyping of electronics. The term "breadboard" is commonly used to refer to a solderless breadboard (plugboard). It was designed by
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Title: Enzyme Catalysis of Hydrogen Peroxide by Catalase Problem and Objectives: How do different temperatures and different levels of pH affect the reaction rate of the enzymes in chicken liver? Demonstrate the activity of an enzyme in living tissue‚ observe the effects of changes in temperature and pH on the activity of an enzyme‚ perform analyses for the presence of an enzyme in tissues‚ and analyzing relationships between environmental conditions and enzyme activity. Background: Cells produce
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The purpose of this lab was to determine the effects of changes in variables such as temperature‚ pH and concentration on catalase enzyme activity. Proteins are organic compounds made up of amino acid subunits consisting of carboxyl and amino functional groups‚ linked together by peptide bonds through condensation reactions. They are an essential part of all living organisms. Enzymes are “biological catalyst used to speed up chemical reactions (Athanasopoulos‚ 2014). Normally‚ for chemical reactions;
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AP Biology Lab: Catalase (Enzymes) Abstract In this laboratory exercise‚ studies of enzyme catalase‚ which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme
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diluted using TEA buffer to obtain a concentration of 10ng/µl) 2.50 Taq 2x Master Mix 12.50 Amelogenin Forward Primer(10µM) [primer sequence: GCTCTGATGGTTGGCC]
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