Determination of Florfenicol in Catfish by UV-mediated High Performance Liquid Chromatography (HPLC) 1. Introduction 1.1 Description of the Analyte Florfenicol (C12H14Cl2FNO4S) is a derivative of thiamphenicol. Its structure is identical to that of chloramphenicol – a synthetically developed antibiotic for veterinary use‚ such as treating a wide range of bacterial infections (Hayes p. 7). It is commercially available as 50 percent of Aquaflor® premix‚ which has been approved for the control of bacteria
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Adol Condensation Introduction: This reaction is carried out by adding benzaldehyde and acetone into a flask. The product created is a 1‚5-diphenyl-1‚4-pentdiene-3-one‚ which includes two double bonds‚ and two benzyl ring functional groups. This is a dehydration reaction that occurs twice in order to form the diene. After obtaining the product‚ via vacuum filtration‚ it will be recrystallized and then analyzed for purity by determining both products’ melting point. The two products will be
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The Effects of Individuals’ Detection of Changes in Images on Reaction Time Abstract The purpose of this current study was to examine how individuals detected or noticed change when viewing images on their level of attentiveness. The experiment consisted of 22 participants who had to detect change across conditions for 20 minutes. These conditions were importance of change (marginal and central) and change type (color‚ location and disappearance and reappearance of images). These
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Boys’ Code of Conduct In the article “How Boys Become Men”‚ written by John Katz‚ he analyzes situations he experienced while growing up. Katz believes that boys become men by not showing fear or feelings and not ratting on others. As in the novel‚ The Other Wes Moore‚ both Weses are forced to become men at an early age. They experience events that push them to make decisions that make them grow and think like men do. Katz details the maturation process for boys‚ as the book‚ The Other Wes Moore
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Transformation of E. coli by plasmid DNA 1. Table showing the results from the selective plates |Plate |Plasmid |Contents of plates |Number of colony | | | | |White |Blue | |1 |Ligation mixture |Ampiclillin + X- gal + IPTG |10 |0
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Determination of presence of protein in a variety of food substances by the use of Biuret Reagent Biol. 1040 Section 1005 Introduction The human body consumes carbohydrates‚ lipids‚ and proteins each day by eating a variety of foods. These foods are required for energy and human growth. Proteins are a diverse group of macromolecules with many different functions (Mbuthia‚ 2012). Many are structural components of bone‚ muscle‚ hair‚ tissue‚ etc. Others are enzymes that speed up cellular
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Purpose To check whether mass is gained or lost during a Chemical reaction. Hypothesis Equipments and Materials • Eye protection • Test tube • 250 ml Erlenmeyer flask and stopper • Weight Balance • Antacid tablet • Dilute solutions of o Sodium Hydroxide‚ NaOH(aq) o Iron(III) Nitrate‚ Fe(NO3)3 • Funnel • Cylinder Procedures 1. Put eye protection on for safety purposes. Part A: Reaction between Iron(III) Nitrate and Sodium Hydroxide. 2. Take two cylinders and fill one (full)
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Biosphere Lab Report A biosphere can be defined a lot of different ways. Our interpretation of a biosphere is the area in which life can be contained within. In the case of the earth‚ it means as high as a bird can fly and as deep as fish can swim in the ocean. A biosphere is a closed system that has a constant flow of energy between the organisms living within it and it needs nothing outside of itself but sunlight to continue to be sustained. At the University of Arizona‚ they attempted to build
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Chromatography lab Purpose: To separate food colorings into their component dyes using paper chromatography. Materials: Chromatography paper‚ Food coloring‚ Ruler‚ Pencil‚ Solvent solution‚ Test tubes‚ Test tube rack. Safety precaution: wear aprons‚ to make sure that you don’t get any of the alcohol on your clothes‚ and if you break a test tube you don’t get glass on you. Procedure: See-attached handout. Results: See chromatography with Audrey’s lab report.
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Purpose: To find out the densities and to find out the name of the unknown metals. (Based on the extensive and intensive properties) Check up the words mass‚ volume‚ density‚ extensive properties‚ and intensive properties. Where do the units for mass and volume) come from and what do they mean? What is the density of distilled water? What is Archimedes principle? Does temperature affect the density of a solid? Liquid? Gas? Materials: Safety glasses‚ 10‚ 25 or 50 mL graduated cylinders
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