Chemistry 3373F Lab Manual 2008 Modified 11/07 Table of Contents Chem 3373 Laboratory Schedule for Fall 2008.............................................................................2 The Benzoin Condensation of Benzaldehyde ..............................................................................3 Synthesis of Dilantin and Related Compounds (two weeks).........................................................6 Synthesis of an Alkaloid: Pseudopelletierine (two weeks) .............
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Littlefield Labs 1 Capacity Management at Littlefield Labs I. Introduction There are 3 stations in the game called sample preparing‚ testing‚ and centrifuging‚ while there are 4 steps to process the jobs. Before the game started‚ we tried to familiarize with the process of the laboratories and calculating the costs (both fixed and variable costs) based on the information on the sheet given. We did not intend to buy any machines too early‚ as we wanted to see the demand fluctuation and the
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Introduction: The purpose of this lab was to measure the extent of enzyme reaction on given substrates by means of color change. The reaction followed is given below: Tyrosinase³ Enzyme Pyrocatechol Hydroxyquinone Oxidation/Reduction Pink ³ Brown E+S + [ES] = E+P Enzyme Reaction Hypothesis: If there is an increase in enzyme concentration‚ an increase in reaction temperature‚ or an increase in buffer pH‚ then greater intensity in
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The enzyme is unaltered at the completion of the reaction. In this stimulation the amount of product produced during the course of an enzymatic product produced during the course of an enzymatic reaction will be measured. Hypothesis 1: What is the estimate optimal of ph? Hypothesis 2: What is the estimate optimal of temperature? Hypothesis 3: How does the concentration of enzyme influence the rate of reaction? Why do you think this occurs? Materials ● ● ● ● ● ● ● Substrate solution Cuvette Spectrophotometer
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rate change over time? How does this compare to a real enzyme? The enzyme’s rate did change over time. This compares to a real enzyme because an enzyme’s job is to speed up the reactions and as time allotted. That did happen since the enzyme in our lab was able to make more chainobeads as time progressed. 4. Graph 5. Table Chainobead Construction Time Part A 15 Seconds 6 30 Seconds 12 60 Seconds 20 120 Seconds 29 Part 1B: 1. The results of the 120 seconds with the non-pop beads added to the mix
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Since the Grignard reagent can easily react with water‚ all glassware including the 25 ml round bottom flask‚ magnetic stir bar‚ 3 and 5 ml conical vial‚ 50 mL Erlenmeyer flask‚ claisen adapter‚ drying tube and 5 glass pasteur pipets were first added to a 250mL beaker and placed in the oven for 30 minutes. After the completion of the thirty minutes‚ 0.150 g of shiny magnesium turnings and a stir bar was first added to the round bottom flask and the claisen adapter along with the drying tube packed
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Bean Growth with Different pH Levels Through this experiment‚ the objective was to find which pH liquid created the best environment for the Mung beans to grow. First‚ the beans were placed into bags with different pH liquids. Then‚ measurements were taken each day to monitor the growth average of the beans. The pH 2 beans average size at the end of the experiment were 0.24 cubic centimeters‚ the pH 7 size average was 0.38 cubic centimeters‚ and the size average of the pH 2 beans was 0.24 cubic centimeters
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Lab 1: Heart Rate Lab (Revised Fall 2010) Lab 1. Heart Rate‚ Physical Fitness‚ and the Scientific Method Prelab Assignment Before coming to lab read carefully the following pages on the scientific method and then answer the prelab questions at the end of this lab handout. Be prepared to discuss and/or hand in your responses to the prelab questions at the start of lab. Introduction Biology is a dynamic field of study whose aim is to unravel the mysteries of life itself. Throughout history‚ humans
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The Effect of pH on Enzyme Activity A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of
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- Temperature (21°C) - Time (±0.01 sec. stopwatch was used to keep the buffer solution and FeSO4 with catalase for 30 seconds. Then the H2O2 solution was added and this was given a further 90 seconds) - Apparatus Materials: - Gloves - Safety goggles - Buffer solution pH 7 - Distilled water - Solution of H2O2 - 0M‚ 0.2M‚ 0.4M‚ 0.6M‚ 0.8M‚ 1M solutions of FeSO4 - Scalpel - Forceps - Petri dish - Balance (±0.01 g) - Stopwatch
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