BIOLOGY DCN PHOTOSYNTHESIS LAB # 6 Effect of varying coloured filters on the photosynthetic rate of spinach chloroplasts The data below is just representative of what trends and relationships you were supposed to see. Numbers can vary. Absorbance at 620 nm for each treatment DCPIP + chloroplasts t=0 min. 0.93 0.945 0.905 0.915 t=3 min. 0.95 0.731 0.83 0.816 change in A620 -0.02 0.214 0.075 0.099 t=0 min. t=3 min. change in A620 t=0 min. t=3 min. change
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Mobile liquid phase is always passed stationary phase during chromatography. Developed TLC plate is then observed under short wave ultraviolet light‚ where most TLC plates contain fluorescent mixed with silica gel that allow to glow green color. Compounds will absorb the UV light and appear as dark spot against the green color TLC plate. In a column chromatography‚ cotton and sand is placed in purpose of holding silica gel and prevent leakage of adsorbent particles
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The purpose of this lab was to investigate whether veins affect photosynthesis rate. It was predicted that having veins would decrease the rate because chloroplast might be less frequent due to the vascular tissue. For this experiment‚ 4 groups were set up: veins and no veins in CO2 solution‚ and veins and no veins in distilled water‚ the distilled water groups served as controls. These 4 groups were placed under a light bulb in order for photosynthesis to occur. No disks experience movement in the
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Figure 1: Affinity chromatography of fumarase with the Ni2+-NTA-agarose column. Extract (9.9 mL) containing yeast (3.76g) in extraction buffer containing 0.1% Igapel CA-630 and protease inhibitors were pumped through Ni2+-NTA-agarose column. Fractions were collected by 1.5 mL portions by use of wash buffer (20.0 mL)‚ imidazole elution buffer (26.3 mL)‚ and wash buffer (10.0 mL)‚ again. Absorption readings were taken for all fractions with a Cary50 set at 280nm. The fumarase activity was determined
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Chromatography serves mainly as a tool for the examination and separation of mixtures of chemical substances. Chromatography is using a flow of solvent or gas to cause the components of a mixture to migrate differently from a narrow starting point in a specific medium‚ in the case of this experiment‚ filter paper. It is used for the purification and isolation of various substances. There are two phases in chromatography: 1. Stationary Phase – a solid that does not move. In this experiment was the
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Photosynthesis is a crucial energy conversion process that occurs in the chloroplasts of eukaryotic cells. The rate of photosynthetic activity holds importance in environmental‚ horticultural and agricultural situations. (Boardman‚ N.K. 1977). Pigments in the chloroplasts thylakoid membrane absorb the electromagnetic radiation from a light source and release an electron into the electron transport chain. DCPIP’s chemical ability to favourably accept electrons‚ and undergo a colour change from
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Lab Report: Photosynthesis Name: Purpose of this Lab What is the goal of this lab? What question are you trying to answer‚ or what problem are you trying to explain? In this lab I will perform experiments to explain photosynthesis. I will answer questions like what does photosynthesis do and how does photosynthesis work? Hypothesis After reading the lab instructions (but before starting the lab) record your best educated guess about what will happen in the experiment. Explain
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strong‚ a foundation known as photosynthesis. When photosynthesis is functioning as it should‚ in its fullest capacity‚ all organisms benefit from it in some way. Furthermore‚ scientists aim to better understand the photosynthetic process to help enhance the efficiency and quality of life on Earth. By fully understanding the function of photosynthesis‚ some environmental adjustments can then be made to refine the photosynthetic process. In this lab specifically (Lab No. 7)‚ we isolated specific color
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An Experiment to Test the Effect of Temperature on the Rate of Photosynthesis in Green Algae Background Photosynthesis is an amazing process where plants are able to create their own food as well as oxygen using sunlight‚ carbon dioxide (co2)‚ and water. The part of the plant responsible for photosynthesis is the enzymes in the chlorophyll (structures that carry out photosynthesis located in leaves). Photosynthesis is the process of creating glucose. Water + Carbon Dioxide + Sunlight = Glucose
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Name: (e.g.‚ BioLab 3.4_A_Jones.doc) MDean Date: Graded Assignment Lab Report Answer the questions. When you are finished‚ submit this assignment to your teacher by the due date for full credit. (5 points) 1. Describe generally what happened to each spot of each type of ink. Which had the most pigments? Answer: The black ink went from a light blue to dark blue then to red. The red ink went from red to pink. It went the furthest out of the inks. The green ink went from dark blue to green and then
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