Bio 6 (Mon. Lab) 5/6/13 The Effects of Pepsin VS. Trypsin in The Digestion of Protein Introduction As food is mechanically and chemically digested through our oral cavity then passes through our pharynx and down our esophagus‚ our food then enters the stomach. The stomach‚ being the main organ for storage‚ also helps with breaking down our food‚ but in order to accomplish that our pancreas helps by excreting an inactive digestive enzyme called pepsinogen. Pepsinogen is the inactive form
Premium
watch § Test tubes § Photographic film (b/w) § Syringe § Test tube rack § Ruler § Scissors § O.1% trypsin made in pH7 buffer solution § Mounted needle 2. I then decided on the range of results and temperatures I was to investigate. I decided to observe 20º‚ 30º‚ 40º‚ 50º‚ 60º‚ 70º and 80º. 3. Next‚ I label each test tube with the temperature to be investigated and then added 2.5cm3 of Trypsin solution. 4. I ensured that I cut the necessary pieces of film using a ruler and a pair of scissors
Premium Cell membrane Cell
An experiment to investigate the effect of temperature on the rate of reaction of the Enzyme Trypsin. Aim: This investigation was on the effect temperature has on the rate that the enzyme trypsin hydrolyses its substrate‚ a protein found in milk (casein). This investigation was conducted under controlled conditions‚ the temperature being the changeable variable. Trypsin and its substrate (powdered milk which is a source of the protein casein) were heated in a water bath. The contents of the two
Premium Enzyme
can reach a new pH level by interacting with soil. If water has a pH that is too high or too low‚ it can be life threatening to aquatic species and can corrode metal structures. After finding this out‚ I decided to test if water could reach an extreme pH just by interacting with soil around it. The question for this experiment therefore became the following. Will interaction with soil change the pH of water? I hypothesized that if water interacts with soil for three days‚ then the pH of the water will
Premium PH Acid Soil
This experiment shows the effects of changing the pH level has on Catalase. As predicted‚ the farther away the pH levels got from the optimum pH (7.2)‚ the lower the reaction rate. At a pH of 7.2‚ the foam of the reaction measures 6cm. At a ph of 3 it measures 2.5 cm‚ at a pH of 5 it measures 2.75 cm‚ at ph 9 it measures 2.3 and at 11cm it measures 2cm. pH measures the hydrogen ion concentration of a substrate. By changing the pH of the catalase‚ the enzyme was denatured. Denaturing is the result
Premium Enzyme Chemical reaction Catalysis
concept and meaning of pH 2. Provide the student experience in measuring pH a. pH testing paper 3. Test the student’s hypothesis as it related to the pH of common solutions Hypothesis The pH of the tested solutions will be in the order of the following according to a pH scale: 1. Lime juice 2. Orange juice 3. Soda 4. Iced Tea 5. Milk 6. Water 7. Soapy water Material Required To facilitate this laboratory exercise‚ the experimenter needs the following: pH strips Sample reservoirs
Premium PH
II: Titration with a pH Meter 1. Fill the 50-milliliter buret with a 0.25 molar NaOH solution. 2. Record volume. 3. Measure out between 20 milliliters and 40 milliliters of the unknown HCl solution. This amount must be different than the amount used in part I. 4. Record volume. 5. The amount of unknown HCl is then added to the 100-milliliter Erlenmeyer flask. 6. Insert the pH meter into the Erlenmeyer flask and record the initial pH of the acid. Remember to record the pH of the solution after
Premium PH Hydrochloric acid Titration
Properties of pH The manufacture’s claim is that Sensodyne toothpaste micro hardens tooth enamel to help protect against the effects of acid wear‚ helps stop the twinges of pain from sensitive teeth formulated with low abrasivity‚ neutral pH and gentle yet effective cleaning system. To find out if the manufactures claim on Sensodyne toothpaste is the correct level of pH. I expect the Sensodyne toothpaste to be a neutral pH level as the manufactures claim that this products pH level is neutral
Free PH
pH and Microbial Growth Introduction: Each organism has an optimum environment for growth‚ which includes pH level. Organisms that grow at near neutral pH levels are called neutrophiles. Those that grow best in acidic pH values are called acidophiles. Others‚ yet‚ grow best in alkaline pH values and are called alkaliphiles. In this lab‚ each group was assigned an organism‚ which was then used to inoculate 6 different pH levels. Materials and Methods: Followed lab procedure 29‚ “pH and
Premium PH Bacteria
PHILIPPINES TO LEAPFROG TO BE THE 16TH LARGEST ECONOMY BY 2050 ACCORDING TO IMF The Republic of the Philippines is one among those list of developing economy and owns the possibility of being one of the fruitful nation in the next succeeding decades. Our country is highly expected to jump 27 places to become the 16th largest economy by the year of 2050 as projected by The Hong Kong and Shanghai Banking Corporation International Bank. Resounding softly across the 7‚100 islands that form the Philippines
Premium United States