so‚ a problem was encountered. While measuring the absorbance using Bromophenol Blue‚ it was discovered that the liquids leaked out into the compartment. After this was cleaned up‚ it became evident that the problem arose due to a zeroing of the colorimeter that was too forceful. This error was corrected and the experiment then continued smoothly. Discussion A very important step in the course of both experiments was to zero the spectrophotometer using distilled water. This was done in order to
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Soxhlet extractor From Wikipedia‚ the free encyclopedia Jump to: navigation‚ search A schematic representation of a Soxhlet extractor 1: Stirrer bar 2: Still pot (the still pot should not be overfilled and the volume of solvent in the still pot should be 3 to 4 times the volume of the soxhlet chamber) 3: Distillation path 4: Thimble 5: Solid 6: Siphon top 7: Siphon exit 8: Expansion adapter 9: Condensor 10: Cooling water in 11: Cooling water out Animation describing mechanism of Soxhlet
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and Castellucci 2000). Examples are the introduction of lasers‚ Fourier transform (FT) Raman‚ charge-coupled device (CCD) detectors‚ confocal Raman spectroscopy and fiber-optics‚ that allowed conservation scientists to record molecular spectra of minute samples or directly from the art object. Case study Red pigment samples were collected from the early Christian necropolis of El-Bagawt at Kharga Oasis‚ Egypt. El-Bagawat necropolis is dating back to the 4th century AD. Two of the most outstanding
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50-mL Sample of consumer sports drink‚ 10-mL Water‚ distilled or deionized Beakers‚ 50-mL‚ 2-3 Cuvets or test tubes‚ 13 x 100 mm‚ 3-8 Kimwipes or lens tissues Pipet‚ serological‚ 10-mL Pipet bulb or pipet filler Spectrophotometer or colorimeter Test tube rack Procedure: 1) Turn the spectrophotometer on allow to warm up for 15-20 minutes. 2) Based on maximum absorbance of the dye tested‚ select the appropriate wavelength on spectrometer. 3) Read the entire procedure. Construct an
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The Visible Spectra Of Orange Soda Pop Objective : To measure an absorbance value of the different colour solutions by using Spectrophotometry Introduction Spectrophotometry involves the use of a spectrophotometer. A spectrophotometer is a photometer (a device for measuring light intensity) that can measure intensity as a function of the color‚ or more specifically‚ the wavelength of light (nm). The instrument also can be use on biological sample such as chlorophyll pigments and
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results to which means that no achromatic color was used. If black‚ white or grey were used plus another color other than blue‚ then the results could’ve been more accurate and provided more explanations. Also‚ the colors were not verified by a colorimeter‚ instead the color properties were controlled by the software program. The sample was also very small. There was only 58 participants. If a greater sample was used‚ the results could’ve been different. Other then limitations‚ the study had it’s
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TEXT BOOK OF BIOCHEMISTRY For 2nd year Medical Laboratory Technician Course (INTERMEDIATE VOCATIONAL) Author : S. Srinivasa Rao‚ B.Pharmacy‚ Jr. Lecturer in M.L.T.‚ Alluri Sri Rama Raju Govt. Junior College‚ Shanthi Nagar‚ Khammam. Editor : S. Kamalakar Rao‚ M. Pharmacy Senior Lecturer in Pharmacy‚ Department of Biotechnology‚ Govt. institute for post diploma courses in engineering and technology‚ Ramanthapur‚Hyderabad. INDEX Chapter I. Page No. Instrumental methods of Biochemical
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All surface water and some groundwaters require treatment prior to consumption to ensure that they do not represent a health risk to the user. Health risks to consumers from poor quality water can be due to microbiological‚ chemical‚ physical or radioactive contamination. However‚ microbiological contamination is generally the most important to human health as this leads to infectious diseases which affect all populations groups‚ many of which may cause epidemics and can be fatal. Chemical contamination
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Unit 3. Assignment 1: Bacterial Growth Rates 1. Mediums that could be used to determine shigellosis include Btilliant Green Agar‚ and Triple Sugar-Iron Agar. Expected results in a confirmed case of shigellosis are as follows: Brilliant Green Agar – Isolated Shigella colonies which do not ferment lactose or sucrose and appear red or white in color with no growth to trace growth on the Agar plate will be present. Triple Sugar-Iron Agar – Presence of Shigella will manifest as a red slant with
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Calorimetry and Specific Heat Tessa Williams Chemistry 111 11/13/13 Abstract: In this experiment‚ the specific heat and the density of an unknown metal was determined in order to identify the unknown metal. The average specific heat of the unknown metal was 0.197˚C and was determined using a calorimeter. The density of the unknown metal was 6.57 g/mL and was determined using a cylinder and displacement. Using the specific heat value of the unknown metal and its density‚ it
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