in neutral pH. To do this the experiment was carried out so that tubes containing a reaction solution of the Amylase enzyme and starch were simultaneously mixed. The reactions were then introduced to I2-KI‚ which stopped the reactions‚ at two minute intervals. Each of these trials was repeated three times to ensure proper accuracy. After concluding the reactions they were placed into a spectrophotometer (A580) for analysis. Graphing the values of the absorbance to time for each pH it was found
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Research Question: To investigate how varying the pH of bromothymol blue affects the absorbance value of the solution which determines the equilibrium constant (pKa) of the indicator. Variables: Variables Variables Measured Method of measurement Independent pH of the six buffer solution A pH probe attached to a data-logger will be used to measure pH Dependent Absorption of the buffer solutions at wavelength 435.0nm and 617.0 nm A spectrophotometer (±0.001) will be used to measure absorbance
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Lab Report: Effects of Lysozyme Introduction: This report discusses an experiment that was done to demonstrate the effects of lysozyme on populations of Gram positive and Gram negative bacteria. Bacteria have a cell wall composed of peptidoglycan that gives the wall its strength. Gram negative bacteria have and extra component of lipopolysaccharide (LPS)‚ that is stabilized with magnesium ions‚ to their cell wall that further protects them. When Gram positive bacteria are treated with lysozyme
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During the preparation of the 0.100M Tris buffer‚ the calculated amount of ingredients brought the solution to a pH of 7.0‚ but the desired pH was 7.50. Discrepancies between the theoretically calculated amounts and the actual measured amounts of ingredients are likely to be the biggest source of error. Dilution affected our 0.0100M Tris buffer by decreasing its pH. The buffer was originally set to a pH of 7.48‚ but the pH gradually moved down by a pH unit of about 0.1 after each dilution. This is
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Titration Lab Title Abstract During this experiment the change in pH of Citric acids is measured from start to equilibrium in mL. This experiment was tested by titration it had two separate trials. Through observations it was shown that the more concentrated acids needed more drops of NaOH to reach its equilibrium then the less concentrated acids. Introduction Sodium Hydroxide (NaOH) is a strong base and when it is titrated with a strong acid the equivalence point will be expected to be a pH of 7
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Objectives: Review Guide for PH 150E Red= Source of Info Blue= Objective Black= answer Side note from review... Diff between social networks and social support: social networks--> social interactions social support--> derived resources from social networks. (ex of resources: be able to borrow money in case of emergency‚ emergency child care... basically someone to rely on in case of need) Politics of placemaking: zoning‚ redlining‚ why does development occur in some places vs others
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Research Question How will the addition of different pH buffers to amylase affect the rate of starch digestion measured using starch and iodine? Introduction Amylase is an enzyme found in human saliva and pancreas. It is the digestive enzyme that is needed to breakdown starch molecules. Amylase must be kept at certain conditions to function at its optimum level. This experiment will explore the effect of pH (1‚ 4‚ 7‚ 10‚ and 14) on the function of amylase by using starch and iodine. Usually
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Purpose: The purpose of this lab was to test how objects slightly acidic or slightly basic will affect the way catalase‚ the enzyme tested‚ denatures‚ or breaks down. Hypothesis: If the potato is acidic‚ it will react with the H2O2 more than it will with the raw‚ plain potato because the acid will denature the enzyme faster. The manipulated/independent variable is the raw‚ plain potato while the responding/dependent variable is the other types of potatoes used in the experiment. Materials:
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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