Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
Premium Chemistry Concentration Solution
form the structures of cells but they act as enzymes in reactions of the body1. Enzymes are biological catalysts that lower the amount of activation energy needed in carrying out biochemical reactions1. Enzymes are responsible for almost every reaction that occurs in a cell and is named according to the substrate they specifically affect. An enzyme works best under optimal conditions pertaining to temperature‚ pH level and salt concentration1. In unfavorable conditions enzymes will become denatured
Premium Protein Metabolism DNA
Ph Lab Report Bryon Kim 123013 B(2) Biology Background information/Research PH paper (litmus paper) determines how acidic or how basic a substance is. The paper changes color accordingly to color code on the pH scale. The pH scale starts from 0 to 14. The lower the number the more acidic it is. Zero is the most acidic‚ and 14 is the most basic while 7 is the neutral number for example water. Examples of an acid is lemon juice or multi purpose cleaner. Examples of a basic substance is shampoo or liquid soap
Premium Green Purple Blue
EXPERIMENT Materials 200 Toothpicks Timer Tape Controls 50 toothpicks per trial. 120 seconds per trial. The same brand of toothpicks. One toothpick broken at a time (except for Mutation Trial 2). One toothpick broken into two pieces equals one reaction. Broken toothpicks cannot react again. (Toothpicks can only be broken once) The toothpicks are broken between the thumb‚ index‚ and middle finger (toothpickase). Break two toothpicks at a time (Trail 3). Tape the index finger and thumb. (Trail
Premium Enzyme Chemical reaction Finger
A company has specified that the acceptable size of a knob that is to be produced is (0.140 ± 0.003) inches. In this lab‚ the dimensions of the knobs produced in one day are tested for discrepancy in order to pass control testing. This lab also reports the probability that knobs with the acceptable dimension will be produced. MATERIALS AND METHODS Using Microsoft Excel‚ a histogram was created displaying the three documented dimensions of 15 batches of knobs. This histogram was obtained in order
Premium Measurement Test method Control theory
ISSUES The author is currently attached to the x’s U.S. Funds’ Compliance constituting seven members that report to two directors and overseen by a Global Compliance Director. The organization structure of x is as follows: With the current on-goings in x‚ survey among employees’ shows that the management issues that is faced by the company are absence in leadership‚ lack of management skills‚ unsuitable organization structure‚ and communication breakdown amongst team members. Lack of Leadership
Premium Management
Based on the prompt above‚ “You are going to test your body’s ability to maintain homeostasis. You will test this by monitoring a person in your group’s breathing rate‚ heart rate‚ and qualitative observations” we devised a hypothesis which states that if a person exercises‚ his/her heart rate‚ breathing rate and sweat (qualitative observation) will increase in order for our bodies to maintain homeostasis and control our bodily functions. Based on our experiment we found that in Trial 1 the heart
Premium Heart Oxygen Physical exercise
University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
Premium Protein Chromatography Analytical chemistry
Unknown Lab Report #1 Unknown #1 April 25‚ 2012 Microbiology Spring 2012 MCB2010C Unknown #1 Introduction Identity of a microorganism has proven to be very significant. Doing so can help identify diseases and created treatment and cures for such diseases. As a result‚ various laboratory tests were performed to an unknown microbe (Unknown #1) found in the water of a nearby pond. By identify the microbe‚ the safety of the water will be known to those around it. Materials and Methods
Premium
Engine Lab Report Diesel Engine Load/N |Fuel Time/s |dH/mmH2O |Speed/r.p.m |Temp/℃ |Air consumption/kg/H |Fuel consumption/kg/H |Air-fuel ratio |Power/kw |Efficiency/ % | |40 |121.6 |17.5 |3018 |26.6 |130.16 |2.47 |52.7 |4.5 |0.019 | |80 |94.72 |17.5 |3009 |26.7 |130.14 |3.17 |41.05 |8.97 |0.059 | |125 |72.76 |17 |3009 |26.8 |128.25 |4.12 |31.13 |14.02 |0.111 | |171 |56.95 |17 |3000 |26.9 |128.23 |5.72 |24.33 |19.12 |0.161 | |212 |46.06 |16.5 |3006 |27.1 |126.28 |6.51 |19.40 |23.76 |0.202 | |232
Premium Internal combustion engine Fuel injection Diesel engine