Enzyme Kinetics Examples and Problems 1. An enzyme is produced for producing a sun protection lotion. Given kinetic data for the enzyme reaction with Vm=2.5 mmol/m3.s‚ Km=8.9 mM and So=12mM‚ what would be the time required for 95% conversion in a batch reactor? 2. An enzyme was assayed at an initial substrate concentration of 10-5M. The Km’ for the substrate is 2x10-3M. At the end of 1 min‚ 2% of the substrate had been converted to product. a. What % of the substrate will be converted
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Title: Kinetics: The Rate of a Chemical Reaction Objectives: 1. To study the kinetics of chemical reaction‚ 2 I- + S2 O82- I2 + 2 SO42- . 2. To study the effects of reactant concentration (persulphate‚ S2O82-‚ and iodide‚ I-) and temperature on the rate of chemical reactions. ( i) Study the effect of 0.20M (S2O82-) on the rate of chemical reaction. ( ii) Study the effect of 0.10M (S2O82-) on the rate of chemical reaction. ( iii) Study the effect
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This assignment discusses chemical kinetics by determining the rate and average value for rate constant based on experimental values given. The orders of the reactants can only be determined experimentally‚ so we must look at how the concentration of the reactants affect the instantaneous initial rate while holding the concentration of the other reactant constant. In this reaction‚ the overall reaction order for the rate law is third because the order for A is second and the order for B is first
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types of forces involved. Two of the main forces experienced are called potential energy and kinetic energy. Potential energy is the energy an object has depending on the position of it. For example the higher an object is the higher the potential energy is and the lower an object is the less potential energy it will have. Kinetic energy is also a main type of force that is involved with rollercoasters. Kinetic energy is the energy an object will have as it is in motion. There are many different types
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momentum of the object over a long time D. Increase the momentum of the object over a long time 8. Most street barriers are made of hard rubber or plastic materials that easily give in or dent when hit. Why is this so? A. To reduce the vehicle’s kinetic energy when it collides with the barriers B. To store potential energy in the bumpers of vehicles C. To reduce the impact force when hit by a vehicle D. To reduce the impact time when the barriers hit the vehicle 9. What is the momentum of a 20
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microbiology of biological phosphorus removal in activated sludge systems Robert J. Seviour à ‚ Takashi Mino‚ Motoharu Onuki Institute of Environmental Studies‚ Graduate School of Frontier Sciences‚ University of Tokyo‚ 7-3-1 Hongo‚ Bunkyo-ku‚ Tokyo 113‚ Japan Received 11 December 2002; received in revised form 18 February 2003; accepted 20 February 2003 First published online 20 March 2003 Abstract Activated sludge systems are designed and operated globally to remove phosphorus microbiologically‚
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Pablo Cuate Biochemistry Lab Report Objective: The purpose of this lab is to analyze enzyme activity at pH neutral for acid phosphatase as the colorless p-nitrophenylphosphate (PNPP) substrate concentration changes. We will empirically determine the concentration of production formation‚ Vmax and Km from absorbance of yellow PNP in the reaction. Results Figure 1 This figure indicates a positive linear trend for the concentration of PNP and absorbance. Table 1: Absorbance Value for Acid Phosphatase
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Abstract To determine the rate law with respect to bleach and blue dye‚ a series of graphic and algebraic manipulations were done on the data obtained in the kinetic trace experiment in order to determine the different components that made up the rate law. Using absorbance spectroscopy to monitor concentration over time‚ rate order of the dye was found to be 1st order through the integrated rate law and through the proportionality method; the order of the bleach was also determined to be 1st order
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Another postulate of the kinetic molecular theory is that gas particles are always in motion‚ like the other states of matter. But they are different in that they undergo random translational movement. In solids‚ the particles mainly experience vibrational motion and in liquids they mainly vibrate and rotate‚ with some translational motion. Gas particles move rapidly in straight lines‚ unless acted upon by another particle or the walls of a container. This continuous contact with the container leads
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Chemistry 12: Reaction Kinetics Review 1. Looking at the expressions for reaction rate‚ write expressions with which you could express rates for the following reactions. (Hint: look at what happens to reactants and products.) Recall that solid or liquids can lose or gain mass‚ gases can lose or gain volume and aqueous solutions can increase or decrease in concentration. ("a" is done as an example.) a) Mg(s) + 2HCl(aq) H2(g) + MgCl2(aq) reaction rate = mass of Mg consumed unit time b) c) or
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