other diseases (Babar et al‚ 1988; Oliveira et al‚ 1999). Thus‚ the effect of various conditions on trypsin activity is of great interest and is further investigated here. The effects of enzyme and substrate concentrations‚ as well as the effects of pH‚ on the initial rates of BAPNA hydrolysis by trypsin are investigated. Additionally‚ the inhibitory activity of the Jack Beans trypsin inhibitor is determined. This type of protease inhibitor in plants (particularly seeds) is widespread and well studied
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determine the identity of this unknown acid. Both values were found by preparing the unknown acid solution‚ preparing a buret‚ titrating the acid solution with the strong basic NaOH solution from the previous experiment‚ and determining the pH at various points using the pH meter. The formula weight of our unknown acid was found to be189.93 g and the pKa was 7.2 and 7.05 using two different methods. We were able to conclude that our proposed identity of our unknown acid was MOPS‚ which had a formula weight
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Respiratory Case Study The following case study is of a 37-year old Hispanic male weighing 145 lbs and 70 inches tall found unconscious by his girlfriend. According to her he was unconscious for about 15 hours and she was concerned because he would not wake or respond and was breathing shallow and slow. She then called 9-1-1. The patient entered the ER by emergency vehicle and on my initial assessment Pt had an altered mental status‚ was very unresponsive showing symptoms of a possible drug overdose
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WEEK 2 Written Assignment I’m thinking no more than 1-2 paragraphs each for 3 & 4. 1. List the qualities (at least 6) that define life AND discuss how a single-celled organism‚ such as an Amoeba or a yeast cell‚ and a more complex one‚ such as a tree or a cat‚ matches up with each characteristic 1. Living things have cells. 2. Living things grow 3. Living things reproduce 4. Living things respond to stimuli 5. Living things use energy 6. Living things adapt to their environment A single celled
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Alterations in Fluids‚ Electrolytes‚ and Acid-Base Balance Composition & Compartment Distribution of Body Fluids ← Body fluids are distributed between the intracellular fluid (ICF) and extracellular fluid (ECF) compartments. The ICF compartment consists of fluid contained within all of the billions of cells in the body. It is the larger of the two compartments‚ containing approximately two thirds of the body water in healthy adults. ← The remaining one third of body water is in
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solution? Materials: 1 M copper (II) nitrate 3 M hydrochloric acid 2 M sodium hydroxide 600 mL beaker 250 or 400 mL beaker Ice water Distilled water Stirring rod Bunsen burner Ring stand Al wire‚ 18 gauge or heavier Graduated cylinder pH paper Watch glass‚ 90 mm Goggles Acetone Procedures: Conversion 1: THIS IS ALREADY COMPLETED FOR YOU!!! Copper reacts with nitric acid to produce a brownish-orange gas called nitrogen dioxide. The blue color of the solution is characteristic
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water bath and measuring the desired temperature using a thermometer. PH: I will make sure that the pH is stable (constant) and only the temperature varies this is to be done by using buffer in every test tube so as to maintain pH balance for each beetroot sample and insure that pH does not become a variable. PH is important for maintaining the integrity of the cell membrane as integral proteins can denature upon change in pH. Also‚ presumably the buffer will have the right concentrations of salt
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Determination of the Equilibrium Constant for Ferric Thiocyanate In this laboratory exercise‚ the equilibrium constant(Kc) for the reaction between the Ferric Ion (Fe3+) and the Thiocyanate Ion (SCN-) was determined. After measuring the absorbance of the Ferric Thiocyanate‚ and carrying out some calculations; it was determined that Kc=130.81M Introduction: Chemical equilibrium is established when a reaction exists in a state where the forward reaction rate is equal to its reverse reaction
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Date: 07/27/14 Total Score: 100 % 1 CATEGORY anatomy QUESTION Backward effects of left-sided heart failure include: CORRECT ANSWER A) Pulmonary congestion YOUR ANSWER A) Pulmonary congestion Deoxygenated blood from the right ventricle flows through the right and left pulmonary arteries to the right and left lung. After entering the lungs‚ the branches subdivide‚ finally emerging as RATIONALE capillaries which surround the alveoli and release the carbon dioxide in exchange for oxygen. The capillaries
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A. 50 mM Potassium Phosphate Buffer‚ pH 6.5 at 25° C (Prepare 50 ml in deionized water using Potassium Phosphate‚ Monobasic‚ Anhydrous‚ Sigma Prod. No. P5379. Adjust to pH 6.5 at 25° C with 1 M KOH.) 1 mM L-Tyrosine Solution (Prepare 100 ml in deionized water using L-Tyrosine‚ Free Base‚ Sigma Prod. No. T-3754.) Tyrosinase Enzyme Solution (Immediately before use‚ prepare a solution containing 500 - 1‚000 units/ml of Tyrosinase in cold Reagent A.) T = 25° C‚ pH = 6.5‚ A280nm‚ Light path = 1 cm Continuous
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