Bases‚ Buffers and Respiration Darlene E. McDonald WCCC Fall 2012 Ap Lab 071-07 Dr. Roxanne Levandosky August 31‚ 2012 I. INTRODUCTION: A.PURPOSE: a. To define and understand the key terms. ~acid ~buffer system ~base ~acidosis ~pH ~alkalosis ~pH scale ~hypoventilation ~pH indicators ~hyperventilation b. To determine the acidic or base nature and actual pH of various substances. c. To discuss the formation of the carbonic acid/bicarbonate ion buffer system
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in abnormal quantities present in the urine. APPARATUS : 1) Test tubes 2) Boiling water bath 3) Microscope 4) Slides and cover slips 5) Pasteur pipettes MATERIALS: 1) pH test strips 2) Benedict’s reagent PROCEDURES: Collect the urine samples in a container. Make sure to not spill anywhere in the lab. Complete the tests and all results/ observation are in a proper table. (A) Observation of urine 1) The urine is sniffed and the description of the urine is described. Terminologies
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Protocol for Lab 5 – Aerobic Respiration Part 1 Isolation of Mitochondria from Cauliflower - Weigh 50g of rosettes cut from fresh cauliflower head. - Cut rosettes and place it on ice - Prepare juice extractor by placing ice and an empty 150 ml beaker into the right compartment. - Collect pulp from left compartment and record total volume of the extract. Approx. 20ml - Filter the pulp using six layered cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes
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Introduction to Microbiology Laboratory report №10 Physical factors affecting growth microbes: Temperature‚ pH and oxygen requirement. Student: Temirlan Aitbekov Lab partner: Kanat Sadykov Instructor: Alessandra Clementi‚ MD‚GP Lab date: 7/11/14 Due date: 14/11/14 Nazarbayev University Abstract: This experiment is directed
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Proteomics is the study of proteomes‚ which are groups of proteins in living systems. It is used to examine when and where proteins are being expressed‚ rate of degradation‚ modifications‚ how they interact with other proteins and more. The goal in this lab was to determine the similarities and differences in muscle proteins among 5 species of fish using SDS-PAGE and Western blotting. First‚ proteins are separated based on size. Second‚ antibodies are used to detect the protein of interest. Lastly‚ a substrate
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the SDS-PAGE and Simple-PAGE gels were prepared with 3.25mL water‚ 2.0mL 4X resolving buffer‚ 2.7mL 40% acrylamide solution‚ 80μL 10% APS‚ and 3μL TEMED. The stacking layers for the SDS-PAGE and Simple-PAGE gels were prepared with 4.6mL water‚ 2.0mL 4X stacking buffer‚ 1.3mL 40% acrylamide solution‚ 48μL 10% APS‚ and 5μL TEMED. The SDS-PAGE gel used the buffers containing SDS and the Simple-PAGE gels used the buffers without SDS. The gels were run at 100 V for about 60
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us determine our data. The purpose of this experiment is to follow the changes of pH during the titration of an acid and a base in order to determine the of the weak acid‚ . is a constant for a given acid at a given temperature. In this experiment we determined the Ka using two different methods: 1) the measurement of the pH of a solution containing a known concentration of a weak acid‚ and 2) measurement of the pH at the half-neutralization point in the titration of a weak acid and a strong base
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Lab Report: Understanding Photosynthesis Gen Biology Lab Abstract: This lab was called photosynthesis: understanding photosynthesis. It is a highly complex process that needs to be broken down in many steps to understand how it works. This lab covers the big components in photosynthesis including carbon dioxide intake‚ light consumption‚ and varying pigmentation. Introduction: Photosynthesis is a huge concept to learn and understand in the field of biology. Plants have their own special
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Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
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City University of Hong Kong Dept. of Physics & Materials Science AP2104 Mechanics of Solids Laboratory Manual Experiment 1 Pure Bending of a Beam Experiment 2 Torsional Deformations Experiment 3 Yield Criteria for Ductile Materials under Plane Stresses Experiment 1 Pure Bending of a Beam Objective 1. To examine the stresses at various positions of the beam under a constant load of pure bending. 2. To determine the curvature of deflection of the beam. Introduction
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