Investigating the effect of pH on the activity of the enzyme catalase. Introduction Hydrogen peroxide (H2O2) is a very pale blue liquid which appears colourless in a dilute solution‚ slightly more viscous than water. It is a weak acid. It has strong oxidizing properties and is therefore a powerful bleaching agent that is mostly used for bleaching paper. Catalase is a common enzyme found in all living organisms. Its functions include the conversion of Hydrogen Peroxide‚ a powerful and potentially
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compounds that generally have opposite characteristics. They have there own unique traits‚ acids taste sour‚ turn litmus red while bases taste bitter and turn litmus blue. The term pH relates to measuring the acidity or alkalinity of a solution‚ such as vinegar‚ or even a damp substance like soul. The neutral pH is 7‚ which is essentially water‚ with lower numbers indicating acidity and higher numbers indicating alkalinity. Hydronium ions (H3O+) are formed when water molecules (H2O) bond with
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The Effect of Concentration‚ pH and Temperature on Enzyme Activity Biology For Majors October 4‚ 2012 Abstract We examined the reaction an enzyme has when its concentration‚ pH and temperature are altered. In order to do this‚ we added different levels of pH into different test tubes with the enzyme (sucrose)‚ and substrate (sucrose)‚ and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature
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September 20‚ 2010 EXPERIMENT Acids‚ Bases‚ and Neutrals Problem Are common house-hold products pHs balanced and neutral? Information pH measures how acidic or alkaline a solution is. Its concept was introduced in 1909 by biochemist Soren Sorensen. The scale ranges from 0-14. Solutions that range from 0 to 6 are considered to be Acidic. Solution that’s on the Acidic end of the scale is low in ph‚ high in hydrogen ion‚ and low in hydroxyl ions. An acid have a sour taste‚ reacts with metal‚ are
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Observing Enzyme Activity Purpose: The purpose of this experiment was to test whether the pH affected the enzyme reaction rate. Hypothesis: If the enzyme is in a basic solution‚ then it will react faster because the enzyme (catalase) reacts better in basic solutions. Materials: 10 potato cubes (1 cm3) -Pipet Baking soda solution -50 ml glass beaker Bleach Water Lemon juice Vinegar 5 glass test tubes Drying rack Timer Graduated cylinder Hydrogen peroxide Procedure:
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professor! I chose to discuss topic 2 this week. The topic is Discuss how buffers help to regulate body pH. The pH level in the blood is maintained by the kidneys and the lungs. The pH level on the blood should be at 7.4. In order for the kidneys and lungs to maintain this pH level it affects the buffers in the blood. The buffer is a substance that is resistant to the change in the body’s pH level. Basically the buffers can make an acid or base less potent and try and neutralize it so the body is
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College General Biology I Enzymes‚ test for effect of pH on catalase activity Purpose The main purpose of this experiment was to learn about enzymes and how to test for the effect of pH on catalase activity and to be able to tell if a reaction is an exergonic or endergonic process. Introduction Enzymes are made from amino acids‚ which are made from proteins. In order to make an enzyme‚ hundreds of amino acids are strung together in a very specific and unique order and eventually is folded
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Experiment 1 : Design and preparation of buffers effective at different pHs Abstract The body uses natural buffers to maintain the many different pH environments in our body. This is important for optimum activity of our enzymes. When doing experiments in vitro using these enzymes it is important to mimic intracellular conditions using artificial buffer systems in order to obtain accurate results. In this experiment the buffering properties of three artificial buffer systems containing acetic
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AbstractDifferent pH level may affect the growth and development of the plants. Certain key words include: pH level‚ germination‚ acidity‚ osmosis and diffusion. This experiment examines the effects of different pH level of vinegar on the growth of bean plants. Materials used in this experiment consist of: water (pH 6)‚ vinegar with the pH level of 3‚ 4 and 5 (each one were made before experiment)‚ beans‚ soil‚ and pots. Eight bean plants were planted‚ two were watered with pH 6 and the other ones
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Investigation on Effects of Different pH on Enzyme Activity How does the different pH buffers affect activity of potato enzyme/extract? Introduction: Proteins are polymers that are made up of smaller units/monomers called amino acids. There are 20 different types of amino acids‚ thus make up many different combinations in types‚ numbers of amino acids as well as their orders – an explanantion for why there are so many proteins. Every protein‚ due to various reactions of amino acids to each
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