Definition of Enzyme Unit and Specific Activity- 6 3.5 Determination of pH Optimum 6 3.6 Determination of Michaelis-Menten Constant 7 3.7 Ammonium Sulphate Fractionation 7 3.8 Gel Filtration on Sephadex 7 3.9 Chromatography on DEAE-cellulose 7 4 Results 8 4.1 Freshly Prepared Crude 8 4.2 Crude Thawed after a Week of Freezing 9 4.3 Sephadex 25 Gel Filtration 11 4.4 Determination of Optimum pH 12 4.5 Ion Exchange Chromatography 13 4.6 Michaelis-Menten Kinetics 13
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CROATICA CHEMICA ACTA CCACAA 78 (1) 99¿103 (2005) ISSN-0011-1643 CCA-2984 Original Scientific Paper Acid Value Determination in Vegetable Oils by Indirect Titration in Aqueous-alcohol Media Elena Kardash* and Yakov I. Tur’yan National Physical Laboratory of Israel (INPL)‚ Givat Ram‚ Jerusalem 91904‚ Israel RECEIVED JANUARY 16‚ 2004; REVISED JULY 21‚ 2004; ACCEPTED OCTOBER 28‚ 2004 Keywords acid value vegetable oils indirect titration A new method of acid value determination in
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Biology 20 IB Design Lab Does increasing or decreasing the ph level of a 3% concentration H2O2 (l) solution affect the amount of oxygen released between 15.0ml of the solution and a 5g sample of liver? The reaction is measured by the difference in mass of a balloon that is used to capture the amount of gas released by the catalase enzyme found in liver reacting with a basified 3% H2O2 (l) and an acidified 3% H2O2 (l) . Chemical reactions occur when two or more molecules interact and the molecules
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are true or not. Another fact which inspired me to do this project is that I am in touch with qualitative analysis whose knowledge with other factors helped me to do so. CONTENTS Ø Introduction Ø Theory Ø Apparatus Ø Chemicals required Ø Detection of pH Ø Test for Carbon Dioxide Ø Test for Glucose Ø Test for Phosphate Ø Test for Alcohol Ø Test for Sucrose Ø Result Ø Conclusion INTRODUCTION The era of cold drinks began in 1952 but the indianization of industry marked its beginning with launching of
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The Effect of the Type of Building Material on the pH of the Vinegar Introduction: For this experiment‚ the town council wanted to test which building material would be the best to build statues in a zoo. The building material with the lowest pH would be the best building material because while 7 is neutral anything below 7 is considered acidic and anything higher than 7 results in a base. Precipitation of 5.5 or lower is considered acid rain. Connecticut and most of the United States have to
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Determining the habitat preference of Artemia franciscana by creating artificial gradients of three abiotic factors: light‚ pH‚ and temperature Introduction In the study of ecology‚ species are often described as generalists if they live in broad environmental conditions or specialists if they live in narrow or specific environmental conditions (Reece et al. 2011). Artemia franciscana‚ the organism under study‚ is a well known generalist that has adapted it’s reproductive strategy to allow
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CHEMISTRY-WINE MAKING | Investigating the effectiveness of common fining agents on homemade fruit wine with respect to Turbidity‚ Sediment level‚ Ph and Alcohol content. | | Year 12 Chemistry | Extended Experimental InvestigationA comparison of chemical flocculation agents | Mario Mitov | Mrs Cullen 2011 | | Contents: 1.0- Abstract 2.0- Introduction/Background
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which point should the titration be stopped and the volume of titrant used be recorded? Answer : when the solution reaches the endpoint Question = Buffers are substances‚ or mixtures of substances‚ that (help maintain a relatively constant pH when small amounts of acids or bases are added) Question= Which of the following statements best describes what determines whether an acid is a strong acid or a weak acid? (the degree to which it dissociates in water) Question = The
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Biology- Change in pH Aim: To investigate the affect that change in pH levels has on a particular enzyme‚ in this case amylase. Hypothesis: In this investigation I expect as the pH reaches the optimum level‚ the rate of reaction will be fastest‚ compared to other pH levels. It is also suspected that after the enzyme has reached optimum level the enzyme activity will decrease. Through further study of the optimum level of amylase I found that the enzyme usually has an optima pH of 8. It is known
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This was done by spotting each of the 5 drop samples from each pH level‚ with each pH level being on a different piece of paper. Then the spot was allowed to dry and sprayed with ninhydrin to test for the presence of amino acid with gentle heat applied from hot plate. TLC of Amino Acid Fractions: Another TLC plate was spotted comparing the most concentrated fraction from each of the pH levels with the unknow mixture. The TLC developing jar was prepared with 4:1:1 propanol/acetic
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