purposely made 1000 times larger then Concentration of CV+. Thus‚ the concentration of OH- changes so little during the reaction that can be assumed to be constant. Materials: IBM Compatible computer‚ serial box interface‚ LoggerPro Software‚ Vernier colorimeter‚ plastic cuvette‚ 250-mL beaker‚ 0.020M NaOH‚ 2 x 10-5 M crystal violet‚ distilled water‚ stirring rod‚ two 10-mL graduated cylinders. Procedure: 1. Obtain and wear safety glasses. 2. Measure out 10 mL of 0.020M NaOH using a 10 mL graduated
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equipment and apparatus used in this experiment: ➢ Colorimeter ➢ Thermometers ➢ Water baths at various temperatures ➢ Stopclock ➢ Test tubes ➢ Small measuring cylinders ➢ Cuvettes ➢ Cork borer ➢ Mounted needles ➢ Beaker about 250 cm³ ➢ Blue Filter The dependent variable was the absorbance. We measured it by using a colorimeter which compares the amount of light getting through a solution
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INTRODUCTION Chemical kinetics‚ which can also be explained as reaction kinetics‚ studies the rates of chemical processes and reactions. A lot of this has to do with the speed at which a chemical reaction occurs for instance the rate at which reactants and products appear and disappear. In this case chemical kinetics goes further in depth to understand what conditions affect the rate of a given reaction. This is important because it allows scientists to determine what type of reaction can occur
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plasma membrane. Overview The beetroot discs will be placed in distilled water at different temperatures. You will compare the intensity of the beetroot pigment that has leaked out from the cells subjectively and possibly objectively using a colorimeter. You can relate the findings to the effect of different temperatures on the permeability of cell membranes in beetroot (as well as in other organisms). Features of experimental design The investigation contains these main features of experimental
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Solution: Beer’s Law Purpose: The purpose of the experiment is to determine the concentration and formula of an unknown cobalt nitrate solution by measuring absorbance. Introduction: A Colorimeter will be used to determine the concentration and formula of an unknown cobalt nitrate solution. The colorimeter sends blue light from the LED light source to pass through the solution and hit a photocell. A solution with a higher concentration will absorb more light and transmits less than s solution
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Investigating Effects of duration of heating on permeability of beetroot cell membrane Introduction: Cell membranes contain many different types of molecules which have different roles in the overall structure of the membrane. Phospholipids form a bilayer‚ which is the basic structure of the membrane. Their non-polar tails form a barrier to most water soluble substances. Membrane proteins serves as channels for transport of metabolites‚ some act as enzymes or carriers‚ while some are receptors
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Aim: To investigate how effect of Detergent Concentration (cont.) has on Membrane permeability of Beetroot cells. Hypothesis: I predict that as detergent concentration increases‚ the solution will become less clear‚ plus mass increases. The increases in mass will indicate that the water potential of the Beetroot cell is lower than that of the surrounding sucrose solution. The Beetroot discs will become flaccid and decrease in mass if the water potential of the surrounding solution is lower than
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The effect of alcohol concentration on the cell membrane Abstract: In this experiment I found out that as the concentration of the alcohol was increased so did the colour intensity of the solution. This is because the higher concentration of ethanol‚ results in more damage done to the cell membrane‚ resulting in leakage of red pigment from the cell. If the membrane is damaged more‚ more red pigment will leak out of the membrane and into the ethanol. Hypothesis: The higher the concentration
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This experiment aims to discover the effect of 6 different treatments on the permeability of the cell membrane. These will be distilled water‚ sucrose‚ sodium hydroxide‚ hydrochloric acid‚ ethanol and boiled water. The treatments include immersion in these solutions‚ and exposure to high temperature. The tissue used is from the taproot of a beetroot‚ and the effect on the membrane’s permeability assessed by the amount of pigment leakage that occurs. Hypothesis ==========
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Investigating the Effect of Temperature on the Permeability of Membranes The permeability of membranes can be altered in several ways. From previous preliminary work I know that reagents‚ for example detergents and alcohol alter the arrangement of the phospholipids in the membrane allowing substances to leak out of the cell. Temperature also affects the membrane. As membranes relay on protein molecules to allow molecules to enter and leave the cell through facilitated diffusion the temperature must
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