Practical 5: determination of activation energy of an enzymed catalyzed reaction Introduction: enzymes are complex chemicals that control reactions in living cells. They are biochemical catalysts‚ speeding up reactions that would occur too slowly to be of any usefulness to an organism. Although in organisms‚ it is not necessary for the reactions to be at maximum rate at all times. Enzymes interact with other molecules to produce a stable system in which the products are made when they are
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Introduction The lung is a spongy organ made up of of irregularly shaped air spaces called Alveoli. The alveoli are lined by a single layer of flat cells and supported by a mesh of fine elastic fibers. The alveoli are surrounded by a rich network of pulmonary capillaries (Couch and Berger‚ 2004). Our lungs are a network of connected tubes that bring oxygen from the air into our blood‚ nourishing the trillions of cells that make up our bodies. The lungs also clean the blood of carbon dioxide waste
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The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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PHOTOSYNTHESIS & CELLUAR RESPIRATION LAB REPORT % Absorption 400 425 450 475 500 525 550 575 600 625 650 675 700 Average 47 44 37 27 14 10 8 9 9 10 15 17 14 Use the graph of Average % Absorption for Grass Pigments to answer the following questions. ANSWER ONLY THE QUESTIONS BELOW. YOU WILL LOSE POINTS IF YOUR ANSWER INCLUDES UNRELATED INFORMATION. 1. What specific range of wavelengths explains why grass is green? The absorption of all wavelengths outside approximately
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Column and Thin Layer Chromatography: The Separation of Spinach Pigment Abstract Spinach extract was separated into fractions containing compounds of similar polarity by column chromatography. Based on solid-liquid phase partitioning‚ this separation technique exploited the different polarity of the compounds in the spinach extract. Three fractions with different colors were obtained. The extract and its fractions were analyzed using thin layer chromatography (TLC). The TLC results showed that
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Biology Coursework ¡V Does The Light Intensity Affect the Rate of Photosynthesis The Investigation In this experiment I will investigate the affect in which the light intensity will have on a plants photosynthesis process. This will be done by measuring the bubbles of oxygen and having a bulb for the light intensity variable. Variables The input variable which will be used in this investigation will be the light intensity (this will be a 100Watt bulb being moved closer and further away from
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Due to the fact that it would be closer to the light source‚ the light would be slightly more intense‚ therefore the rate of photosynthesis would be slightly more advanced‚ causing a lower absorption. I also believed that Sample 1 the sample wrapped in aluminum would have the highest absorbance due to a lack of
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Introduction: Photosynthesis is the process by which green plants use the light energy to convert carbon dioxide and water into simple sugar called glucose‚ which provides a basic energy source for the plant. Photosynthesis occurs in leaves that contain specialized cell structures called chloroplasts. Chloroplasts contain molecules of a green pigment called chlorophyll‚ consisting largely of carbon and hydrogen. It gives green color and absorbs the necessary light for photosynthesis. Chloroplasts are found
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light on the rate of photosynthesis Introduction This experiment was performed to investigate the effects of different wavelengths of light on the rate of photosynthesis. If a multiple colored film petri dishes are place under a 50 watt lamp‚ then the rate of photosynthesis will be greater for those with red‚ blue and clear film‚ than those with green and black film. We believed the petri dishes with the red‚ blue and clear film would produce the best results of photosynthesis as compared to the
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The central message of this advertisement is if you want to be strong like Popeye‚ eat their spinach. I can recall waking up Saturday morning watching the cartoon Popeye as a child. Popeye was always trying to impress Olive Oil. Popeye wasn’t the only one with eyes for Olive Oil‚ his nemesis‚ Pluto also had a very hostile interest towards Olive Oil as well. The cartoon would start out with Popeye planning something nice for Olive Oil‚ the day would be going perfectly. Pluto would notice Olive
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