you cannot just say you did the experiments at the same time in the same room etc. You will need to monitor this using a thermometer. 2) pH: c. Independent: A range of pH should be used to establish the bell-shaped curve. Buffers should be used to do this. d. Control: You will need to use the same buffer (appropriate to the optimum pH of the enzyme used) for every sample. 3) Substrate concentration: e. Independent: Serial dilutions‚ or % concentrations (unless you
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two enantiomers and test each for its biological activity? 2. (6 pts) Phosphoric acid (H3PO4) has three dissociable protons‚ with the pKa’s shown below. Which form of phosphoric acid predominates in a solution at pH 4? Explain your answer. Acid pKa H3PO4 2.14 H2PO4– 6.86 HPO42– 12.4 3. (4 pts) Mutations may arise during DNA replication when mispairing occurs as a result of the
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the cells will start to divide and grow. [2] Enzymatic dissociation is the second method and is more commonly used. The enzymatic dissociation speeds up the explant culture by the addition of digesting enzymes. The digesting enzymes are usually trypsin or collagenase and they work by dissolving the tissue fragments and the cement holding the cells together. This creates a suspension of single cells that are placed into
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Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique
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quality products is more acute today. In this project I have prepared soy milk from soyabeans and have compared the conditions of formation of good quality cow milk yogurt and soy milk yogurt. I have also tried to find the effect of temperature on the pH of cow’s milk and soy milk. Thus I have selected this project to find a suitable replacement of cow milk in soy milk to meet the demands of the ever increasing population. SOY MILK: Soy milk (also called soya milk‚ soymilk‚ soybean milk‚ or soy
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their function is determined by their complex structure. The reaction takes place in a small part of the enzyme called the active site‚ while the rest of the protein acts as "scaffolding". This is shown in this diagram of a molecule of the enzyme trypsin‚ with a short length of protein being digested in its active site. The amino acids around the active site attach to the substrate molecule and hold it in position while the reaction takes place. This makes the enzyme specific for one reaction only
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PH 131 BIOCHM FNDTN THERAPEUTICS DR. JOSEPH 1. Select the predominant form of Amoxicillin (shown above) at pH 5.3. The pKa of the phenolic -OH is 9.6‚ the NH3+ is 7.4‚ and the COOH is 2.4. 2. At what pH would amoxicillin most readily cross a chloride channel? A. 1.8 B. 5.7 C. 8.0 D. 10.5 E. 3.6 3. Using the pK values shown in parentheses in the above diagram‚ select the pI of the tripeptide Arginiyltyrosinylcystyl from the following choices: A. 11.6 B. 5.0 C. 5.6
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Cucumis melo Linn. was first described by Linne in 1753. It belong to the family Cucurbitaceae which consists of 825 species in 118 genera (Milind and Kulwant‚ 2011). The birth of Cucumis melo L. has been uncertain. However‚ novel review has testified its origin from East and South Africa. Different cultivated varieties and different forms of fruits have emerged universally in tropical and sub-tropical regions. Cucumis melo L. has a wide geographic distribution. Native countries include South Africa
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Effects of long-term ketamine administration on rat bladder protein levels: A proteomic investigation using two-dimensional difference gel electrophoresis system Abbreviations & Acronyms 2D-DIGE = two-dimensional difference gel electrophoresis CHAPS = 3-[(3Cholamidopropyl) dimethylammonio] propanesulfonate Cy = cyanine dye DIGE = difference gel electrophoresis DTT = DL-Dithiothreitol GAPDH = glyceraldehyde 3-phosphate dehydrogenase HK = high dose of ketamine IC = interstitial
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2. Experimental 2.1. Experimental procedure for the synthesis of bone-like CS-PEG-HA-ZrO2 nanocomposites (BNC I-III). At first‚ ZrO2 nanoparticles (NPs) were synthesized by using the previously reported method [16]. Briefly‚ aqueous solution of sodium hydroxide (purchased from HiMedia Private Ltd.) (40 ml 0.05M) was slowly added in zirconium oxychloride octahydrate (purchased from Merck India Ltd.) solution (100 ml 0.01M) prepared in methanol-water (1:1) at 5 oC temperature. The mixture was stirred
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