Chromatography of Photosynthetic Pigments Abstract In this experiment a process of chromatography was used to separate chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and beta carotene. When these pigments were obtained they were used to measure the wavelengths by way of spectrophotometer of each and the total of all the pigments wavelength. Introduction Photosynthesis is a process by which plants use the sunlight to convert it from light energy into chemical energy. This equation shows us how
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Course & Year: BS Biotech-2 Lab Schedule: W (1-4) Group 5 Date Performed: Dec. 12‚ 2012 Date Submitted: Jan. 9‚ 2013 Separation of Photosynthetic Pigment by Paper Chromatography I. INTRODUCTION There are different types of components in plant pigments. The most important and abundant chemical pigment found in plants is chlorophyll. This pigment exists in two forms; chlorophyll a and chlorophyll b. Chlorophyll a‚ being the main photosynthetic pigment‚ has a primary purpose to convert
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BIOLOGY LAB REPORT NAME :princess cucumber TOPIC : 3.8 & 8.2 : PHOTOSYNTHETIC PIGMENTS RESEARCH QUESTION INTRODUCTION The photosynthetic pigments are responsible for absorbing and trapping light energy the major pigments of photosynthesis are the chlorophylls. The two chlorophylls found in green plants are chlorophyll a and chlorophyll b. other pigments include carotenoids and phycobilins‚ sometimes referred to as accessory pigments. Carotenoids occur in all photosynthetic organisms‚ while
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NOTES AND COMMENT 343 DETERMINATION OF CHLOROPHYLL AND PHEO-PIGMENTS : SPECTROPHOTOMETRIC EQUATIONS~ It has been shown that chlorophyll degradation products may at times constitute a significant fraction of the total green pigments present in seawater (Yentsch and Menzel 1963; Lorenzen 1965; Yentsch 1965). These degraded forms‚ or inactive chlorophyll‚ absorb light in the red part of the spectrum; if they are present in concentrations significant relative to chlorophyll a‚ a serious error
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intensity on the Ceratophyllum ‘s photosynthetic rate Name:negar mir sharifi Student number:212656302 Lab section:D4
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Lab 11- Plant pigment and Photosynthesis Zelda Salfati December 20th‚ 2012 BIOL 1012Y Partners Lab: Nicole Mintz‚ Bayla Kronman
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purpose of this lab was to separate plant pigments using chromatography‚ calculate Rf values using the collected data‚ and study photosynthesis with isolated chloroplasts. Light energy Light energy Background Information (Activity A): In photosynthesis‚ plant cells convert light energy into chemical energy that is stored in sugars and other organic compounds. It is an endergonic and anaerobic reaction. Critical to the process is chlorophyll‚ the primary photosynthetic pigment in chloroplasts.
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The purpose of this paper is to help analyze or compare pigments from two organisms. Chromatogram is the pattern that the chromatography paper has created from its substances. A retention factor‚ also abbreviated as an Rf value‚ is the measurement of the distance that the sample had traveled. The formula for an Rf value is migration distance of solvent front divided by migration distance of substance. An Rf value is the distance the pigment travels from the original spot‚ which is the dot‚ of solvent
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BEETROOT PIGMENTS - and membranes – These pigments are betalain pigments (not‚ as often thought‚ anthocyanins)‚ which they replace in some organisms. They are named after the Beet family of plants (Beta) but are also found in fungi (Fly Agaric - the red‚ spotted one!). In petals they presumably attract pollinating insects and may be present in seeds/fruits to encourage birds to eat them and so disperse the seeds. Man has selected for colour in beetroot‚ both because
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The History of Paint Pigments Chem 111-404 April 24‚ 2013 Introduction: This report expresses five different pigments that were formed during chemical reactions. Three out of the total five reactions went through a metathesis‚ or double-replacment reaction. A metathesis reaction can be defined as “a chemical reaction in which an element or radical in one compound exchanges places with another element or radical in another compound.” (Webster). The other two pigments underwent a neutralization
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