Paper Chromatography Chromatography comes from the Greek word khromatos (color) and graphein (to write); i.e. paper chromatography is literally color writing on paper. This method tests the purity of compounds and identifies substances. This analytical process‚ despite having been replaced by the success of thin layer chromatography‚ still stands as a valuable teaching tool‚ and is nevertheless very common. This method is very useful because not only is it a relatively quick process‚ but also
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measured in the number of bubbles produced by the plant. Three factors that influence the rate of photosynthesis can be adjusted in the simulator to determine how each of the factors affects the rate of photosynthesis. Virtual Lab PreLab Questions Q: To survive‚ what gas do we need to breathe in? __________________________________ Q: Where is this gas produced? _______________________________ A byproduct of photosynthesis in oxygen gas. Plant use some of the oxygen that they produce‚ but most of
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Paper chromatography Presented by – Miss. Shruti Vilas Kharat. SYBSc / FS13164 History- Paper Chromatography (PC) was first introduced by German scientist Christian Friedrich Schonbein (1865). PC is considered to be the simplest and most widely used of the chromatographic techniques because of its applicability to isolation‚ identification and quantitative determination of organic and inorganic compounds. Definition- Paper chromatography is an analytical method technique for separating and
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Photosynthesis and Cellular Respiration Nina R. McCray May 5‚ 2015 Biology Lab (SCIE207-1502-A-07) LaDonna McCowan-Ferrier Photosynthesis and Cellular Respiration Lab Report Introduction This is an investigation of the effect of the intensity of light (photosynthesis) have on the rate of cellular respiration (measured as the number of oxygen bubbles). We will determine whether or not it is possible to examine the relationship between photosynthesis and cellular respiration under controlled
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Gabriel Alizaidy Liquid Chromatography September 14‚ 2009 Objective: To separate the components of unsweetened‚ grape flavored Kool-Aid. Procedure: Using two syringes‚ inject different concentrations of alcohol to extract red and blue dyes‚ and artificial flavoring. Data: Conclusion: Different dyes are shown when separated by different concentrations of isopropyl alcohol. Discussion of Theory: Chromatography‚ resolution and selectivity played major roles in making the experiment work
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Elodea & Photosynthesis Photosynthesis is the process by which green plants and some other organisms use sunlight to synthesize nutrients from carbon dioxide and water. Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a by-product. Introduction This lab has been created in order to find what extent does distance from a light source (5cm‚ 10cm‚ and 15cm) affect the rate of photosynthesis (measured in bubbles / 3 min) in Elodea water plants. Hypothesis:
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EXPERIMENT 5: CHROMATOGRAPHY Abstract Paper Chromatography uses a chromatogram paper as its stationary phase and the solvent as the mobile phase. Retention factor is the ratio of the distance travelled by the sample to the distance travelled by the solvent. This experiment aims to separate organic compounds‚ to compute Rf values and to identify unknown compounds using Rf values. The ten samples underwent paper chromatography to determine the components of the unknown sample. The mobile phase allowed
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PAPER and COLUMN CHROMATOGRAPHY REPORT 1. 2. The unknown code is an amino acid‚ Glycine. To justify the reason is because the retention factor is the same exact number as the Glycine. The data of the unknown shows everything to be exact with the data of the Glycine 3. The mobile phase is the more polar during the capillary action of the experiment. As soon as the paper touches the mobile phase‚ the solvent rises to the amino acids. This is where you can find polarity of the amino acids.
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Chromatography Abstract Paper chromatography is one of the methods under chromatography‚ it can use in identifying unknown compounds using known compound and it can also use as a separation technique based on the differences in affinities of components of the mixture to a stationary phase and a mobile phase. In the experiment‚ the stationary phase was the filter paper onto which the dye samples were dropped onto while the mobile phase was the solvent mixture containing ethanol and water which
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measured to determine the rate of photosynthesis. This experiment was set up to discover how different wavelengths of light affect the rate of photosynthesis in a spinach‚ DPIP‚ phosphate solution. I proposed that the spinach‚ DPIP‚ phosphate solution under no filter‚ and therefore exposed to all light will have a highest percent orange light transmittance and a highest rate of photosynthesis. The solution under the red filter will have the next highest rate of photosynthesis‚ then the solution under the
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