attributes of yoghurt. In this experiment‚ dadih was prepared by acid method and enzymatic method. Eight formulations were used. The first 4 formulations by using acid method while the other four formulations by adding different thickening agent. The pH of dadih produced using the eighth formulation is 6.05 while the o Brix obtained is 17.0. On the other hand‚ the viscosity is 0.6594. For sensory evaluation‚ the scores obtained for texture‚ flavour‚
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Arielle Fisher GFP Lab November 16‚ 2011 Figure 1 A. B. C. D. Figure 1. Confocal images at 400x magnification of HeLa cells to locate GFP activity. HeLa cells were (A) tagged with Green Fluorescent Protein (GFP) (B) labeled with GFP and 14.13 µl Dexamethasone (C) tagged with GFP fused to the glucocorticoid binding
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The purpose of this experiment was to determine the weight percentage of copper in brass. This was achieved by performing a redox titration known as iodometry. Copper‚ which was obtained from the brass‚ was used to react with iodide to produce iodine. Iodine was then titrated with thiosulfate. In this experiment‚ the amount of titrant dispensed correlates with the amount of copper; therefore‚ the amount of copper in brass was calculated by using the data recorded. In this experiment‚ the analyte
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Post lab The unknown solution 4055 was tested by the separation scheme in order to identify the four cations that exist in it. The cations found were Ag+‚ Ba2+‚ Mn2+‚ and Ni2+. When I added 5 drops of 3M NaCl to the unknown solution‚ we had some precipitate‚ we centrifuge and then we divided to two test tubes‚ and they were put in hot water and got confirmatory tested. The Ag+ appeared to be in the solution because it melted‚ however Pb2+ didn’t. With the rest of the solution we added Na2SO4 we had
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[13]. LD50 of fenofibrate (standard) was found to be >1242 mg/kg‚ p.o. the effective dose of fenofibrate was considered to be 65mg/kg‚ b.w‚ orally. The solubility of serratiopeptidase was found to be in 2:3 solutions of DMF and PBS (phosphate buffer solution) of pH-7.2. Single dose of Triton X 100 (100mg/kg‚ b.w.) [13] was administered intraperitoneally to the groups II‚ III‚ IV‚ V. Normal controls were injected with saline alone. Triton X-100 was dissolved in freshly prepared‚ 0.9% sodium chloride immediately
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objective of this lab is to determine the pH level of common household products. Introduction Liquids are either acidic‚ basic‚ or neutral. We are able to figure out which one it is based on properties‚ along with the pH. Acids taste sour‚ and bases taste bitter. Using the pH scale‚ you are able to know how strong of an acid or base a liquid is. High concentration of [H+] ions means that the liquid has a low pH. Therefore a liquid with a low concentration of [H+] would have a high pH. A pH of less than
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Laboratory Report –Soils Soil ph Soil texture Soil organic matter Soil water retention Two soil samples were consistently used for all four test completed below. The first is labelled as Valley A‚ and was obtained from a field which has been known to be uncultivated for 10 years in the valley. Valley B was obtained from a domestic garden‚ under a Magnolia tree in the same region. At times when a third sample was tested‚ the source will be indicated
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phosphate buffer pH6 containing the enzyme: polyphenol-oxidase‚ was used as the variable being tested in this experiment. You may refer to the Enzymatic Reactions Biology 21 Lab Manual. Santa Monica College by Logan‚ R. 2003 to see how the potatoes extract in phosphate buffer was made. We began this experiment by measuring seven constant amounts of 1ml of 0.1% catechol using a 1 mL pipet into each seven cuvettes. The catechol is our substrate solution. Next‚ different amounts of phosphate buffer ph.6 of
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shows RFU’s (left) and total protein in ug (right) for each wash and elution. Once the rGFP had been purified in the Ni2+-agarose column‚ a breaking buffer (10mM Tris‚ pH 8.0; 150mM NaCl) was used to create the washes by pipetting the buffer in 0.5ml increments and collecting each in separate tubes labeling them W1-W6. Then‚ an elution buffer (10mM Tris‚ pH 8.0; 150mM NaCl; 300mM imidazole) was collected in increments of 0.5ml and labeled E1-E6. We could measure the amount of RFU’s each sample had using
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Biology 160 Lab Manual Tacoma Community College Last update 06/03/2013 This page left intentionally blank Table of Contents Table of Contents i Laboratory 1: Principles of the Scientific Method 4 In-Lab Report Sheet 12 This page has been left intentionally blank 27 Laboratory 2: Scientific Methodology & Enzyme Activity 28 In-Lab Report Sheet 28 Objectives 33 Introduction 33 Parts of the Swift M5 Microscope 33
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