Collins Instructor: Catherine Rice Date: 07.09.2014 Predictions Sucrase will have the greatest activity at pH 5 Sucrase will have the greatest activity at 70 °C (158 °F) Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount of substrate (sucrose) present‚ sucrase + sucrose incubation time
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Weekly iLab iLab: Exploring the Microbiology Lab Section 1 Lab Safety 1. There are four safety equipment items that a lab should have. Identify two of these four items. (2 pts) Biological Safety Cabinet‚ Eyewash and shower 2. Identify one of the three ways to keep your work area safe. (1 pt) Keep your workspace free of all unnecessary materials 3. There are five recommendations for dressing properly in a lab environment. Name two of these recommendations. (2 pt) Avoid loose
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molecule accepts a proton from the other – see p 532 - equilibrium of the reversible reaction – can write the constant - Kw = [H +] [OH –1 ] = (1.0 x 10 –7 )2 = 1.0 x 10 –14 - Kw changes at different temp ( remember concept from ch 7 ) - so pH of pure water changes with diff temp - but pure water always neutral since # protons = # hydroxide ions Strong Acids - ionize more than 99 % in water ex: HCl - monoprotic acids – have only one ionizable H ex: HNO3 p 537 practice # 4‚ 5‚ 6.
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Naguilian National High School Library in terms of hardware‚ software‚ peopleware and network. Table 2. Information and Communication Technology Profile of Naguilian National High School in terms of Hardware‚ Software‚ Peopleware‚ Network. Indicator | Status | A. Hardware | | 1. Computing devices by | | Desktop PC | Existing | Printer | Existing | Router | Existing |
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reaction. During complex formation experiment‚ student needs to observe the different reaction occur when adding ammonia or sodium hydroxide in small quantity and in excess. At the second part‚ student needs to measure the pH of different solution by using universal indicator paper and determine whether each solution is acidic‚ basic or neutral. Lastly‚ the precipitation reaction is observed by using phosphates‚ chlorides and silver halides groups. AIMS The main objective of this experiment was
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PNP PNP or paranitrophenol is an organic compound that undergoes changes when the pH is manipulated of the solution. PNP is also an acid/ base indicator. The compounded starts off as internal conjugated ring with the hydroxyl group and the nitro group single bonded to the aromatic ring. This occurs at the pH of 5. At this point the aromatic compound is colorless and is in the ultraviolet range. However‚ as the pH begins to increase the hydroxyl group deprotonates‚ leaving the oxygen with a negative
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ethanoic acid (acetic acid)‚ CH3COOH • universal indicator solution • limewater (calcium hydroxide‚ Ca(OH)2) Marble chips (calcium carbonate‚ CaCO3) Copper(II) oxide‚ CuO Small pieces of magnesium‚ Mg Measuring cylinder 10 clean‚ dry test tubes Test tube rack Two spatulas Plastic forceps Dropping pipette 2 × 100 mL conical flask One-holed rubber stopper with bent glass delivery tube Retort stand 2 × Clamp and bosshead Long taper Box of matches pH colour chart Residue bottles Put on your lab coat
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is the indicator phenolphthalein in an acidic solution and in a basic solution? (2 K/U) Phenolphthalein is colourless in acidic solutions and pinkish in basic solutions. 2) Why does the clear colour‚ which forms at the point where the hydrochloric acid comes into contact with the solution containing phenolphthalein and base in the receiving flask‚ disappear more slowly near the endpoint? (2I) At the start of the titration‚ the NaOH solution in the flask will be pinkish and the pH is quite
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1. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity? The product used is‚ glucose and fructose‚ this is an indicator of sucrase activity because the amount of product produced is a result of the experiment. 2. Explain why denatured sucrase was used as a control? Denatured sucrase was used as a control because it increases the activity. 3. Alkaline DNS denatures sucrase. Explain why it is important to denature the enzyme? Denaturing the
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Biology- Change in pH Aim: To investigate the affect that change in pH levels has on a particular enzyme‚ in this case amylase. Hypothesis: In this investigation I expect as the pH reaches the optimum level‚ the rate of reaction will be fastest‚ compared to other pH levels. It is also suspected that after the enzyme has reached optimum level the enzyme activity will decrease. Through further study of the optimum level of amylase I found that the enzyme usually has an optima pH of 8. It is known
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