Photosynthesis is the process in which autotrophs use light energy to convert carbon dioxide and water into glucose. This conversion into chemical energy is associated with the action of the green pigment chlorophyll. Chlorophyll molecules located in the thylakoid membrane of the chloroplasts are arranged into complexes known as photosystems. Each photosystem contains the reaction center complex and is surrounded by light harvesting complexes. The light harvesting complexes are composed of pigment
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Plant Pigment Chromatography VII. Analysis (Questions) 1. What factors are involved in the separation of pigments? Factors that are involved in the separation of pigments can include pigment solubility‚ the attraction between the pigments and paper and the size of each pigment particle. Because of these factors the results were as they were. Beta-carotene traveled the furthest because it forms no hydrogen bonds to the chromatography paper and is slightly soluble in the solvent. Contrastingly
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determining the spectrum of light each absorbs. By using paper chromatography‚ different pigments of spinach leaves can be separated. The knowledge gained in this experiment is relevant to understanding how the process of photosynthesis works. A Real-world application for this includes the harvesting of clean energy sources‚ as scientific advances have led the way to artificial photosynthesis on the path to replace fossil fuels (Nath‚ 237). Photosynthesis converts light received from the sun into chemical
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The purpose of this lab was to understand that because substances have different properties‚ such as mass‚ that can be separated by chromatography. In our experiment‚ we chose 4 different color source to separate in water. We made sure that all the sources were water soluble because only polar substances will dissolve in water. We chose a black wet-erase marker‚ a red marker‚ a green marker‚ and mixed food coloring to test. The chromatography paper was split into 4 sections‚ about 2 cm above the
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Introduction The lung is a spongy organ made up of of irregularly shaped air spaces called Alveoli. The alveoli are lined by a single layer of flat cells and supported by a mesh of fine elastic fibers. The alveoli are surrounded by a rich network of pulmonary capillaries (Couch and Berger‚ 2004). Our lungs are a network of connected tubes that bring oxygen from the air into our blood‚ nourishing the trillions of cells that make up our bodies. The lungs also clean the blood of carbon dioxide waste
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AP Lab #5 Plant Pigments/Photosynthesis I. Identifying the Effects of Different Variables of Light and Carbon Dioxide on the Rate of Photosynthesis and Observing the Separation of Pigments Through Chromatography II. Introduction Plants have a variety of pigments‚ all of which absorb a different color of light. The three main pigments are chlorophyll a‚ chlorophyll b and carotenoids. Chlorophyll a is the primary plant pigment that absorbs red and blue light‚ which ultimately appears green to the human eye
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The results of the food coloring exercise are seen in Figure 1 of the group report. The red food coloring displayed the highest percent transmittance in the long wavelengths closest to red light‚ which was expected. With the same logic‚ it was expected that the blue food coloring would have the highest percent transmittance in
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In this Laboratory experiment‚ my lab partner Alexander and I were able to understand thoroughly the physical and chemical properties of salt (NaCl) and sand (SiO2). Followed by the right procedure we were able to design and test out the components of both NaCl and SiO2 in order to separate the unknown mixture that we were able to find out about. We were also given the task to provide the percent composition of the mixtures. Therefore‚ the separation of components among this experiment allowed us
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Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained‚ with elution 3 containing
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Through this experiment‚ Egeria densa was observed using a microscope. The task was to observe and identify the different types of cell‚ cytoplasmic streaming‚ and plasmolysis of Egeria densa. First‚ the microscope was examined and investigated to master the use of the equipment. A microscope slide grid which was on the slide glass was required to be seen clearly using 4x‚ 10x and 30x. During the latter part of the experiment‚ the Egeria densa was observed using the microscope to understand the
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