Objective of experiment * The objective of the experiment is to assess the effect of different pH conditions on enzyme activity. Hypothesis * I feel the pH affected the enzyme activity to produce product. Prediction * If the pH affected the enzyme activity to produce product‚ then rate of reaction will change. Procedure * The procedure that we followed was to take two test tubes and label one pH2 and one pH10 and incubate 1 ml of each solution for 30 minutes. Then after 30 minutes
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catalyzes the synthesis of a large molecule from two smaller molecules. (4 marks) 9. Explain how the following environmental factors affect enzyme activity: a. pH (2 marks) b. Enzyme and substrate concentration (2 marks) 10. Examine the following data for three enzymes (A‚ B‚ and C). Each has been tested at a number of temperatures and pH levels to measure their activities. Based on these results‚ for each enzyme (A‚ B‚ and C)
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2008). Soil PH directly affects the life and growth of a plant‚ since it affects the availability of the nutrients needed for plant life. Most nutrients are in their most available state within a PH of 6.0 – 6.5 (Kissel and Miller‚ 2008). To germinate‚ seeds need favourable external conditions as well. In this lab the effect of acidic conditions on seed germination using lettuce seeds as the model has been observed. These properties were investigated using acidic solutions with various PH values. However
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In natural water‚ the pH can vary greatly based on the contents of the water. The pH test is important in relation to the the effects of hydraulic fracturing due to the chemical contents of the fracturing fluid. When these chemicals are mixed with any water solution‚ they cause the pH to increase greatly due the acidity of the chemicals. This can have a disastrous impact on the organic matter living the
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Determination of Percent Potassium & Percent Iron in an Iron Oxalate Salt by Ion Exchange Introduction: This experiment involves determining both the percent potassium (K) and iron (Fe) in a single titration after passing a solution containing a known mass of complex salt through an ion exchange column. Ion Exchange: Certain materials called ion exchange resins consist of rather large molecules which contain ions that can be displaced. The resins are solids‚ insoluble in water‚ usually granular
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Abby Kong Acid rain on plant -Background information Unpolluted rain is normally slightly acidic‚ with a pH of 5.6. Carbon dioxide (CO2) from the atmosphere dissolves to form carbonic acid. Acid rain is rain that has been made acidic by certain pollutants in the air. Acid rain is a type of acid deposition‚ which can appear in many forms. Wet deposition is rain‚ sleet‚ snow‚ or fog that has become more acidic than normal. Dry deposition is another form of acid deposition‚ and
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and the equivalence point of 8.4‚ as well as the value of pH. Purpose: The purpose of the experiment was to utilize the techniques of titrations to measure the concentration of an acid or base in solution‚ to calculate molar mass of an unknown acid or base‚ and to determine the equilibrium constant of a weak acid or weak base. Materials: NaOH Unknown acid Distilled water Balance Erlenmeyer flask Funnel pH sensor or pH meter Ring stand and buret clamp 250-mL beaker 50-mL
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Collins Instructor: Catherine Rice Date: 07.09.2014 Predictions Sucrase will have the greatest activity at pH 5 Sucrase will have the greatest activity at 70 °C (158 °F) Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount of substrate (sucrose) present‚ sucrase + sucrose incubation time
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Temperature‚ pH and oxygen requirement. Student: Temirlan Aitbekov Lab partner: Kanat Sadykov Instructor: Alessandra Clementi‚ MD‚GP Lab date: 7/11/14 Due date: 14/11/14 Nazarbayev University Abstract: This experiment is directed to investigate the various physical factors affecting the growth of microbes: temperature‚ pH of the
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Name: [Insert Your Name Here] Complete the following questions and submit your report. This document contains 4 pages. The total lab is worth 20 points. Purpose: The purpose of this lab is to demonstrate how temperature‚ substrate concentration‚ and pH can impact the rate of an enzyme reaction. In this experiment‚ you will use the Scientific Method to test your hypothesis. The enzyme used in this experiment is Invertase. This enzyme catalyzes the breakdown of sucrose (table sugar) into glucose and
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