Interpretation From the graph the enzyme Trypsin has 51% light transmission at pH 2. At pH 9 the Trypsin has 39% light transmission. Between pH 2 and pH 9 the percentage of light transmission decreases at a steady rate‚ until it reaches pH 8 where there is a steep increase from 30% to 39% as the enzyme has reached its optimum pH at 8. During the experiment in the boiling tube this pH had the deepest red colour as the most protein gelatine was broken up. This meant that in the colorimeter when testing
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determining the effects of pH on enzyme activity show that as the independent variable‚ pH‚ increases the dependent variable‚ percentage transmission‚ decreases. This is shown in the results as at the lowest pH‚ pH 4.0 the average percentage transmission is at its highest at 97%. At the highest pH‚ pH 8.0 the average transmission is 78.5%. This is also supported from the graph as it produces a negative gradient showing that as the percentage transmission will decrease with an increasing pH. This happens because
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Science Coursework Trypsin Investigation Aim I am going to prove that temperature effects the rate in which the enzyme Trypsin breaks down milk substrate into amino acids. Investigation "We strongly recommend the use of Vanish Stain Removal Tablets in conjunction with a 900 spin wash at 70°C" This was found on a milk carton of a well-known drinks company‚ Nesquick. The major food group constituent of milk is‚ of course‚ milk. For my Science Investigation‚ I am going to test different temperatures
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The enzymes meant to digest your food and nourish you turned on you and started eating your body instead. In your bloodstream was a virus that was very specific to Alpha-1 Antitrypsin‚ the substance that inactivates Trypsin‚ Chymotrypsin‚ and Neutrophil Elastase. I know that’s all Chinese to you but you’ll soon understand. I decided to play a game with you‚ the same way you played games with me. I decided to trick your body into thinking it was doing what it was supposed
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It is logical that if the enzyme‚ Trypsin‚ functions at the optimum salt concentration of 0.15M‚ then its ability to function efficiently will decrease. The rationale for this proposed relationship is that Trypsin won’t function in a different concentration than 0.15M because it performs best at that value. Anywhere else it may break down or work at a slower pace. MATERIALS & METHODS: To better understand the effect of salt concentration on trypsin the tubes were marked as 2mL with the different
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SBI4U Test A2 Table of Contents 1. What are the function roles and biological significance of the enzyme? Trypsin is part of the digestive system and degrades proteins‚ making it an enzyme known as protease. [1] It is one of the three principal digestive proteinases‚ the other two being pepsin and chymotrypsin. [9] Trypsin primarily hydrolyses peptides into smaller building-blocks‚ mainly amino acids (these peptides are the result of the enzyme pepsin ’s breaking
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hypothesis that if salt concentration is not equal to the optimum concentration of 0.15M‚ then its ability to function will decrease. 0.15M with the trypsin added had the lowest absorbency compared to the three other concentrated groups that were tested (0.0M‚ 0.075M‚ 0.30M). Closer comparison that if you take the results obtained from trial one with trypsin at a salt concentration of 0.075M‚ the absorbance was 0.165 (440 nm wavelength). In comparison to 0.15M‚ which had an absorbency of 0.108 (440 nm
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MEASURING pH Date of Lab: 02/09/13 Date Report submitted: 02/26/13 Purpose of the experiment By doing this experiment‚ we were trying to learn how to measure a pH with a pH meter to determine whether a solution is weakly‚ moderately or strongly acidic or basic; but also to compare the different data founded to see how the addition of certain liquid to a solution may affect the pH of the initial solution. Materials We used: * Tap water‚ distilled water‚ Soda and milk as the main
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The pH Scale is a scale made to show how acidic or alkaline a substance is. The pH of something is measured with pH paper which can vary from wide range paper‚ which covers all ph’s‚ but is not as accurate at some others. There are pH papers that get very specific that can have a range such as 2 or 3 and get much more precise. The neutral on the scale is 7. Anything that is below 7 is considered acidic and anything above 7 is considered alkaline. Acidic substances contain more H+ than OH-. H+
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A pH meter is an electronic instrument used to measure the pH (acidity or alkalinity) of a liquid (though special probes are sometimes used to measure the pH of semi-solid substances). A typical pH meter consists of a special measuring probe (a glass electrode) connected to an electronic meter that measures and displays the pH reading. The probe The pH probe measures pH as the activity of hydrogen ions surrounding a thin-walled glass bulb at its tip. The probe produces a small voltage (about 0
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