Conclusion Both Kool Aid and Gatorade contain buffers. For the buffer in Kool Aid‚ pKa1 ≈ 3.70‚ pKa2 ≈ 4.90‚ pKa3 ≈ 6.50 and for the buffer in Gatorade‚ pKa1 ≈ 4.50‚ pKa2 ≈ 5.40‚ pKa3 ≈ 6.70. Discussion of Theory A buffer capacity is the maximum amount of hyrdrogen or hydroxide ion that can be added to a buffered solution before a significant change in the pH occurs. Increasing the concentration of the buffer can increase the buffer capacity of a solution. At the half equivalence point‚ the moles
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Enzyme Activators and Inhibitors Lucia House AP Biology‚ Block 4 Mr. Trice October 18‚ 2012 Introduction: Metabolism is the totality of all of an organism’s chemical reactions. Chemical reactions occur due to enzymes‚ a substance which acts as a catalyst in driving chemical reactions in order to produce a desired product (Campbell and Reece‚ 2002). A catalyst is usually a protein; however‚ some catalytic molecules counter this generalization. A discovery made in the early nineteen- nineties
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Abstract This lab was performed in order to discover the activity of the enzyme catecholase in different pH levels as well as its absorbance in differently concentrated solutions. A spetrophotometer was used to measure the absorbance of the enzyme catecholase in different pH solutions as well as to measure the absorbance of catecholase in solutions with different concentrations of potato juice and phosphate buffers. Absorbance of the enzyme catecholase was at an optimum level when pH was close to
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EFFECT OF pH LEVEL ON THE ENZYME ACTIVITY OF INVERTASE John Dale Cruz‚ Michael Ray Dela Cruz‚ Karen Angelica Domalaon‚ Abegail Dulay‚ Shirley Ann Felipe Group 5 2C Medical Technology Biochemistry Laboratory ABSTRACT Invertase is a type of enzyme‚ a natural catalytic agent for biochemical reactions‚ can be obtained in Baker’s Yeast. Determination of the effect of pH on invertase activity is the primary objective of the experiment. Dinitrosalicyclic acid (DNS) Assay method is utilized to monitor
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The Affects of pH on Peroxidase Enzyme Claire Bell Biology 1130 Jazmen Myers Section 36 Abstract Peroxidase is a turnip enzyme; it is used in the oxidation of hydrogen peroxide to lower activation energy‚ speeding up the reaction. The activity of peroxidase is highly dependent on its environment and most importantly the pH level. Peroxidase has been the focus of many recent studies and is believed to possibly reduce swelling among other things. We conducted an experiment testing
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Red Cabbage Indicator Aim – To create a pH indicator out of a red cabbage solution and to construct a basic pH scale to determine the pH of unknown solutions. Materials - • red cabbage leaves • 250 mL beaker • hotplate or Bunsen burner‚ tripod‚ gauze mat and bench mat • 10 test-tubes – equal size • test-tube rack Methods - Part A: Making the indicator 1. Tear up one or two red cabbage leaves‚ and place them in the beaker with enough water so that the cabbage is just covered. 2. Heat the beaker
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the effects of an incorrect pH level. pH measures the concentration of hydronium ions and can be modeled by the function p(t) = −log10t. The variable t represents the amount of hydronium ions; p(t) gives the resulting pH level. Water at 25 degrees Celsius has a pH of 7. Anything that has a pH less than 7 is called acidic‚ a pH above 7 is basic‚ or alkaline. Seawater has a pH just more than 8‚ whereas lemonade has a pH of approximately 3. 1) Create a graph of the pH function either by hand or using
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digestive power of pepsin is greatest at the acidity of normal gastric juice (pH 1.5–2.5). In the intestine the gastric acids are neutralized (pH 7)‚ and pepsin is no longer effective. "Pepsin (biochemistry)." Encyclopedia Britannica Online. Encyclopedia Britannica‚ n.d. Web. 10 Dec. 2012. Research Question: Does changing the pH of pepsin affect the ability to break down the protein (mass of jello). Variables: independent- Ph level dependent- The mass of the jello
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University’s wooded areas‚ the oldest was created in 1989 the second was created in 1994 and the youngest plot was created in 1999. For the hypothesis to be tested‚ there were soil PH meters to test the amount of PH in each plot’s soil. The hypothesis was incorrect; there was not a significant difference in the means of the PH from the oldest plot compared to the youngest plot. Introduction The definition of vegetation is plants considered collectively especially those found in a particular area
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Using Buffers Gino A. Romeo‚ Jr.‚ Ph.D. Version 42-0134-00-01 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information
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