pH of the alkaline phenol red was 4.0. After adding CO2 by exhaling‚ the color changed from a deep red to a bright red. The pH of the solution also changed; it changed to a 6.0 so it became more neutral by adding CO2 which is more basic then the alkaline phenol red. See Table 2.
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this experiment‚ Egeria densa was observed using a microscope. The task was to observe and identify the different types of cell‚ cytoplasmic streaming‚ and plasmolysis of Egeria densa. First‚ the microscope was examined and investigated to master the use of the equipment. A microscope slide grid which was on the slide glass was required to be seen clearly using 4x‚ 10x and 30x. During the latter part of the experiment‚ the Egeria densa was observed using the microscope to understand the use of microscope
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AP Lab #5 Plant Pigments/Photosynthesis I. Identifying the Effects of Different Variables of Light and Carbon Dioxide on the Rate of Photosynthesis and Observing the Separation of Pigments Through Chromatography II. Introduction Plants have a variety of pigments‚ all of which absorb a different color of light. The three main pigments are chlorophyll a‚ chlorophyll b and carotenoids. Chlorophyll a is the primary plant pigment that absorbs red and blue light‚ which ultimately appears green to the human eye
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The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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Each element emits a unique color light. The purpose of this experiment was to observe these unique colors and calculate their energy. In order to observe this‚ we placed the physical elements in a 24 well plate‚ dipped wet q-tips into the wells to gather the element‚ and put it over the bunsen burner to observe the flame emitted. While observing the different flames‚ we saw that different elements did indeed emit unique flame colors. Colors such as red‚ violet‚ and turquoise were emitted. All of
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Chlorophyll from Fresh Spinach and Investigation of the Photochemistry of Chlorophyll Chlorophyll a Chlorophyll b E5 - Extraction of Chlorophyll from Fresh Spinach E6 - Investigation of the Photochemistry of Chlorophyll The aim of this experiment is to investigate the photochemistry of chlorophyll. This experiment will be performed in two lab periods. In the first lab period you will extract chlorophyll‚ the green pigment in leaves‚ from spinach. In the second lab period you will
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LIGHT IN PHOTOSYNTHESIS Theory: Leaf discs float normally. When the air spaces are infiltrated with the solution the overall density of the leaf disc increases and the disc sinks. The infiltration solution includes a small amount of sodium bicarbonate. The bicarbonate ions serve as the carbon source for photosynthesis. As photosynthesis proceeds oxygen is released into the interior of the leaf which changes the buoyancy – causing the discs to rise. Since cellular respiration is taking place
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Title: Rate of Photosynthesis Introduction: Photosynthesis occurs in plants where the light energy changes to chemical energy. This process occurs in the chloroplast of the plant cell in order to have gas exchange in the organism. Purpose: To measure the effect of light intensity on the rate of photosynthesis in the leaf cells. Hypothesis: The higher the concentration of the sodium bicarbonate solution‚ the faster the leaf of the plant will rise to the surface of the water in the beaker‚ the
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Misael Acosta Photosynthesis Lab Report Ms. Rocha AP Biology 4th Period II. Intro/Background 1. In order for oxygen to be present photosynthesis must occur. Photosynthesis takes in light and CO2‚ and through a series of steps produces oxygen. Photosynthesis can be measured by either the disappearance of substrate or the accumulation of product. Examples include the production of O2 or the consumption of CO2. All the oxygen within the leaf disks will be removed before being placed
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Investigation 5: Photosynthesis Problem: If the leaf disks are treated in a way you know increases the net rate of photosynthesis‚ should they start to float faster or slower? Why? Hypothesis: If the leaf disks were bathed in a red light source‚ then the rate of photosynthesis would increase than leaf disks that are bathed in a regular light source because chlorophyll absorbs red pigment and reflects green pigments. Therefore‚ shining a red light source onto the leaf disks would cause them
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