litmus paper and the pH probe determined the salt solutions’ pH levels. When the blue litmus paper was dipped into ammonium chloride‚ the paper was changed to red whereas the red litmus paper stayed the same indicating that the solution was acidic. PH levels shown through pH probe also ranged between 5.5 to 6.0. Yet when in the presence of sodium acetate blue litmus stayed blue whereas red litmus turned blue‚ indicating its basicity. pH probe also showed the solution’s pH varied between the line
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build large complex molecules like the enzymes that make DNA and enzymes also help cells to communicate with each other. I have decided to use not one but two enzymes to observe what happens to the different kind of enzymes when introduced to different PH (power of hydrogen) scales. the best way to test this was to put the enzyme bromelain in gelatine because bromelain affects gelatine. If added to the gelatine‚ the bromelain in pineapples will prevent the gelatine from setting. However‚ different circumstances
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resists (or buffers) a change in its pH. That is‚ we can add a small amount of an acid or base to a buffer solution and the pH will change very little. How to calculate pH of buffer solution containing both acid and conjugate base? Dissociation constant definition 1.1 can be rearranged into or (note that due to sign change [A-] was moved to nominator). This is so called Henderson-Hasselbalch equation (or buffer equation). It can be used for pH calculation of solution containing
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strong base plotted against the pH .The pH is an indicator of an acids strength. The titration curve can be used to determine the pKa. By reading the graph the equivalence point can be found; which is the point where equal parts acid and base have reacted by knowing this the half-equivalence
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seconds‚ pH = 7.4 2. At 40 seconds‚ pH = 7.4 3. At 60 seconds‚ pH = 7.4 4. Did the pH level of the blood change at all during normal breathing? If so‚ how? No 5. Was the pH level always within the “normal” range for the human body? Yes 6. Did the PCO2 level change during the course of normal breathing? If so‚ how? No Activity 2a: Hyperventilation – Run 1 1. At 20 seconds‚ pH = 7.47 2. At 40 seconds‚ pH = 7.52 3. At 60 seconds‚ pH = 7.67 4. Maximum pH = 7.68 5. Did the pH level of
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Hypothesis: If enzyme activity is affected by the pH of a solution‚ then the enzymes will experience the greatest activity at a pH of 6. Variables: Independent Variables Dependent Variable Controls Four different pH values (10‚ 7‚ 6‚ and 3) Change in color of the solution The amount of potato extract‚ pH solution‚ and catechol used (1 cm +/- .1cm) Size of the test tubes Amount of time allowed for the catechol to sit with the potato extract and pH solution (20 minutes with 5 minute intervals
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Page 20 Acids and bases Unit plan Grade 12 General Objectives: ▪ Introduce pH‚ acid and base definition ▪ Discuss acid‚ base‚ strong acid‚ weak acid‚ aqueous‚ neutral‚ pH definitions. ▪ Introduce Kw the ion product of water. ▪ Introduce Kw the ion product of water ▪ Explain through Kw why solutions with pH less than 7 are acidic and why those with pH greater than 7 are basic. ▪ Define volumetric analysis‚ titration‚ standard solution‚ equivalence
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Effects of Temperature‚ pH‚ Boiling‚ and Hydroxylamine on the Enzyme Peroxidase Extracted From Brassica rapa Abstract In this experiment the enzyme peroxidase was extracted from from a turnip‚ Brassica rapa‚ and tested under different conditions. The effects of temperature‚ boiling‚ pH‚ and a competitive inhibitor were tested. The enzyme was tested at temperatures of 4°C‚ 24°C‚ 32°C‚ and 48°C. As the temperature increased‚ so did the activity of the enzyme
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Background Information Part 1 In the first part of the enzyme lab‚ we mixed a substrate and an indicator with an enzyme. There was also a neutral buffer in each of the chemical mixtures. The neutral buffer regulated the pH to around 7. We got a color palette and once we mixed each together‚ we observed and saw a change in the color of the substance. The darker and more brown the substance got‚ the more oxygen produced by the reaction. Our results showed that amount of oxygen produced increased
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The Effect of pH on Enzymatic Reaction Rate Abstract Enzymes are affected by changes in pH. The most favorable pH value— the point where the enzyme is most active—is known as the optimum pH. This experiment was conducted to determine the effect of pH reaction rate on an enzyme‚ catalase‚ from yeast. The experimental results indicate that the catalase worked best at a neutral pH level of seven (7). Introduction An enzyme is a protein molecule that serves as a catalyst. “The basic function of
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